Lactococcus phage phismq86

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5GZ65|A5GZ65_9CAUD Putative repressor OS=Lactococcus phage phismq86 OX=444474 PE=4 SV=1
MM1 pKa = 8.24DD2 pKa = 4.03EE3 pKa = 4.4QEE5 pKa = 4.71LNSLLICEE13 pKa = 5.17IEE15 pKa = 4.2NQHH18 pKa = 6.03IDD20 pKa = 3.58YY21 pKa = 11.09RR22 pKa = 11.84LGNWNNQVAWVAPLLGLGGYY42 pKa = 7.42EE43 pKa = 3.92KK44 pKa = 10.65NARR47 pKa = 11.84PFDD50 pKa = 3.93HH51 pKa = 6.96AHH53 pKa = 6.01EE54 pKa = 4.77LSHH57 pKa = 7.34ILNHH61 pKa = 6.86DD62 pKa = 3.92DD63 pKa = 4.86YY64 pKa = 11.69RR65 pKa = 11.84GGDD68 pKa = 4.12CDD70 pKa = 3.54TTSPNEE76 pKa = 4.03SRR78 pKa = 11.84AHH80 pKa = 6.33RR81 pKa = 11.84EE82 pKa = 4.04AILLLWDD89 pKa = 3.55MFEE92 pKa = 4.48KK93 pKa = 10.72QGGDD97 pKa = 3.23YY98 pKa = 11.22SHH100 pKa = 7.41FNLFIEE106 pKa = 4.53ITGCPYY112 pKa = 11.09DD113 pKa = 3.48FAYY116 pKa = 10.86SIISKK121 pKa = 9.43EE122 pKa = 3.88FNEE125 pKa = 4.38MYY127 pKa = 10.42EE128 pKa = 4.43AINEE132 pKa = 4.15IFVDD136 pKa = 3.74EE137 pKa = 4.61LNIKK141 pKa = 9.16IKK143 pKa = 10.61KK144 pKa = 7.55EE145 pKa = 3.99QIHH148 pKa = 6.3KK149 pKa = 10.25FAVDD153 pKa = 3.52YY154 pKa = 10.83ISYY157 pKa = 10.64FDD159 pKa = 4.47IIEE162 pKa = 4.66SINIYY167 pKa = 10.81NFLEE171 pKa = 4.63AYY173 pKa = 9.72NLNHH177 pKa = 6.37SFYY180 pKa = 11.21DD181 pKa = 3.44LAEE184 pKa = 4.44RR185 pKa = 11.84EE186 pKa = 4.21FQEE189 pKa = 4.39LLGVAA194 pKa = 4.42

Molecular weight: 22.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5GZ22|A5GZ22_9CAUD Uncharacterized protein OS=Lactococcus phage phismq86 OX=444474 PE=4 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84GCFFVIIIYY11 pKa = 10.69SMNNIEE17 pKa = 4.97SVYY20 pKa = 10.77LNILRR25 pKa = 11.84GVKK28 pKa = 10.36NNLTNIIHH36 pKa = 6.54YY37 pKa = 8.36IQPNINIGDD46 pKa = 3.92VLSTTIALFALWYY59 pKa = 7.77TIHH62 pKa = 6.15STRR65 pKa = 11.84DD66 pKa = 3.43NQRR69 pKa = 11.84KK70 pKa = 8.93SVLPYY75 pKa = 7.97MTLEE79 pKa = 4.28QIRR82 pKa = 11.84STVTVDD88 pKa = 4.55GIGHH92 pKa = 6.04NTTVVDD98 pKa = 3.64TTSDD102 pKa = 3.48YY103 pKa = 11.11KK104 pKa = 11.47VRR106 pKa = 11.84VTKK109 pKa = 10.79NKK111 pKa = 8.09EE112 pKa = 3.81LKK114 pKa = 10.73GIEE117 pKa = 3.84VWGEE121 pKa = 3.85EE122 pKa = 3.99EE123 pKa = 4.09RR124 pKa = 11.84KK125 pKa = 10.48LIINEE130 pKa = 3.71KK131 pKa = 10.47SEE133 pKa = 4.35GKK135 pKa = 10.35GALSYY140 pKa = 11.43GSINFPRR147 pKa = 11.84SFKK150 pKa = 10.88LINTGKK156 pKa = 8.15NTAISFSLIVGKK168 pKa = 10.68KK169 pKa = 6.0STYY172 pKa = 9.57VKK174 pKa = 10.48NIAVNEE180 pKa = 4.04EE181 pKa = 4.24KK182 pKa = 10.33IISVLLEE189 pKa = 4.47DD190 pKa = 4.47EE191 pKa = 4.6NSTFNLLIKK200 pKa = 10.8FKK202 pKa = 10.52DD203 pKa = 3.28IYY205 pKa = 10.61GNQYY209 pKa = 9.39KK210 pKa = 10.32EE211 pKa = 4.03KK212 pKa = 9.84IEE214 pKa = 4.47FYY216 pKa = 9.67RR217 pKa = 11.84GRR219 pKa = 11.84MFLHH223 pKa = 7.12LDD225 pKa = 3.84LKK227 pKa = 10.76RR228 pKa = 11.84IRR230 pKa = 11.84FSKK233 pKa = 9.38TKK235 pKa = 10.36SIFMKK240 pKa = 9.45WKK242 pKa = 9.74SRR244 pKa = 11.84LKK246 pKa = 10.11IRR248 pKa = 11.84KK249 pKa = 6.66QARR252 pKa = 11.84NEE254 pKa = 3.8KK255 pKa = 10.55NKK257 pKa = 10.26LDD259 pKa = 3.72KK260 pKa = 11.23

Molecular weight: 30.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

10604

40

1027

207.9

23.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.469 ± 0.455

0.538 ± 0.153

6.375 ± 0.254

7.422 ± 0.793

4.14 ± 0.219

6.582 ± 0.463

1.358 ± 0.165

7.318 ± 0.499

8.223 ± 0.484

7.912 ± 0.419

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.48 ± 0.274

5.828 ± 0.281

2.942 ± 0.187

4.121 ± 0.221

3.838 ± 0.356

6.846 ± 0.339

6.884 ± 0.69

5.988 ± 0.242

1.245 ± 0.138

3.489 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski