Mycobacterium phage Terror
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D8EVZ3|A0A1D8EVZ3_9CAUD Uncharacterized protein OS=Mycobacterium phage Terror OX=1897550 GN=37 PE=4 SV=1
MM1 pKa = 7.85 AEE3 pKa = 4.44 DD4 pKa = 4.05 RR5 pKa = 11.84 DD6 pKa = 4.42 GPMSTIDD13 pKa = 3.53 AEE15 pKa = 4.44 NPRR18 pKa = 11.84 SGWSAEE24 pKa = 4.16 CHH26 pKa = 5.86 DD27 pKa = 4.05 CPYY30 pKa = 10.64 RR31 pKa = 11.84 QLEE34 pKa = 4.29 GVDD37 pKa = 3.33 AA38 pKa = 5.14
Molecular weight: 4.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.16
IPC2_protein 4.177
IPC_protein 4.012
Toseland 3.834
ProMoST 4.177
Dawson 3.999
Bjellqvist 4.164
Wikipedia 3.948
Rodwell 3.859
Grimsley 3.757
Solomon 3.973
Lehninger 3.935
Nozaki 4.139
DTASelect 4.317
Thurlkill 3.897
EMBOSS 3.948
Sillero 4.139
Patrickios 3.249
IPC_peptide 3.973
IPC2_peptide 4.113
IPC2.peptide.svr19 4.042
Protein with the highest isoelectric point:
>tr|A0A1D8EVZ0|A0A1D8EVZ0_9CAUD Tail assembly chaperone protein OS=Mycobacterium phage Terror OX=1897550 GN=15 PE=4 SV=1
MM1 pKa = 7.29 IWEE4 pKa = 5.41 PILAALGGYY13 pKa = 10.12 LFFAGRR19 pKa = 11.84 DD20 pKa = 3.51 VFRR23 pKa = 11.84 SVMQSEE29 pKa = 4.4 WMFGRR34 pKa = 11.84 ILAADD39 pKa = 4.21 LRR41 pKa = 11.84 AIDD44 pKa = 3.29 RR45 pKa = 11.84 AQRR48 pKa = 11.84 RR49 pKa = 11.84 KK50 pKa = 10.45 AKK52 pKa = 10.39
Molecular weight: 6.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.677
IPC_protein 10.804
Toseland 10.935
ProMoST 11.052
Dawson 10.979
Bjellqvist 10.804
Wikipedia 11.301
Rodwell 10.979
Grimsley 11.023
Solomon 11.257
Lehninger 11.199
Nozaki 10.906
DTASelect 10.804
Thurlkill 10.921
EMBOSS 11.359
Sillero 10.935
Patrickios 10.833
IPC_peptide 11.272
IPC2_peptide 9.926
IPC2.peptide.svr19 8.854
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
60
0
60
13358
38
1341
222.6
23.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.258 ± 0.601
0.853 ± 0.131
6.932 ± 0.302
5.652 ± 0.433
2.987 ± 0.193
9.215 ± 0.492
2.126 ± 0.238
4.447 ± 0.203
3.698 ± 0.271
7.546 ± 0.262
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.163 ± 0.117
3.099 ± 0.2
6.251 ± 0.405
3.354 ± 0.142
6.738 ± 0.482
5.128 ± 0.353
5.981 ± 0.241
6.7 ± 0.236
1.961 ± 0.162
1.909 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here