Amino acid dipepetide frequency for Escherichia phage mckay

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.465AlaAla: 12.465 ± 1.55
0.885AlaCys: 0.885 ± 0.293
6.933AlaAsp: 6.933 ± 0.821
6.122AlaGlu: 6.122 ± 0.677
3.467AlaPhe: 3.467 ± 0.521
8.261AlaGly: 8.261 ± 0.879
1.844AlaHis: 1.844 ± 0.31
6.269AlaIle: 6.269 ± 0.646
6.859AlaLys: 6.859 ± 0.946
7.449AlaLeu: 7.449 ± 0.726
3.024AlaMet: 3.024 ± 0.44
3.614AlaAsn: 3.614 ± 0.754
2.95AlaPro: 2.95 ± 0.475
4.647AlaGln: 4.647 ± 0.82
6.269AlaArg: 6.269 ± 0.606
4.942AlaSer: 4.942 ± 0.49
4.942AlaThr: 4.942 ± 0.66
7.376AlaVal: 7.376 ± 0.737
1.844AlaTrp: 1.844 ± 0.33
3.024AlaTyr: 3.024 ± 0.459
0.0AlaXaa: 0.0 ± 0.0
Cys
1.254CysAla: 1.254 ± 0.355
0.295CysCys: 0.295 ± 0.121
0.738CysAsp: 0.738 ± 0.252
0.738CysGlu: 0.738 ± 0.235
0.516CysPhe: 0.516 ± 0.206
1.106CysGly: 1.106 ± 0.364
0.148CysHis: 0.148 ± 0.103
0.59CysIle: 0.59 ± 0.206
0.885CysLys: 0.885 ± 0.243
0.885CysLeu: 0.885 ± 0.254
0.443CysMet: 0.443 ± 0.156
0.738CysAsn: 0.738 ± 0.226
0.295CysPro: 0.295 ± 0.137
0.295CysGln: 0.295 ± 0.124
0.811CysArg: 0.811 ± 0.216
0.516CysSer: 0.516 ± 0.187
0.516CysThr: 0.516 ± 0.19
0.738CysVal: 0.738 ± 0.193
0.295CysTrp: 0.295 ± 0.165
0.516CysTyr: 0.516 ± 0.162
0.0CysXaa: 0.0 ± 0.0
Asp
6.712AspAla: 6.712 ± 0.677
0.811AspCys: 0.811 ± 0.217
4.13AspAsp: 4.13 ± 1.029
5.163AspGlu: 5.163 ± 1.041
2.95AspPhe: 2.95 ± 0.395
5.974AspGly: 5.974 ± 0.652
1.328AspHis: 1.328 ± 0.346
2.877AspIle: 2.877 ± 0.392
3.172AspLys: 3.172 ± 0.517
5.458AspLeu: 5.458 ± 0.752
2.065AspMet: 2.065 ± 0.347
2.729AspAsn: 2.729 ± 0.569
3.319AspPro: 3.319 ± 0.461
1.696AspGln: 1.696 ± 0.37
2.655AspArg: 2.655 ± 0.365
2.508AspSer: 2.508 ± 0.441
3.762AspThr: 3.762 ± 0.473
4.942AspVal: 4.942 ± 0.499
1.106AspTrp: 1.106 ± 0.352
1.18AspTyr: 1.18 ± 0.29
0.0AspXaa: 0.0 ± 0.0
Glu
5.753GluAla: 5.753 ± 0.77
0.959GluCys: 0.959 ± 0.305
4.72GluAsp: 4.72 ± 0.778
3.835GluGlu: 3.835 ± 0.715
2.36GluPhe: 2.36 ± 0.443
3.172GluGly: 3.172 ± 0.52
1.328GluHis: 1.328 ± 0.35
4.942GluIle: 4.942 ± 0.496
3.54GluLys: 3.54 ± 0.698
4.794GluLeu: 4.794 ± 0.52
1.696GluMet: 1.696 ± 0.359
2.655GluAsn: 2.655 ± 0.466
1.401GluPro: 1.401 ± 0.401
2.508GluGln: 2.508 ± 0.413
4.057GluArg: 4.057 ± 0.564
2.95GluSer: 2.95 ± 0.473
2.286GluThr: 2.286 ± 0.454
4.278GluVal: 4.278 ± 0.419
0.959GluTrp: 0.959 ± 0.238
3.098GluTyr: 3.098 ± 0.493
0.0GluXaa: 0.0 ± 0.0
Phe
3.614PheAla: 3.614 ± 0.46
0.369PheCys: 0.369 ± 0.131
2.877PheAsp: 2.877 ± 0.516
2.139PheGlu: 2.139 ± 0.347
1.033PhePhe: 1.033 ± 0.288
3.54PheGly: 3.54 ± 0.493
0.664PheHis: 0.664 ± 0.238
2.213PheIle: 2.213 ± 0.359
1.844PheLys: 1.844 ± 0.331
1.918PheLeu: 1.918 ± 0.346
0.664PheMet: 0.664 ± 0.233
1.475PheAsn: 1.475 ± 0.326
1.475PhePro: 1.475 ± 0.344
1.475PheGln: 1.475 ± 0.266
2.729PheArg: 2.729 ± 0.354
1.77PheSer: 1.77 ± 0.314
2.36PheThr: 2.36 ± 0.4
2.877PheVal: 2.877 ± 0.576
0.59PheTrp: 0.59 ± 0.224
1.033PheTyr: 1.033 ± 0.26
0.0PheXaa: 0.0 ± 0.0
Gly
6.491GlyAla: 6.491 ± 0.67
1.106GlyCys: 1.106 ± 0.27
4.647GlyAsp: 4.647 ± 0.592
4.942GlyGlu: 4.942 ± 0.539
3.245GlyPhe: 3.245 ± 0.396
6.343GlyGly: 6.343 ± 0.89
1.696GlyHis: 1.696 ± 0.475
4.057GlyIle: 4.057 ± 0.485
6.417GlyLys: 6.417 ± 0.826
6.122GlyLeu: 6.122 ± 0.66
2.286GlyMet: 2.286 ± 0.449
4.13GlyAsn: 4.13 ± 0.815
2.065GlyPro: 2.065 ± 0.34
2.213GlyGln: 2.213 ± 0.353
4.573GlyArg: 4.573 ± 0.579
3.54GlySer: 3.54 ± 0.516
4.057GlyThr: 4.057 ± 0.633
6.122GlyVal: 6.122 ± 0.761
0.959GlyTrp: 0.959 ± 0.297
2.139GlyTyr: 2.139 ± 0.373
0.0GlyXaa: 0.0 ± 0.0
His
1.106HisAla: 1.106 ± 0.268
0.369HisCys: 0.369 ± 0.161
1.328HisAsp: 1.328 ± 0.3
0.811HisGlu: 0.811 ± 0.206
0.369HisPhe: 0.369 ± 0.172
1.106HisGly: 1.106 ± 0.289
0.664HisHis: 0.664 ± 0.248
1.18HisIle: 1.18 ± 0.281
1.475HisLys: 1.475 ± 0.328
1.328HisLeu: 1.328 ± 0.319
0.295HisMet: 0.295 ± 0.145
0.664HisAsn: 0.664 ± 0.179
1.401HisPro: 1.401 ± 0.303
0.885HisGln: 0.885 ± 0.312
0.959HisArg: 0.959 ± 0.24
0.59HisSer: 0.59 ± 0.184
1.475HisThr: 1.475 ± 0.42
0.885HisVal: 0.885 ± 0.198
0.443HisTrp: 0.443 ± 0.152
1.033HisTyr: 1.033 ± 0.305
0.0HisXaa: 0.0 ± 0.0
Ile
5.827IleAla: 5.827 ± 0.454
0.664IleCys: 0.664 ± 0.232
3.909IleAsp: 3.909 ± 0.453
3.319IleGlu: 3.319 ± 0.42
1.623IlePhe: 1.623 ± 0.321
3.54IleGly: 3.54 ± 0.547
0.811IleHis: 0.811 ± 0.211
2.729IleIle: 2.729 ± 0.412
3.614IleLys: 3.614 ± 0.46
3.098IleLeu: 3.098 ± 0.47
1.623IleMet: 1.623 ± 0.307
3.245IleAsn: 3.245 ± 0.497
2.434IlePro: 2.434 ± 0.492
1.844IleGln: 1.844 ± 0.487
3.245IleArg: 3.245 ± 0.451
2.36IleSer: 2.36 ± 0.482
3.835IleThr: 3.835 ± 0.845
3.688IleVal: 3.688 ± 0.404
0.959IleTrp: 0.959 ± 0.279
1.549IleTyr: 1.549 ± 0.347
0.0IleXaa: 0.0 ± 0.0
Lys
7.449LysAla: 7.449 ± 0.878
0.885LysCys: 0.885 ± 0.265
2.877LysAsp: 2.877 ± 0.398
3.762LysGlu: 3.762 ± 0.607
1.918LysPhe: 1.918 ± 0.279
3.983LysGly: 3.983 ± 0.466
1.254LysHis: 1.254 ± 0.315
2.508LysIle: 2.508 ± 0.493
2.95LysLys: 2.95 ± 0.507
4.794LysLeu: 4.794 ± 0.663
2.286LysMet: 2.286 ± 0.366
2.286LysAsn: 2.286 ± 0.446
3.098LysPro: 3.098 ± 0.556
2.95LysGln: 2.95 ± 0.55
4.278LysArg: 4.278 ± 0.586
3.688LysSer: 3.688 ± 0.735
4.057LysThr: 4.057 ± 0.41
3.983LysVal: 3.983 ± 0.462
1.18LysTrp: 1.18 ± 0.28
1.328LysTyr: 1.328 ± 0.275
0.0LysXaa: 0.0 ± 0.0
Leu
6.712LeuAla: 6.712 ± 0.725
0.811LeuCys: 0.811 ± 0.243
4.647LeuAsp: 4.647 ± 0.495
3.688LeuGlu: 3.688 ± 0.479
3.024LeuPhe: 3.024 ± 0.378
4.942LeuGly: 4.942 ± 0.508
1.328LeuHis: 1.328 ± 0.35
4.425LeuIle: 4.425 ± 0.496
4.425LeuLys: 4.425 ± 0.566
5.237LeuLeu: 5.237 ± 0.621
2.286LeuMet: 2.286 ± 0.406
3.393LeuAsn: 3.393 ± 0.438
3.393LeuPro: 3.393 ± 0.653
3.245LeuGln: 3.245 ± 0.425
4.942LeuArg: 4.942 ± 0.566
4.647LeuSer: 4.647 ± 0.462
5.974LeuThr: 5.974 ± 0.675
5.089LeuVal: 5.089 ± 0.674
0.516LeuTrp: 0.516 ± 0.168
2.213LeuTyr: 2.213 ± 0.348
0.0LeuXaa: 0.0 ± 0.0
Met
3.024MetAla: 3.024 ± 0.498
0.295MetCys: 0.295 ± 0.137
1.18MetAsp: 1.18 ± 0.3
1.475MetGlu: 1.475 ± 0.322
1.033MetPhe: 1.033 ± 0.279
1.77MetGly: 1.77 ± 0.556
0.59MetHis: 0.59 ± 0.185
1.844MetIle: 1.844 ± 0.357
1.918MetLys: 1.918 ± 0.367
2.434MetLeu: 2.434 ± 0.401
0.443MetMet: 0.443 ± 0.201
1.254MetAsn: 1.254 ± 0.344
1.033MetPro: 1.033 ± 0.284
1.106MetGln: 1.106 ± 0.202
1.475MetArg: 1.475 ± 0.367
1.844MetSer: 1.844 ± 0.359
1.77MetThr: 1.77 ± 0.396
1.844MetVal: 1.844 ± 0.393
0.369MetTrp: 0.369 ± 0.138
0.959MetTyr: 0.959 ± 0.258
0.0MetXaa: 0.0 ± 0.0
Asn
5.163AsnAla: 5.163 ± 0.653
0.369AsnCys: 0.369 ± 0.18
2.508AsnAsp: 2.508 ± 0.384
2.434AsnGlu: 2.434 ± 0.543
1.401AsnPhe: 1.401 ± 0.265
4.499AsnGly: 4.499 ± 0.592
1.033AsnHis: 1.033 ± 0.305
1.844AsnIle: 1.844 ± 0.292
2.139AsnLys: 2.139 ± 0.419
2.508AsnLeu: 2.508 ± 0.387
1.254AsnMet: 1.254 ± 0.295
1.623AsnAsn: 1.623 ± 0.438
1.918AsnPro: 1.918 ± 0.37
1.696AsnGln: 1.696 ± 0.3
2.065AsnArg: 2.065 ± 0.311
1.254AsnSer: 1.254 ± 0.31
2.655AsnThr: 2.655 ± 0.357
3.245AsnVal: 3.245 ± 0.488
0.811AsnTrp: 0.811 ± 0.248
1.844AsnTyr: 1.844 ± 0.405
0.0AsnXaa: 0.0 ± 0.0
Pro
4.057ProAla: 4.057 ± 0.443
0.443ProCys: 0.443 ± 0.182
3.614ProAsp: 3.614 ± 0.569
2.213ProGlu: 2.213 ± 0.452
1.18ProPhe: 1.18 ± 0.271
3.688ProGly: 3.688 ± 0.64
0.664ProHis: 0.664 ± 0.242
2.139ProIle: 2.139 ± 0.313
2.065ProLys: 2.065 ± 0.399
3.393ProLeu: 3.393 ± 0.666
0.369ProMet: 0.369 ± 0.175
1.18ProAsn: 1.18 ± 0.24
1.991ProPro: 1.991 ± 0.476
1.254ProGln: 1.254 ± 0.291
2.729ProArg: 2.729 ± 0.459
1.844ProSer: 1.844 ± 0.309
2.582ProThr: 2.582 ± 0.511
2.95ProVal: 2.95 ± 0.385
0.443ProTrp: 0.443 ± 0.196
1.254ProTyr: 1.254 ± 0.368
0.0ProXaa: 0.0 ± 0.0
Gln
3.393GlnAla: 3.393 ± 0.479
0.295GlnCys: 0.295 ± 0.156
1.696GlnAsp: 1.696 ± 0.299
1.549GlnGlu: 1.549 ± 0.343
1.401GlnPhe: 1.401 ± 0.382
2.286GlnGly: 2.286 ± 0.361
0.885GlnHis: 0.885 ± 0.23
3.098GlnIle: 3.098 ± 0.566
1.844GlnLys: 1.844 ± 0.359
3.909GlnLeu: 3.909 ± 0.49
1.328GlnMet: 1.328 ± 0.298
0.885GlnAsn: 0.885 ± 0.268
1.991GlnPro: 1.991 ± 0.333
1.401GlnGln: 1.401 ± 0.418
2.434GlnArg: 2.434 ± 0.439
2.065GlnSer: 2.065 ± 0.415
2.582GlnThr: 2.582 ± 0.467
2.95GlnVal: 2.95 ± 0.407
0.885GlnTrp: 0.885 ± 0.272
1.106GlnTyr: 1.106 ± 0.258
0.0GlnXaa: 0.0 ± 0.0
Arg
6.048ArgAla: 6.048 ± 0.787
0.811ArgCys: 0.811 ± 0.225
3.172ArgAsp: 3.172 ± 0.562
3.393ArgGlu: 3.393 ± 0.523
2.877ArgPhe: 2.877 ± 0.45
3.762ArgGly: 3.762 ± 0.541
1.254ArgHis: 1.254 ± 0.275
3.467ArgIle: 3.467 ± 0.667
4.868ArgLys: 4.868 ± 0.771
4.942ArgLeu: 4.942 ± 0.537
1.549ArgMet: 1.549 ± 0.364
2.803ArgAsn: 2.803 ± 0.461
2.434ArgPro: 2.434 ± 0.401
2.286ArgGln: 2.286 ± 0.353
4.647ArgArg: 4.647 ± 0.656
2.36ArgSer: 2.36 ± 0.423
3.172ArgThr: 3.172 ± 0.407
5.237ArgVal: 5.237 ± 0.694
0.885ArgTrp: 0.885 ± 0.252
1.623ArgTyr: 1.623 ± 0.333
0.0ArgXaa: 0.0 ± 0.0
Ser
4.057SerAla: 4.057 ± 0.579
0.295SerCys: 0.295 ± 0.123
2.729SerAsp: 2.729 ± 0.448
3.762SerGlu: 3.762 ± 0.518
1.844SerPhe: 1.844 ± 0.359
5.974SerGly: 5.974 ± 0.719
0.738SerHis: 0.738 ± 0.195
2.286SerIle: 2.286 ± 0.416
2.729SerLys: 2.729 ± 0.568
3.983SerLeu: 3.983 ± 0.474
0.885SerMet: 0.885 ± 0.264
2.286SerAsn: 2.286 ± 0.384
1.328SerPro: 1.328 ± 0.239
1.623SerGln: 1.623 ± 0.298
1.918SerArg: 1.918 ± 0.335
2.36SerSer: 2.36 ± 0.32
3.245SerThr: 3.245 ± 0.482
3.688SerVal: 3.688 ± 0.5
1.033SerTrp: 1.033 ± 0.281
1.328SerTyr: 1.328 ± 0.298
0.0SerXaa: 0.0 ± 0.0
Thr
6.343ThrAla: 6.343 ± 0.72
0.811ThrCys: 0.811 ± 0.25
3.909ThrAsp: 3.909 ± 0.561
3.983ThrGlu: 3.983 ± 0.544
2.729ThrPhe: 2.729 ± 0.483
4.72ThrGly: 4.72 ± 0.588
0.443ThrHis: 0.443 ± 0.182
2.286ThrIle: 2.286 ± 0.422
3.393ThrLys: 3.393 ± 0.577
5.015ThrLeu: 5.015 ± 0.628
1.991ThrMet: 1.991 ± 0.375
2.803ThrAsn: 2.803 ± 0.565
2.877ThrPro: 2.877 ± 0.446
2.508ThrGln: 2.508 ± 0.418
3.245ThrArg: 3.245 ± 0.419
3.467ThrSer: 3.467 ± 0.665
2.803ThrThr: 2.803 ± 0.474
3.835ThrVal: 3.835 ± 0.547
1.18ThrTrp: 1.18 ± 0.277
1.401ThrTyr: 1.401 ± 0.4
0.0ThrXaa: 0.0 ± 0.0
Val
7.523ValAla: 7.523 ± 0.619
0.738ValCys: 0.738 ± 0.23
5.458ValAsp: 5.458 ± 0.691
5.606ValGlu: 5.606 ± 0.667
2.36ValPhe: 2.36 ± 0.396
4.942ValGly: 4.942 ± 0.778
0.959ValHis: 0.959 ± 0.218
3.835ValIle: 3.835 ± 0.572
4.499ValLys: 4.499 ± 0.621
5.015ValLeu: 5.015 ± 0.42
1.623ValMet: 1.623 ± 0.33
2.95ValAsn: 2.95 ± 0.468
2.36ValPro: 2.36 ± 0.405
2.95ValGln: 2.95 ± 0.364
5.384ValArg: 5.384 ± 0.491
3.245ValSer: 3.245 ± 0.468
4.13ValThr: 4.13 ± 0.605
6.196ValVal: 6.196 ± 0.711
0.738ValTrp: 0.738 ± 0.265
1.918ValTyr: 1.918 ± 0.428
0.0ValXaa: 0.0 ± 0.0
Trp
2.213TrpAla: 2.213 ± 0.555
0.443TrpCys: 0.443 ± 0.174
1.033TrpAsp: 1.033 ± 0.209
0.885TrpGlu: 0.885 ± 0.211
0.59TrpPhe: 0.59 ± 0.214
0.885TrpGly: 0.885 ± 0.268
0.443TrpHis: 0.443 ± 0.164
0.443TrpIle: 0.443 ± 0.181
0.811TrpLys: 0.811 ± 0.241
0.885TrpLeu: 0.885 ± 0.267
0.59TrpMet: 0.59 ± 0.154
0.59TrpAsn: 0.59 ± 0.186
0.811TrpPro: 0.811 ± 0.281
0.369TrpGln: 0.369 ± 0.157
1.401TrpArg: 1.401 ± 0.326
0.811TrpSer: 0.811 ± 0.221
0.738TrpThr: 0.738 ± 0.217
1.475TrpVal: 1.475 ± 0.35
0.443TrpTrp: 0.443 ± 0.156
0.443TrpTyr: 0.443 ± 0.159
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.983TyrAla: 3.983 ± 0.414
0.664TyrCys: 0.664 ± 0.214
2.434TyrAsp: 2.434 ± 0.439
1.475TyrGlu: 1.475 ± 0.406
0.738TyrPhe: 0.738 ± 0.227
2.655TyrGly: 2.655 ± 0.466
0.295TyrHis: 0.295 ± 0.143
0.885TyrIle: 0.885 ± 0.26
2.065TyrLys: 2.065 ± 0.344
1.77TyrLeu: 1.77 ± 0.345
0.959TyrMet: 0.959 ± 0.231
0.959TyrAsn: 0.959 ± 0.283
1.475TyrPro: 1.475 ± 0.421
1.033TyrGln: 1.033 ± 0.313
1.77TyrArg: 1.77 ± 0.369
1.328TyrSer: 1.328 ± 0.3
2.803TyrThr: 2.803 ± 0.437
1.033TyrVal: 1.033 ± 0.248
0.664TyrTrp: 0.664 ± 0.228
1.033TyrTyr: 1.033 ± 0.272
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (13559 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski