Oceanihabitans sediminis
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2601 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A368P1M9|A0A368P1M9_9FLAO DUF11 domain-containing protein OS=Oceanihabitans sediminis OX=1812012 GN=DU428_12705 PE=4 SV=1
MM1 pKa = 7.63 NLRR4 pKa = 11.84 KK5 pKa = 9.65 IIFTIFTVFLVLVSCKK21 pKa = 10.59 DD22 pKa = 3.61 DD23 pKa = 3.99 DD24 pKa = 4.69 DD25 pKa = 4.32 KK26 pKa = 12.22 VEE28 pKa = 4.24 IVPPRR33 pKa = 11.84 DD34 pKa = 3.16 RR35 pKa = 11.84 TEE37 pKa = 4.11 VYY39 pKa = 10.85 AEE41 pKa = 4.03 DD42 pKa = 3.95 LEE44 pKa = 4.6 EE45 pKa = 6.08 IEE47 pKa = 5.14 TFLQTHH53 pKa = 5.93 YY54 pKa = 11.02 FNYY57 pKa = 10.67 DD58 pKa = 2.96 EE59 pKa = 5.91 FDD61 pKa = 3.65 FSDD64 pKa = 4.7 PYY66 pKa = 11.57 SPANDD71 pKa = 3.19 NYY73 pKa = 10.45 EE74 pKa = 3.93 IVFGKK79 pKa = 10.23 IEE81 pKa = 5.12 DD82 pKa = 4.83 DD83 pKa = 3.64 PTKK86 pKa = 10.57 TPIMDD91 pKa = 3.92 MLDD94 pKa = 3.57 QEE96 pKa = 4.66 NGPLKK101 pKa = 10.46 YY102 pKa = 10.27 KK103 pKa = 10.26 EE104 pKa = 4.07 VQDD107 pKa = 3.95 PVEE110 pKa = 3.98 EE111 pKa = 4.2 SLVYY115 pKa = 10.08 RR116 pKa = 11.84 LYY118 pKa = 11.31 YY119 pKa = 10.35 LDD121 pKa = 3.27 IRR123 pKa = 11.84 EE124 pKa = 4.34 GQGYY128 pKa = 8.61 EE129 pKa = 4.16 LNSIDD134 pKa = 5.55 KK135 pKa = 10.92 VYY137 pKa = 11.14 LNYY140 pKa = 11.0 DD141 pKa = 3.31 GVLSDD146 pKa = 4.11 NEE148 pKa = 4.48 SFEE151 pKa = 4.58 STVNPINLEE160 pKa = 3.97 LTYY163 pKa = 9.59 LTTGGVVTGLRR174 pKa = 11.84 EE175 pKa = 3.81 ALLEE179 pKa = 4.43 FKK181 pKa = 9.93 TATNFTEE188 pKa = 4.65 NGDD191 pKa = 3.72 GTTTYY196 pKa = 10.14 HH197 pKa = 5.3 NHH199 pKa = 6.89 GIGAAFVPSGLGYY212 pKa = 10.25 FEE214 pKa = 5.86 IYY216 pKa = 9.95 QNNIPNYY223 pKa = 9.61 SPLIFKK229 pKa = 10.18 FYY231 pKa = 10.58 LYY233 pKa = 10.97 DD234 pKa = 3.62 RR235 pKa = 11.84 TLLDD239 pKa = 3.78 HH240 pKa = 7.51 DD241 pKa = 4.87 KK242 pKa = 11.52 DD243 pKa = 4.4 GIPTILEE250 pKa = 4.38 DD251 pKa = 4.7 LDD253 pKa = 4.53 GDD255 pKa = 3.87 EE256 pKa = 6.12 NYY258 pKa = 10.46 FNDD261 pKa = 4.31 DD262 pKa = 3.29 TDD264 pKa = 5.04 GDD266 pKa = 4.13 GAPNFFDD273 pKa = 5.58 KK274 pKa = 11.13 DD275 pKa = 3.71 DD276 pKa = 5.28 DD277 pKa = 4.9 GDD279 pKa = 4.53 GIPTKK284 pKa = 10.89 DD285 pKa = 3.49 EE286 pKa = 5.13 IILKK290 pKa = 8.14 TYY292 pKa = 9.91 PEE294 pKa = 3.97 EE295 pKa = 4.94 GMADD299 pKa = 4.16 FLTKK303 pKa = 10.34 EE304 pKa = 3.97 DD305 pKa = 3.43 AQAYY309 pKa = 9.34 FDD311 pKa = 4.26 TNAADD316 pKa = 3.67 DD317 pKa = 3.97 EE318 pKa = 4.75 VFIEE322 pKa = 3.66 IRR324 pKa = 11.84 YY325 pKa = 8.8 NNSEE329 pKa = 3.64 ATYY332 pKa = 9.18 TLKK335 pKa = 10.19 TFIAPDD341 pKa = 3.48 SNGNGKK347 pKa = 8.92 PDD349 pKa = 3.92 YY350 pKa = 11.19 LDD352 pKa = 2.62 ITYY355 pKa = 9.46 PVQQ358 pKa = 3.54
Molecular weight: 40.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.834
IPC_protein 3.859
Toseland 3.643
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.681
Grimsley 3.541
Solomon 3.846
Lehninger 3.795
Nozaki 3.948
DTASelect 4.19
Thurlkill 3.681
EMBOSS 3.783
Sillero 3.973
Patrickios 1.418
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.871
Protein with the highest isoelectric point:
>tr|A0A368P3K2|A0A368P3K2_9FLAO D-cysteine desulfhydrase family protein OS=Oceanihabitans sediminis OX=1812012 GN=DU428_12185 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 10.15 RR11 pKa = 11.84 KK12 pKa = 9.6 RR13 pKa = 11.84 KK14 pKa = 8.29 NKK16 pKa = 9.34 HH17 pKa = 4.03 GFRR20 pKa = 11.84 EE21 pKa = 4.2 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.09 GRR40 pKa = 11.84 KK41 pKa = 7.97 KK42 pKa = 10.52 LSVSSEE48 pKa = 3.87 PRR50 pKa = 11.84 HH51 pKa = 5.92 KK52 pKa = 10.61 KK53 pKa = 9.84
Molecular weight: 6.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.457
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.296
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.018
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.041
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2601
0
2601
861170
38
2395
331.1
37.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.471 ± 0.049
0.758 ± 0.016
5.51 ± 0.042
6.932 ± 0.048
5.188 ± 0.044
6.071 ± 0.053
1.823 ± 0.023
8.047 ± 0.04
8.091 ± 0.07
9.261 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.096 ± 0.022
6.242 ± 0.052
3.264 ± 0.029
3.369 ± 0.029
3.279 ± 0.031
6.368 ± 0.042
5.893 ± 0.067
6.302 ± 0.038
0.941 ± 0.016
4.094 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here