Staphylococcus phage SAP3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rockefellervirus; unclassified Rockefellervirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D7INT2|A0A7D7INT2_9CAUD Uncharacterized protein OS=Staphylococcus phage SAP3 OX=2759187 GN=SAP3_62 PE=4 SV=1
MM1 pKa = 8.09AEE3 pKa = 3.97MYY5 pKa = 10.07YY6 pKa = 10.57GYY8 pKa = 10.43EE9 pKa = 3.94GTPYY13 pKa = 10.92LVMNIEE19 pKa = 4.88DD20 pKa = 4.56LNNLPSDD27 pKa = 3.99VTKK30 pKa = 10.3TPPPNGIYY38 pKa = 10.26RR39 pKa = 11.84PFYY42 pKa = 10.28YY43 pKa = 10.41DD44 pKa = 3.55SEE46 pKa = 4.88NDD48 pKa = 3.09TWHH51 pKa = 6.87GSDD54 pKa = 2.96EE55 pKa = 4.76SEE57 pKa = 4.12FLRR60 pKa = 11.84SVEE63 pKa = 4.07EE64 pKa = 4.02EE65 pKa = 4.34KK66 pKa = 10.92EE67 pKa = 4.31VIPITSNDD75 pKa = 3.48EE76 pKa = 4.33VILSLTQKK84 pKa = 10.24VAEE87 pKa = 4.19QEE89 pKa = 4.17LKK91 pKa = 10.1IDD93 pKa = 3.86SLEE96 pKa = 4.04DD97 pKa = 3.72TIAMLLLKK105 pKa = 10.33NAEE108 pKa = 4.49LGDD111 pKa = 4.5DD112 pKa = 3.77NDD114 pKa = 3.99VVV116 pKa = 3.61

Molecular weight: 13.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D7JEM0|A0A7D7JEM0_9CAUD Phage_Mu_F domain-containing protein OS=Staphylococcus phage SAP3 OX=2759187 GN=SAP3_21 PE=4 SV=1
MM1 pKa = 6.81HH2 pKa = 7.02TVLALHH8 pKa = 6.96RR9 pKa = 11.84NGEE12 pKa = 4.2KK13 pKa = 9.25PTITSHH19 pKa = 8.06DD20 pKa = 3.84EE21 pKa = 4.02FQKK24 pKa = 11.37SKK26 pKa = 10.74MEE28 pKa = 4.19DD29 pKa = 2.67AYY31 pKa = 11.34RR32 pKa = 11.84NYY34 pKa = 10.23KK35 pKa = 10.07AEE37 pKa = 4.18RR38 pKa = 11.84EE39 pKa = 4.22AKK41 pKa = 8.98PWLKK45 pKa = 9.31TVPQSVKK52 pKa = 10.18PSRR55 pKa = 11.84AYY57 pKa = 10.85YY58 pKa = 9.83DD59 pKa = 3.25LCRR62 pKa = 11.84FSGVSVKK69 pKa = 10.4QKK71 pKa = 10.2EE72 pKa = 4.47IKK74 pKa = 10.26SYY76 pKa = 9.11PAKK79 pKa = 10.29PKK81 pKa = 9.96EE82 pKa = 4.15KK83 pKa = 9.83KK84 pKa = 10.05LPKK87 pKa = 9.99IPGDD91 pKa = 3.41HH92 pKa = 6.04SRR94 pKa = 11.84EE95 pKa = 4.07FIINGYY101 pKa = 7.94VVSVRR106 pKa = 11.84QLAKK110 pKa = 10.26LLNMRR115 pKa = 11.84YY116 pKa = 9.11EE117 pKa = 4.46VVDD120 pKa = 3.33SRR122 pKa = 11.84LRR124 pKa = 11.84NGFTPEE130 pKa = 3.52EE131 pKa = 3.98LLEE134 pKa = 4.34KK135 pKa = 10.66KK136 pKa = 10.0GVKK139 pKa = 9.44LL140 pKa = 3.86

Molecular weight: 16.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12312

38

1001

205.2

23.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.921 ± 0.424

0.504 ± 0.097

6.457 ± 0.363

7.456 ± 0.518

4.142 ± 0.266

6.1 ± 0.423

1.576 ± 0.159

7.651 ± 0.296

8.699 ± 0.431

7.675 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.51 ± 0.238

6.514 ± 0.321

2.965 ± 0.22

3.801 ± 0.223

4.207 ± 0.264

5.824 ± 0.283

5.945 ± 0.307

6.295 ± 0.279

1.218 ± 0.19

4.54 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski