Zostera marina amalgavirus 1
Average proteome isoelectric point is 8.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6R5F0|A0A1W6R5F0_9VIRU Putative coat protein OS=Zostera marina amalgavirus 1 OX=1985202 PE=4 SV=1
MM1 pKa = 7.86 AEE3 pKa = 4.71 DD4 pKa = 4.62 LRR6 pKa = 11.84 SQQTLDD12 pKa = 4.41 DD13 pKa = 4.27 RR14 pKa = 11.84 DD15 pKa = 3.9 NLKK18 pKa = 10.9 LLTDD22 pKa = 4.08 AFKK25 pKa = 10.6 AYY27 pKa = 9.65 PEE29 pKa = 4.95 GGLSVVDD36 pKa = 4.12 VSLEE40 pKa = 4.15 GIAACNYY47 pKa = 6.33 TVSRR51 pKa = 11.84 AVKK54 pKa = 9.85 VMKK57 pKa = 10.04 ILKK60 pKa = 9.25 PLTKK64 pKa = 10.03 NEE66 pKa = 3.86 HH67 pKa = 6.22 LIKK70 pKa = 10.47 LFHH73 pKa = 6.38 YY74 pKa = 10.79 ANDD77 pKa = 3.25 VSNISSILPMTLEE90 pKa = 4.49 TNFKK94 pKa = 8.5 FCKK97 pKa = 9.62 WLTSPVAKK105 pKa = 10.29 KK106 pKa = 10.45 KK107 pKa = 10.21 IAALQNADD115 pKa = 3.56 RR116 pKa = 11.84 LRR118 pKa = 11.84 RR119 pKa = 11.84 RR120 pKa = 11.84 GSDD123 pKa = 3.08 VVTPEE128 pKa = 3.75 EE129 pKa = 3.76 AAMIALLEE137 pKa = 4.21 SAQADD142 pKa = 3.97 RR143 pKa = 11.84 TTEE146 pKa = 3.86 YY147 pKa = 10.44 SKK149 pKa = 11.34 ARR151 pKa = 11.84 ARR153 pKa = 11.84 YY154 pKa = 9.35 DD155 pKa = 3.14 IDD157 pKa = 3.37 VAKK160 pKa = 10.4 YY161 pKa = 9.17 KK162 pKa = 10.79 KK163 pKa = 10.3 KK164 pKa = 8.77 IAKK167 pKa = 7.74 RR168 pKa = 11.84 TRR170 pKa = 11.84 QLEE173 pKa = 3.88 EE174 pKa = 5.19 DD175 pKa = 3.74 LDD177 pKa = 4.27 KK178 pKa = 11.69 VQASYY183 pKa = 10.83 PGLQLIEE190 pKa = 4.79 RR191 pKa = 11.84 PDD193 pKa = 3.62 EE194 pKa = 4.36 HH195 pKa = 9.05 SVMASAWHH203 pKa = 6.72 RR204 pKa = 11.84 YY205 pKa = 7.58 VDD207 pKa = 4.67 FCTSNNFEE215 pKa = 4.29 VPQKK219 pKa = 11.16 NDD221 pKa = 2.85 GNLARR226 pKa = 11.84 AYY228 pKa = 9.69 KK229 pKa = 9.92 QFEE232 pKa = 4.26 RR233 pKa = 11.84 EE234 pKa = 3.8 LTLEE238 pKa = 4.29 IKK240 pKa = 9.21 NTACQKK246 pKa = 10.61 PEE248 pKa = 3.65 VRR250 pKa = 11.84 DD251 pKa = 3.66 YY252 pKa = 11.57 LLQYY256 pKa = 10.49 CKK258 pKa = 10.72 EE259 pKa = 3.98 KK260 pKa = 11.17 VKK262 pKa = 10.62 GFRR265 pKa = 11.84 AQFEE269 pKa = 4.55 DD270 pKa = 4.96 KK271 pKa = 10.56 RR272 pKa = 11.84 AQTYY276 pKa = 9.35 RR277 pKa = 11.84 GYY279 pKa = 9.81 YY280 pKa = 9.83 ACATGDD286 pKa = 3.98 ILPEE290 pKa = 3.73 DD291 pKa = 4.05 TPRR294 pKa = 11.84 KK295 pKa = 8.88 KK296 pKa = 10.46 RR297 pKa = 11.84 KK298 pKa = 9.53 ASRR301 pKa = 11.84 PDD303 pKa = 3.05 TGGEE307 pKa = 3.91 ANQEE311 pKa = 4.06 GEE313 pKa = 4.52 DD314 pKa = 3.95 DD315 pKa = 4.39 SEE317 pKa = 4.94 HH318 pKa = 7.56 SVIPNLLDD326 pKa = 3.24 TCVGDD331 pKa = 6.28 DD332 pKa = 3.34 RR333 pKa = 11.84 RR334 pKa = 11.84 AWDD337 pKa = 3.63 KK338 pKa = 8.73 THH340 pKa = 6.67 PTNPEE345 pKa = 3.58 KK346 pKa = 10.61 PGNDD350 pKa = 3.23 SSSDD354 pKa = 3.26 EE355 pKa = 4.24 SAEE358 pKa = 3.98 NQPASTVADD367 pKa = 3.8 RR368 pKa = 11.84 TRR370 pKa = 11.84 TRR372 pKa = 11.84 RR373 pKa = 11.84 RR374 pKa = 11.84 KK375 pKa = 9.68 ADD377 pKa = 3.23 KK378 pKa = 10.29 HH379 pKa = 5.83 SKK381 pKa = 9.49 KK382 pKa = 10.73
Molecular weight: 43.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.532
IPC2_protein 7.878
IPC_protein 7.761
Toseland 7.658
ProMoST 8.419
Dawson 8.565
Bjellqvist 8.726
Wikipedia 8.521
Rodwell 8.624
Grimsley 7.629
Solomon 8.668
Lehninger 8.668
Nozaki 8.916
DTASelect 8.536
Thurlkill 8.624
EMBOSS 8.756
Sillero 8.887
Patrickios 4.685
IPC_peptide 8.668
IPC2_peptide 7.468
IPC2.peptide.svr19 7.776
Protein with the highest isoelectric point:
>tr|A0A1W6R5F0|A0A1W6R5F0_9VIRU Putative coat protein OS=Zostera marina amalgavirus 1 OX=1985202 PE=4 SV=1
MM1 pKa = 7.86 AEE3 pKa = 4.71 DD4 pKa = 4.62 LRR6 pKa = 11.84 SQQTLDD12 pKa = 4.41 DD13 pKa = 4.27 RR14 pKa = 11.84 DD15 pKa = 3.9 NLKK18 pKa = 10.9 LLTDD22 pKa = 4.08 AFKK25 pKa = 10.6 AYY27 pKa = 9.65 PEE29 pKa = 4.95 GGLSVVDD36 pKa = 4.12 VSLEE40 pKa = 4.15 GIAACNYY47 pKa = 6.33 TVSRR51 pKa = 11.84 AVKK54 pKa = 9.85 VMKK57 pKa = 10.04 ILKK60 pKa = 9.25 PLTKK64 pKa = 10.03 NEE66 pKa = 3.86 HH67 pKa = 6.22 LIKK70 pKa = 10.47 LFHH73 pKa = 6.38 YY74 pKa = 10.79 ANDD77 pKa = 3.25 VSNISSILPMTLEE90 pKa = 4.49 TNFKK94 pKa = 8.5 FCKK97 pKa = 9.62 WLTSPVAKK105 pKa = 10.29 KK106 pKa = 10.45 KK107 pKa = 10.21 IAALQNADD115 pKa = 3.56 RR116 pKa = 11.84 LRR118 pKa = 11.84 RR119 pKa = 11.84 RR120 pKa = 11.84 GSDD123 pKa = 3.08 VVTPEE128 pKa = 3.75 EE129 pKa = 3.76 AAMIALLEE137 pKa = 4.21 SAQADD142 pKa = 3.97 RR143 pKa = 11.84 TTEE146 pKa = 3.86 YY147 pKa = 10.44 SKK149 pKa = 11.34 ARR151 pKa = 11.84 ARR153 pKa = 11.84 YY154 pKa = 9.35 DD155 pKa = 3.14 IDD157 pKa = 3.37 VAKK160 pKa = 10.4 YY161 pKa = 9.17 KK162 pKa = 10.79 KK163 pKa = 10.3 KK164 pKa = 8.77 IAKK167 pKa = 7.74 RR168 pKa = 11.84 TRR170 pKa = 11.84 QLEE173 pKa = 3.88 EE174 pKa = 5.19 DD175 pKa = 3.74 LDD177 pKa = 4.27 KK178 pKa = 11.69 VQASYY183 pKa = 10.83 PGLQLIEE190 pKa = 4.79 RR191 pKa = 11.84 PDD193 pKa = 3.62 EE194 pKa = 4.36 HH195 pKa = 9.05 SVMASAWHH203 pKa = 6.72 RR204 pKa = 11.84 YY205 pKa = 7.58 VDD207 pKa = 4.67 FCTSNNFEE215 pKa = 4.29 VPQKK219 pKa = 11.16 NDD221 pKa = 2.85 GNLARR226 pKa = 11.84 AYY228 pKa = 9.69 KK229 pKa = 9.92 QFEE232 pKa = 4.26 RR233 pKa = 11.84 EE234 pKa = 3.8 LTLEE238 pKa = 4.29 IKK240 pKa = 9.21 NTACQKK246 pKa = 10.61 PEE248 pKa = 3.65 VRR250 pKa = 11.84 DD251 pKa = 3.66 YY252 pKa = 11.57 LLQYY256 pKa = 10.49 CKK258 pKa = 10.6 EE259 pKa = 4.05 KK260 pKa = 11.29 VKK262 pKa = 10.93 GFVPNSKK269 pKa = 8.94 TKK271 pKa = 8.86 EE272 pKa = 3.87 LKK274 pKa = 10.89 LIGDD278 pKa = 3.96 TMHH281 pKa = 7.02 ARR283 pKa = 11.84 LEE285 pKa = 4.54 TYY287 pKa = 10.11 FLRR290 pKa = 11.84 IPLEE294 pKa = 3.79 RR295 pKa = 11.84 RR296 pKa = 11.84 GRR298 pKa = 11.84 LLDD301 pKa = 3.73 QIPVGRR307 pKa = 11.84 LTRR310 pKa = 11.84 KK311 pKa = 10.23 GKK313 pKa = 6.49 MTPNIPLSQIFSTPALGMTAVHH335 pKa = 6.04 GTKK338 pKa = 8.39 PTPPIQRR345 pKa = 11.84 NLEE348 pKa = 4.04 MTLLPMNLLKK358 pKa = 10.37 TNLRR362 pKa = 11.84 VQLLTEE368 pKa = 4.2 HH369 pKa = 5.92 VRR371 pKa = 11.84 GGVRR375 pKa = 11.84 QISIARR381 pKa = 11.84 SKK383 pKa = 10.1 WEE385 pKa = 3.86 AGVRR389 pKa = 11.84 RR390 pKa = 11.84 IIGGGEE396 pKa = 3.5 MRR398 pKa = 11.84 GWEE401 pKa = 4.42 KK402 pKa = 11.24 DD403 pKa = 3.2 SALYY407 pKa = 10.43 RR408 pKa = 11.84 GGGNLHH414 pKa = 6.71 DD415 pKa = 6.08 AIRR418 pKa = 11.84 LLATGRR424 pKa = 11.84 QDD426 pKa = 3.31 PPGSFLFEE434 pKa = 4.18 HH435 pKa = 6.93 FSLEE439 pKa = 4.04 TARR442 pKa = 11.84 EE443 pKa = 3.86 ILLLPCNLEE452 pKa = 4.14 VPDD455 pKa = 4.27 GPKK458 pKa = 10.07 AVRR461 pKa = 11.84 MKK463 pKa = 10.91 NFNEE467 pKa = 4.48 DD468 pKa = 2.94 ATAGPVLRR476 pKa = 11.84 ALRR479 pKa = 11.84 CKK481 pKa = 10.31 SKK483 pKa = 11.09 YY484 pKa = 9.35 GLKK487 pKa = 10.48 EE488 pKa = 3.64 GLEE491 pKa = 4.23 RR492 pKa = 11.84 IAWDD496 pKa = 3.67 LYY498 pKa = 11.48 DD499 pKa = 3.92 RR500 pKa = 11.84 VGDD503 pKa = 3.65 GKK505 pKa = 10.63 LRR507 pKa = 11.84 RR508 pKa = 11.84 WQLPPLLARR517 pKa = 11.84 IGFRR521 pKa = 11.84 SKK523 pKa = 10.74 LVEE526 pKa = 3.83 QEE528 pKa = 4.1 SAIKK532 pKa = 10.25 KK533 pKa = 9.45 IFSGQPIGRR542 pKa = 11.84 AVMMLDD548 pKa = 4.01 AMEE551 pKa = 4.57 QPFSSPLYY559 pKa = 10.05 NALCDD564 pKa = 3.81 VVSKK568 pKa = 10.59 LNRR571 pKa = 11.84 VPEE574 pKa = 4.38 SGWRR578 pKa = 11.84 NMVVRR583 pKa = 11.84 ASSDD587 pKa = 2.51 WSRR590 pKa = 11.84 FWGDD594 pKa = 2.93 IRR596 pKa = 11.84 NSCAIVEE603 pKa = 4.32 LDD605 pKa = 2.84 WSKK608 pKa = 11.02 FDD610 pKa = 4.12 RR611 pKa = 11.84 EE612 pKa = 4.14 RR613 pKa = 11.84 PKK615 pKa = 11.03 EE616 pKa = 4.32 DD617 pKa = 2.98 IEE619 pKa = 4.16 FVINIFVSCFLPKK632 pKa = 10.04 NRR634 pKa = 11.84 RR635 pKa = 11.84 EE636 pKa = 3.88 RR637 pKa = 11.84 RR638 pKa = 11.84 LLSAYY643 pKa = 9.69 KK644 pKa = 10.68 YY645 pKa = 9.71 MMRR648 pKa = 11.84 KK649 pKa = 9.51 ALIDD653 pKa = 3.74 KK654 pKa = 10.73 VIMLDD659 pKa = 3.53 DD660 pKa = 4.38 GCAFTYY666 pKa = 10.8 DD667 pKa = 3.34 GMIPSGSLWTGLLGTALNILYY688 pKa = 10.19 INAAVRR694 pKa = 11.84 RR695 pKa = 11.84 LGIPSDD701 pKa = 3.7 EE702 pKa = 4.15 FVPYY706 pKa = 10.42 CAGDD710 pKa = 3.79 DD711 pKa = 3.57 NLTVFKK717 pKa = 10.63 YY718 pKa = 9.06 PQRR721 pKa = 11.84 ATRR724 pKa = 11.84 LGKK727 pKa = 9.78 IRR729 pKa = 11.84 GMLNEE734 pKa = 4.34 MFRR737 pKa = 11.84 AGIDD741 pKa = 3.39 PEE743 pKa = 4.75 DD744 pKa = 4.96 FIIHH748 pKa = 5.99 YY749 pKa = 9.89 PPFHH753 pKa = 5.4 VTKK756 pKa = 10.5 VQAKK760 pKa = 8.74 FPAGFDD766 pKa = 3.73 FTHH769 pKa = 6.37 GTSKK773 pKa = 10.72 FLDD776 pKa = 3.37 QCQWIPLKK784 pKa = 11.25 GNIHH788 pKa = 5.99 VDD790 pKa = 3.03 HH791 pKa = 6.99 SEE793 pKa = 4.12 GLSHH797 pKa = 7.11 RR798 pKa = 11.84 WNYY801 pKa = 8.62 VFKK804 pKa = 10.94 GKK806 pKa = 9.64 PKK808 pKa = 10.08 FLANYY813 pKa = 9.2 FMEE816 pKa = 5.62 DD817 pKa = 3.09 GRR819 pKa = 11.84 SIRR822 pKa = 11.84 PAHH825 pKa = 6.99 DD826 pKa = 3.57 NLEE829 pKa = 4.12 KK830 pKa = 10.79 LLYY833 pKa = 9.45 PEE835 pKa = 5.23 NVQQKK840 pKa = 9.77 IEE842 pKa = 4.26 DD843 pKa = 3.69 YY844 pKa = 9.31 EE845 pKa = 4.23 AAVLSMAVDD854 pKa = 4.03 NPWNSHH860 pKa = 5.81 NINHH864 pKa = 6.62 LMQRR868 pKa = 11.84 FCIIQQIKK876 pKa = 8.86 RR877 pKa = 11.84 QAVHH881 pKa = 7.18 PLTAGDD887 pKa = 4.06 VLFCAKK893 pKa = 9.85 FQSISEE899 pKa = 4.23 NMWMFPTVGAWRR911 pKa = 11.84 RR912 pKa = 11.84 QLLNVRR918 pKa = 11.84 MEE920 pKa = 5.35 DD921 pKa = 3.49 IEE923 pKa = 4.21 EE924 pKa = 4.22 LKK926 pKa = 9.61 PTVEE930 pKa = 4.11 AFSEE934 pKa = 4.7 FVRR937 pKa = 11.84 GVTSLYY943 pKa = 10.86 SRR945 pKa = 11.84 ASTGGLDD952 pKa = 2.73 SWQFMEE958 pKa = 4.18 ILRR961 pKa = 11.84 GEE963 pKa = 4.07 RR964 pKa = 11.84 DD965 pKa = 3.48 AGSGQYY971 pKa = 10.92 GSDD974 pKa = 3.09 IEE976 pKa = 4.27 AWCAFLNRR984 pKa = 11.84 NGLTRR989 pKa = 11.84 SLRR992 pKa = 11.84 PAKK995 pKa = 10.15 RR996 pKa = 11.84 YY997 pKa = 9.64 RR998 pKa = 11.84 KK999 pKa = 9.29 EE1000 pKa = 3.62 KK1001 pKa = 9.1 QTYY1004 pKa = 8.42 QSAEE1008 pKa = 3.99 AGDD1011 pKa = 4.46 KK1012 pKa = 10.82 ILVSLAAIYY1021 pKa = 7.63 ATSDD1025 pKa = 2.92 YY1026 pKa = 11.23 DD1027 pKa = 3.96 LRR1029 pKa = 11.84 EE1030 pKa = 4.24 DD1031 pKa = 4.02 RR1032 pKa = 11.84 DD1033 pKa = 3.54 LGFIIRR1039 pKa = 11.84 ISNEE1043 pKa = 3.24 LRR1045 pKa = 11.84 KK1046 pKa = 9.61 QLLL1049 pKa = 3.44
Molecular weight: 119.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.72
IPC2_protein 8.595
IPC_protein 8.463
Toseland 9.165
ProMoST 9.019
Dawson 9.487
Bjellqvist 9.297
Wikipedia 9.648
Rodwell 9.721
Grimsley 9.545
Solomon 9.516
Lehninger 9.472
Nozaki 9.443
DTASelect 9.209
Thurlkill 9.355
EMBOSS 9.648
Sillero 9.502
Patrickios 4.927
IPC_peptide 9.516
IPC2_peptide 7.951
IPC2.peptide.svr19 7.719
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1431
382
1049
715.5
81.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.036 ± 0.904
1.607 ± 0.123
6.499 ± 1.03
6.499 ± 0.456
3.704 ± 0.739
5.451 ± 0.979
1.817 ± 0.009
5.101 ± 0.931
7.407 ± 1.106
9.853 ± 1.097
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.376 ± 0.585
4.333 ± 0.064
4.682 ± 0.127
3.704 ± 0.122
8.036 ± 0.1
5.94 ± 0.188
4.822 ± 0.801
5.381 ± 0.08
1.468 ± 0.374
3.284 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here