Zostera marina amalgavirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Amalgaviridae; unclassified Amalgaviridae

Average proteome isoelectric point is 8.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1W6R5F0|A0A1W6R5F0_9VIRU Putative coat protein OS=Zostera marina amalgavirus 1 OX=1985202 PE=4 SV=1
MM1 pKa = 7.86AEE3 pKa = 4.71DD4 pKa = 4.62LRR6 pKa = 11.84SQQTLDD12 pKa = 4.41DD13 pKa = 4.27RR14 pKa = 11.84DD15 pKa = 3.9NLKK18 pKa = 10.9LLTDD22 pKa = 4.08AFKK25 pKa = 10.6AYY27 pKa = 9.65PEE29 pKa = 4.95GGLSVVDD36 pKa = 4.12VSLEE40 pKa = 4.15GIAACNYY47 pKa = 6.33TVSRR51 pKa = 11.84AVKK54 pKa = 9.85VMKK57 pKa = 10.04ILKK60 pKa = 9.25PLTKK64 pKa = 10.03NEE66 pKa = 3.86HH67 pKa = 6.22LIKK70 pKa = 10.47LFHH73 pKa = 6.38YY74 pKa = 10.79ANDD77 pKa = 3.25VSNISSILPMTLEE90 pKa = 4.49TNFKK94 pKa = 8.5FCKK97 pKa = 9.62WLTSPVAKK105 pKa = 10.29KK106 pKa = 10.45KK107 pKa = 10.21IAALQNADD115 pKa = 3.56RR116 pKa = 11.84LRR118 pKa = 11.84RR119 pKa = 11.84RR120 pKa = 11.84GSDD123 pKa = 3.08VVTPEE128 pKa = 3.75EE129 pKa = 3.76AAMIALLEE137 pKa = 4.21SAQADD142 pKa = 3.97RR143 pKa = 11.84TTEE146 pKa = 3.86YY147 pKa = 10.44SKK149 pKa = 11.34ARR151 pKa = 11.84ARR153 pKa = 11.84YY154 pKa = 9.35DD155 pKa = 3.14IDD157 pKa = 3.37VAKK160 pKa = 10.4YY161 pKa = 9.17KK162 pKa = 10.79KK163 pKa = 10.3KK164 pKa = 8.77IAKK167 pKa = 7.74RR168 pKa = 11.84TRR170 pKa = 11.84QLEE173 pKa = 3.88EE174 pKa = 5.19DD175 pKa = 3.74LDD177 pKa = 4.27KK178 pKa = 11.69VQASYY183 pKa = 10.83PGLQLIEE190 pKa = 4.79RR191 pKa = 11.84PDD193 pKa = 3.62EE194 pKa = 4.36HH195 pKa = 9.05SVMASAWHH203 pKa = 6.72RR204 pKa = 11.84YY205 pKa = 7.58VDD207 pKa = 4.67FCTSNNFEE215 pKa = 4.29VPQKK219 pKa = 11.16NDD221 pKa = 2.85GNLARR226 pKa = 11.84AYY228 pKa = 9.69KK229 pKa = 9.92QFEE232 pKa = 4.26RR233 pKa = 11.84EE234 pKa = 3.8LTLEE238 pKa = 4.29IKK240 pKa = 9.21NTACQKK246 pKa = 10.61PEE248 pKa = 3.65VRR250 pKa = 11.84DD251 pKa = 3.66YY252 pKa = 11.57LLQYY256 pKa = 10.49CKK258 pKa = 10.72EE259 pKa = 3.98KK260 pKa = 11.17VKK262 pKa = 10.62GFRR265 pKa = 11.84AQFEE269 pKa = 4.55DD270 pKa = 4.96KK271 pKa = 10.56RR272 pKa = 11.84AQTYY276 pKa = 9.35RR277 pKa = 11.84GYY279 pKa = 9.81YY280 pKa = 9.83ACATGDD286 pKa = 3.98ILPEE290 pKa = 3.73DD291 pKa = 4.05TPRR294 pKa = 11.84KK295 pKa = 8.88KK296 pKa = 10.46RR297 pKa = 11.84KK298 pKa = 9.53ASRR301 pKa = 11.84PDD303 pKa = 3.05TGGEE307 pKa = 3.91ANQEE311 pKa = 4.06GEE313 pKa = 4.52DD314 pKa = 3.95DD315 pKa = 4.39SEE317 pKa = 4.94HH318 pKa = 7.56SVIPNLLDD326 pKa = 3.24TCVGDD331 pKa = 6.28DD332 pKa = 3.34RR333 pKa = 11.84RR334 pKa = 11.84AWDD337 pKa = 3.63KK338 pKa = 8.73THH340 pKa = 6.67PTNPEE345 pKa = 3.58KK346 pKa = 10.61PGNDD350 pKa = 3.23SSSDD354 pKa = 3.26EE355 pKa = 4.24SAEE358 pKa = 3.98NQPASTVADD367 pKa = 3.8RR368 pKa = 11.84TRR370 pKa = 11.84TRR372 pKa = 11.84RR373 pKa = 11.84RR374 pKa = 11.84KK375 pKa = 9.68ADD377 pKa = 3.23KK378 pKa = 10.29HH379 pKa = 5.83SKK381 pKa = 9.49KK382 pKa = 10.73

Molecular weight:
43.43 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1W6R5F0|A0A1W6R5F0_9VIRU Putative coat protein OS=Zostera marina amalgavirus 1 OX=1985202 PE=4 SV=1
MM1 pKa = 7.86AEE3 pKa = 4.71DD4 pKa = 4.62LRR6 pKa = 11.84SQQTLDD12 pKa = 4.41DD13 pKa = 4.27RR14 pKa = 11.84DD15 pKa = 3.9NLKK18 pKa = 10.9LLTDD22 pKa = 4.08AFKK25 pKa = 10.6AYY27 pKa = 9.65PEE29 pKa = 4.95GGLSVVDD36 pKa = 4.12VSLEE40 pKa = 4.15GIAACNYY47 pKa = 6.33TVSRR51 pKa = 11.84AVKK54 pKa = 9.85VMKK57 pKa = 10.04ILKK60 pKa = 9.25PLTKK64 pKa = 10.03NEE66 pKa = 3.86HH67 pKa = 6.22LIKK70 pKa = 10.47LFHH73 pKa = 6.38YY74 pKa = 10.79ANDD77 pKa = 3.25VSNISSILPMTLEE90 pKa = 4.49TNFKK94 pKa = 8.5FCKK97 pKa = 9.62WLTSPVAKK105 pKa = 10.29KK106 pKa = 10.45KK107 pKa = 10.21IAALQNADD115 pKa = 3.56RR116 pKa = 11.84LRR118 pKa = 11.84RR119 pKa = 11.84RR120 pKa = 11.84GSDD123 pKa = 3.08VVTPEE128 pKa = 3.75EE129 pKa = 3.76AAMIALLEE137 pKa = 4.21SAQADD142 pKa = 3.97RR143 pKa = 11.84TTEE146 pKa = 3.86YY147 pKa = 10.44SKK149 pKa = 11.34ARR151 pKa = 11.84ARR153 pKa = 11.84YY154 pKa = 9.35DD155 pKa = 3.14IDD157 pKa = 3.37VAKK160 pKa = 10.4YY161 pKa = 9.17KK162 pKa = 10.79KK163 pKa = 10.3KK164 pKa = 8.77IAKK167 pKa = 7.74RR168 pKa = 11.84TRR170 pKa = 11.84QLEE173 pKa = 3.88EE174 pKa = 5.19DD175 pKa = 3.74LDD177 pKa = 4.27KK178 pKa = 11.69VQASYY183 pKa = 10.83PGLQLIEE190 pKa = 4.79RR191 pKa = 11.84PDD193 pKa = 3.62EE194 pKa = 4.36HH195 pKa = 9.05SVMASAWHH203 pKa = 6.72RR204 pKa = 11.84YY205 pKa = 7.58VDD207 pKa = 4.67FCTSNNFEE215 pKa = 4.29VPQKK219 pKa = 11.16NDD221 pKa = 2.85GNLARR226 pKa = 11.84AYY228 pKa = 9.69KK229 pKa = 9.92QFEE232 pKa = 4.26RR233 pKa = 11.84EE234 pKa = 3.8LTLEE238 pKa = 4.29IKK240 pKa = 9.21NTACQKK246 pKa = 10.61PEE248 pKa = 3.65VRR250 pKa = 11.84DD251 pKa = 3.66YY252 pKa = 11.57LLQYY256 pKa = 10.49CKK258 pKa = 10.6EE259 pKa = 4.05KK260 pKa = 11.29VKK262 pKa = 10.93GFVPNSKK269 pKa = 8.94TKK271 pKa = 8.86EE272 pKa = 3.87LKK274 pKa = 10.89LIGDD278 pKa = 3.96TMHH281 pKa = 7.02ARR283 pKa = 11.84LEE285 pKa = 4.54TYY287 pKa = 10.11FLRR290 pKa = 11.84IPLEE294 pKa = 3.79RR295 pKa = 11.84RR296 pKa = 11.84GRR298 pKa = 11.84LLDD301 pKa = 3.73QIPVGRR307 pKa = 11.84LTRR310 pKa = 11.84KK311 pKa = 10.23GKK313 pKa = 6.49MTPNIPLSQIFSTPALGMTAVHH335 pKa = 6.04GTKK338 pKa = 8.39PTPPIQRR345 pKa = 11.84NLEE348 pKa = 4.04MTLLPMNLLKK358 pKa = 10.37TNLRR362 pKa = 11.84VQLLTEE368 pKa = 4.2HH369 pKa = 5.92VRR371 pKa = 11.84GGVRR375 pKa = 11.84QISIARR381 pKa = 11.84SKK383 pKa = 10.1WEE385 pKa = 3.86AGVRR389 pKa = 11.84RR390 pKa = 11.84IIGGGEE396 pKa = 3.5MRR398 pKa = 11.84GWEE401 pKa = 4.42KK402 pKa = 11.24DD403 pKa = 3.2SALYY407 pKa = 10.43RR408 pKa = 11.84GGGNLHH414 pKa = 6.71DD415 pKa = 6.08AIRR418 pKa = 11.84LLATGRR424 pKa = 11.84QDD426 pKa = 3.31PPGSFLFEE434 pKa = 4.18HH435 pKa = 6.93FSLEE439 pKa = 4.04TARR442 pKa = 11.84EE443 pKa = 3.86ILLLPCNLEE452 pKa = 4.14VPDD455 pKa = 4.27GPKK458 pKa = 10.07AVRR461 pKa = 11.84MKK463 pKa = 10.91NFNEE467 pKa = 4.48DD468 pKa = 2.94ATAGPVLRR476 pKa = 11.84ALRR479 pKa = 11.84CKK481 pKa = 10.31SKK483 pKa = 11.09YY484 pKa = 9.35GLKK487 pKa = 10.48EE488 pKa = 3.64GLEE491 pKa = 4.23RR492 pKa = 11.84IAWDD496 pKa = 3.67LYY498 pKa = 11.48DD499 pKa = 3.92RR500 pKa = 11.84VGDD503 pKa = 3.65GKK505 pKa = 10.63LRR507 pKa = 11.84RR508 pKa = 11.84WQLPPLLARR517 pKa = 11.84IGFRR521 pKa = 11.84SKK523 pKa = 10.74LVEE526 pKa = 3.83QEE528 pKa = 4.1SAIKK532 pKa = 10.25KK533 pKa = 9.45IFSGQPIGRR542 pKa = 11.84AVMMLDD548 pKa = 4.01AMEE551 pKa = 4.57QPFSSPLYY559 pKa = 10.05NALCDD564 pKa = 3.81VVSKK568 pKa = 10.59LNRR571 pKa = 11.84VPEE574 pKa = 4.38SGWRR578 pKa = 11.84NMVVRR583 pKa = 11.84ASSDD587 pKa = 2.51WSRR590 pKa = 11.84FWGDD594 pKa = 2.93IRR596 pKa = 11.84NSCAIVEE603 pKa = 4.32LDD605 pKa = 2.84WSKK608 pKa = 11.02FDD610 pKa = 4.12RR611 pKa = 11.84EE612 pKa = 4.14RR613 pKa = 11.84PKK615 pKa = 11.03EE616 pKa = 4.32DD617 pKa = 2.98IEE619 pKa = 4.16FVINIFVSCFLPKK632 pKa = 10.04NRR634 pKa = 11.84RR635 pKa = 11.84EE636 pKa = 3.88RR637 pKa = 11.84RR638 pKa = 11.84LLSAYY643 pKa = 9.69KK644 pKa = 10.68YY645 pKa = 9.71MMRR648 pKa = 11.84KK649 pKa = 9.51ALIDD653 pKa = 3.74KK654 pKa = 10.73VIMLDD659 pKa = 3.53DD660 pKa = 4.38GCAFTYY666 pKa = 10.8DD667 pKa = 3.34GMIPSGSLWTGLLGTALNILYY688 pKa = 10.19INAAVRR694 pKa = 11.84RR695 pKa = 11.84LGIPSDD701 pKa = 3.7EE702 pKa = 4.15FVPYY706 pKa = 10.42CAGDD710 pKa = 3.79DD711 pKa = 3.57NLTVFKK717 pKa = 10.63YY718 pKa = 9.06PQRR721 pKa = 11.84ATRR724 pKa = 11.84LGKK727 pKa = 9.78IRR729 pKa = 11.84GMLNEE734 pKa = 4.34MFRR737 pKa = 11.84AGIDD741 pKa = 3.39PEE743 pKa = 4.75DD744 pKa = 4.96FIIHH748 pKa = 5.99YY749 pKa = 9.89PPFHH753 pKa = 5.4VTKK756 pKa = 10.5VQAKK760 pKa = 8.74FPAGFDD766 pKa = 3.73FTHH769 pKa = 6.37GTSKK773 pKa = 10.72FLDD776 pKa = 3.37QCQWIPLKK784 pKa = 11.25GNIHH788 pKa = 5.99VDD790 pKa = 3.03HH791 pKa = 6.99SEE793 pKa = 4.12GLSHH797 pKa = 7.11RR798 pKa = 11.84WNYY801 pKa = 8.62VFKK804 pKa = 10.94GKK806 pKa = 9.64PKK808 pKa = 10.08FLANYY813 pKa = 9.2FMEE816 pKa = 5.62DD817 pKa = 3.09GRR819 pKa = 11.84SIRR822 pKa = 11.84PAHH825 pKa = 6.99DD826 pKa = 3.57NLEE829 pKa = 4.12KK830 pKa = 10.79LLYY833 pKa = 9.45PEE835 pKa = 5.23NVQQKK840 pKa = 9.77IEE842 pKa = 4.26DD843 pKa = 3.69YY844 pKa = 9.31EE845 pKa = 4.23AAVLSMAVDD854 pKa = 4.03NPWNSHH860 pKa = 5.81NINHH864 pKa = 6.62LMQRR868 pKa = 11.84FCIIQQIKK876 pKa = 8.86RR877 pKa = 11.84QAVHH881 pKa = 7.18PLTAGDD887 pKa = 4.06VLFCAKK893 pKa = 9.85FQSISEE899 pKa = 4.23NMWMFPTVGAWRR911 pKa = 11.84RR912 pKa = 11.84QLLNVRR918 pKa = 11.84MEE920 pKa = 5.35DD921 pKa = 3.49IEE923 pKa = 4.21EE924 pKa = 4.22LKK926 pKa = 9.61PTVEE930 pKa = 4.11AFSEE934 pKa = 4.7FVRR937 pKa = 11.84GVTSLYY943 pKa = 10.86SRR945 pKa = 11.84ASTGGLDD952 pKa = 2.73SWQFMEE958 pKa = 4.18ILRR961 pKa = 11.84GEE963 pKa = 4.07RR964 pKa = 11.84DD965 pKa = 3.48AGSGQYY971 pKa = 10.92GSDD974 pKa = 3.09IEE976 pKa = 4.27AWCAFLNRR984 pKa = 11.84NGLTRR989 pKa = 11.84SLRR992 pKa = 11.84PAKK995 pKa = 10.15RR996 pKa = 11.84YY997 pKa = 9.64RR998 pKa = 11.84KK999 pKa = 9.29EE1000 pKa = 3.62KK1001 pKa = 9.1QTYY1004 pKa = 8.42QSAEE1008 pKa = 3.99AGDD1011 pKa = 4.46KK1012 pKa = 10.82ILVSLAAIYY1021 pKa = 7.63ATSDD1025 pKa = 2.92YY1026 pKa = 11.23DD1027 pKa = 3.96LRR1029 pKa = 11.84EE1030 pKa = 4.24DD1031 pKa = 4.02RR1032 pKa = 11.84DD1033 pKa = 3.54LGFIIRR1039 pKa = 11.84ISNEE1043 pKa = 3.24LRR1045 pKa = 11.84KK1046 pKa = 9.61QLLL1049 pKa = 3.44

Molecular weight:
119.87 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1431

382

1049

715.5

81.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.036 ± 0.904

1.607 ± 0.123

6.499 ± 1.03

6.499 ± 0.456

3.704 ± 0.739

5.451 ± 0.979

1.817 ± 0.009

5.101 ± 0.931

7.407 ± 1.106

9.853 ± 1.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.376 ± 0.585

4.333 ± 0.064

4.682 ± 0.127

3.704 ± 0.122

8.036 ± 0.1

5.94 ± 0.188

4.822 ± 0.801

5.381 ± 0.08

1.468 ± 0.374

3.284 ± 0.209

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski