Amino acid dipepetide frequency for Lactococcus phage Q54

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.95AlaAla: 7.95 ± 1.005
0.124AlaCys: 0.124 ± 0.15
3.354AlaAsp: 3.354 ± 0.559
4.472AlaGlu: 4.472 ± 0.693
2.733AlaPhe: 2.733 ± 0.595
4.224AlaGly: 4.224 ± 0.911
0.745AlaHis: 0.745 ± 0.294
3.975AlaIle: 3.975 ± 0.56
5.714AlaLys: 5.714 ± 1.093
6.832AlaLeu: 6.832 ± 1.064
2.484AlaMet: 2.484 ± 0.662
4.472AlaAsn: 4.472 ± 0.779
1.118AlaPro: 1.118 ± 0.332
2.484AlaGln: 2.484 ± 0.838
2.609AlaArg: 2.609 ± 0.537
3.354AlaSer: 3.354 ± 0.766
6.584AlaThr: 6.584 ± 0.962
6.335AlaVal: 6.335 ± 0.692
0.621AlaTrp: 0.621 ± 0.319
4.224AlaTyr: 4.224 ± 0.722
0.0AlaXaa: 0.0 ± 0.0
Cys
0.124CysAla: 0.124 ± 0.124
0.0CysCys: 0.0 ± 0.0
0.124CysAsp: 0.124 ± 0.126
0.124CysGlu: 0.124 ± 0.118
0.124CysPhe: 0.124 ± 0.116
0.497CysGly: 0.497 ± 0.391
0.248CysHis: 0.248 ± 0.278
0.124CysIle: 0.124 ± 0.116
0.248CysLys: 0.248 ± 0.199
0.373CysLeu: 0.373 ± 0.216
0.124CysMet: 0.124 ± 0.118
0.0CysAsn: 0.0 ± 0.0
0.373CysPro: 0.373 ± 0.246
0.248CysGln: 0.248 ± 0.159
0.248CysArg: 0.248 ± 0.176
0.124CysSer: 0.124 ± 0.116
0.373CysThr: 0.373 ± 0.204
0.124CysVal: 0.124 ± 0.139
0.0CysTrp: 0.0 ± 0.0
0.124CysTyr: 0.124 ± 0.126
0.0CysXaa: 0.0 ± 0.0
Asp
3.975AspAla: 3.975 ± 0.592
0.124AspCys: 0.124 ± 0.139
3.23AspAsp: 3.23 ± 0.729
3.851AspGlu: 3.851 ± 0.725
3.23AspPhe: 3.23 ± 0.64
3.727AspGly: 3.727 ± 0.64
0.373AspHis: 0.373 ± 0.206
2.857AspIle: 2.857 ± 0.515
5.963AspLys: 5.963 ± 0.611
4.348AspLeu: 4.348 ± 0.75
0.87AspMet: 0.87 ± 0.34
2.733AspAsn: 2.733 ± 0.459
3.106AspPro: 3.106 ± 0.741
1.366AspGln: 1.366 ± 0.356
1.615AspArg: 1.615 ± 0.435
3.354AspSer: 3.354 ± 0.521
3.602AspThr: 3.602 ± 0.765
3.851AspVal: 3.851 ± 0.751
0.87AspTrp: 0.87 ± 0.523
3.106AspTyr: 3.106 ± 0.538
0.0AspXaa: 0.0 ± 0.0
Glu
6.584GluAla: 6.584 ± 0.771
0.0GluCys: 0.0 ± 0.0
3.602GluAsp: 3.602 ± 0.806
5.466GluGlu: 5.466 ± 1.231
5.217GluPhe: 5.217 ± 0.753
3.23GluGly: 3.23 ± 0.503
0.124GluHis: 0.124 ± 0.101
3.975GluIle: 3.975 ± 0.648
7.453GluLys: 7.453 ± 1.024
9.068GluLeu: 9.068 ± 0.984
1.739GluMet: 1.739 ± 0.333
3.354GluAsn: 3.354 ± 0.612
1.739GluPro: 1.739 ± 0.514
4.224GluGln: 4.224 ± 0.723
3.354GluArg: 3.354 ± 0.865
3.23GluSer: 3.23 ± 0.731
3.23GluThr: 3.23 ± 0.778
5.217GluVal: 5.217 ± 0.963
1.118GluTrp: 1.118 ± 0.308
2.733GluTyr: 2.733 ± 0.644
0.0GluXaa: 0.0 ± 0.0
Phe
2.112PheAla: 2.112 ± 0.473
0.373PheCys: 0.373 ± 0.212
2.857PheAsp: 2.857 ± 0.579
3.975PheGlu: 3.975 ± 0.778
1.863PhePhe: 1.863 ± 0.626
3.975PheGly: 3.975 ± 0.789
0.248PheHis: 0.248 ± 0.164
2.981PheIle: 2.981 ± 0.649
3.602PheLys: 3.602 ± 0.611
2.484PheLeu: 2.484 ± 0.471
1.242PheMet: 1.242 ± 0.438
3.602PheAsn: 3.602 ± 0.641
0.87PhePro: 0.87 ± 0.253
0.745PheGln: 0.745 ± 0.279
1.366PheArg: 1.366 ± 0.429
2.857PheSer: 2.857 ± 0.736
3.354PheThr: 3.354 ± 0.558
2.484PheVal: 2.484 ± 0.613
0.373PheTrp: 0.373 ± 0.202
2.484PheTyr: 2.484 ± 0.494
0.0PheXaa: 0.0 ± 0.0
Gly
6.087GlyAla: 6.087 ± 1.132
0.248GlyCys: 0.248 ± 0.186
3.602GlyAsp: 3.602 ± 0.615
4.472GlyGlu: 4.472 ± 0.801
2.609GlyPhe: 2.609 ± 0.479
4.72GlyGly: 4.72 ± 0.809
0.87GlyHis: 0.87 ± 0.41
4.472GlyIle: 4.472 ± 0.643
5.342GlyLys: 5.342 ± 1.233
4.969GlyLeu: 4.969 ± 0.935
2.236GlyMet: 2.236 ± 0.58
3.478GlyAsn: 3.478 ± 0.708
0.124GlyPro: 0.124 ± 0.108
1.988GlyGln: 1.988 ± 0.558
3.23GlyArg: 3.23 ± 0.688
4.596GlySer: 4.596 ± 0.976
4.596GlyThr: 4.596 ± 0.901
3.354GlyVal: 3.354 ± 0.852
1.242GlyTrp: 1.242 ± 0.475
3.106GlyTyr: 3.106 ± 0.499
0.0GlyXaa: 0.0 ± 0.0
His
1.118HisAla: 1.118 ± 0.318
0.0HisCys: 0.0 ± 0.0
0.994HisAsp: 0.994 ± 0.299
1.118HisGlu: 1.118 ± 0.349
0.994HisPhe: 0.994 ± 0.413
1.242HisGly: 1.242 ± 0.451
0.497HisHis: 0.497 ± 0.26
0.621HisIle: 0.621 ± 0.251
0.621HisLys: 0.621 ± 0.328
0.87HisLeu: 0.87 ± 0.379
0.124HisMet: 0.124 ± 0.139
0.745HisAsn: 0.745 ± 0.341
0.248HisPro: 0.248 ± 0.174
0.0HisGln: 0.0 ± 0.0
0.621HisArg: 0.621 ± 0.264
0.497HisSer: 0.497 ± 0.305
0.745HisThr: 0.745 ± 0.327
0.497HisVal: 0.497 ± 0.334
0.0HisTrp: 0.0 ± 0.0
0.994HisTyr: 0.994 ± 0.296
0.0HisXaa: 0.0 ± 0.0
Ile
4.472IleAla: 4.472 ± 0.667
0.248IleCys: 0.248 ± 0.182
3.106IleAsp: 3.106 ± 0.584
4.72IleGlu: 4.72 ± 0.777
2.609IlePhe: 2.609 ± 0.53
3.975IleGly: 3.975 ± 0.581
0.497IleHis: 0.497 ± 0.231
1.863IleIle: 1.863 ± 0.526
4.596IleLys: 4.596 ± 0.757
4.348IleLeu: 4.348 ± 0.813
1.242IleMet: 1.242 ± 0.373
4.72IleAsn: 4.72 ± 0.82
1.615IlePro: 1.615 ± 0.426
2.733IleGln: 2.733 ± 0.48
2.236IleArg: 2.236 ± 0.414
3.354IleSer: 3.354 ± 0.515
3.727IleThr: 3.727 ± 0.811
4.224IleVal: 4.224 ± 0.664
0.373IleTrp: 0.373 ± 0.217
1.988IleTyr: 1.988 ± 0.477
0.0IleXaa: 0.0 ± 0.0
Lys
7.702LysAla: 7.702 ± 1.106
0.248LysCys: 0.248 ± 0.183
4.72LysAsp: 4.72 ± 0.759
6.087LysGlu: 6.087 ± 1.178
1.739LysPhe: 1.739 ± 0.39
5.714LysGly: 5.714 ± 1.003
1.491LysHis: 1.491 ± 0.449
5.217LysIle: 5.217 ± 0.716
9.689LysLys: 9.689 ± 1.548
7.578LysLeu: 7.578 ± 0.892
3.727LysMet: 3.727 ± 0.645
4.72LysAsn: 4.72 ± 0.8
2.112LysPro: 2.112 ± 0.475
3.602LysGln: 3.602 ± 0.602
4.472LysArg: 4.472 ± 0.833
3.106LysSer: 3.106 ± 0.64
6.211LysThr: 6.211 ± 0.667
6.087LysVal: 6.087 ± 0.672
0.87LysTrp: 0.87 ± 0.355
3.602LysTyr: 3.602 ± 0.654
0.0LysXaa: 0.0 ± 0.0
Leu
6.708LeuAla: 6.708 ± 1.105
0.124LeuCys: 0.124 ± 0.139
5.59LeuAsp: 5.59 ± 0.776
6.46LeuGlu: 6.46 ± 0.883
2.857LeuPhe: 2.857 ± 0.724
4.845LeuGly: 4.845 ± 0.855
1.863LeuHis: 1.863 ± 0.531
4.72LeuIle: 4.72 ± 0.705
7.826LeuLys: 7.826 ± 1.202
6.832LeuLeu: 6.832 ± 1.192
2.609LeuMet: 2.609 ± 0.618
5.217LeuAsn: 5.217 ± 0.864
2.609LeuPro: 2.609 ± 0.535
3.106LeuGln: 3.106 ± 0.58
3.478LeuArg: 3.478 ± 0.667
6.211LeuSer: 6.211 ± 0.814
7.578LeuThr: 7.578 ± 0.845
6.211LeuVal: 6.211 ± 0.756
0.621LeuTrp: 0.621 ± 0.325
2.857LeuTyr: 2.857 ± 0.697
0.0LeuXaa: 0.0 ± 0.0
Met
2.112MetAla: 2.112 ± 0.587
0.124MetCys: 0.124 ± 0.116
0.745MetAsp: 0.745 ± 0.302
2.484MetGlu: 2.484 ± 0.479
0.745MetPhe: 0.745 ± 0.37
1.118MetGly: 1.118 ± 0.366
0.0MetHis: 0.0 ± 0.0
0.994MetIle: 0.994 ± 0.424
3.851MetLys: 3.851 ± 0.656
2.981MetLeu: 2.981 ± 0.669
0.248MetMet: 0.248 ± 0.162
1.366MetAsn: 1.366 ± 0.334
0.745MetPro: 0.745 ± 0.356
0.621MetGln: 0.621 ± 0.293
0.745MetArg: 0.745 ± 0.363
2.36MetSer: 2.36 ± 0.49
2.484MetThr: 2.484 ± 0.569
1.615MetVal: 1.615 ± 0.394
0.124MetTrp: 0.124 ± 0.137
0.87MetTyr: 0.87 ± 0.396
0.0MetXaa: 0.0 ± 0.0
Asn
3.851AsnAla: 3.851 ± 1.04
0.373AsnCys: 0.373 ± 0.212
3.106AsnAsp: 3.106 ± 0.628
4.348AsnGlu: 4.348 ± 0.74
2.112AsnPhe: 2.112 ± 0.484
4.099AsnGly: 4.099 ± 0.666
0.745AsnHis: 0.745 ± 0.343
3.478AsnIle: 3.478 ± 1.003
5.217AsnLys: 5.217 ± 0.744
5.342AsnLeu: 5.342 ± 0.986
0.994AsnMet: 0.994 ± 0.329
4.348AsnAsn: 4.348 ± 0.796
2.36AsnPro: 2.36 ± 0.468
1.739AsnGln: 1.739 ± 0.679
2.857AsnArg: 2.857 ± 0.583
3.478AsnSer: 3.478 ± 0.57
3.602AsnThr: 3.602 ± 0.645
4.224AsnVal: 4.224 ± 0.668
0.497AsnTrp: 0.497 ± 0.264
3.23AsnTyr: 3.23 ± 0.804
0.0AsnXaa: 0.0 ± 0.0
Pro
1.242ProAla: 1.242 ± 0.319
0.0ProCys: 0.0 ± 0.0
1.739ProAsp: 1.739 ± 0.463
1.988ProGlu: 1.988 ± 0.453
1.366ProPhe: 1.366 ± 0.551
1.491ProGly: 1.491 ± 0.346
0.248ProHis: 0.248 ± 0.215
1.615ProIle: 1.615 ± 0.384
2.609ProLys: 2.609 ± 0.58
2.857ProLeu: 2.857 ± 0.629
0.87ProMet: 0.87 ± 0.285
2.981ProAsn: 2.981 ± 0.781
1.366ProPro: 1.366 ± 0.442
1.242ProGln: 1.242 ± 0.314
0.621ProArg: 0.621 ± 0.252
1.739ProSer: 1.739 ± 0.476
1.615ProThr: 1.615 ± 0.4
1.615ProVal: 1.615 ± 0.564
0.248ProTrp: 0.248 ± 0.247
0.87ProTyr: 0.87 ± 0.377
0.0ProXaa: 0.0 ± 0.0
Gln
2.36GlnAla: 2.36 ± 0.535
0.0GlnCys: 0.0 ± 0.0
1.366GlnAsp: 1.366 ± 0.359
2.857GlnGlu: 2.857 ± 0.619
1.615GlnPhe: 1.615 ± 0.382
2.236GlnGly: 2.236 ± 0.793
0.621GlnHis: 0.621 ± 0.305
2.112GlnIle: 2.112 ± 0.514
2.36GlnLys: 2.36 ± 0.574
3.478GlnLeu: 3.478 ± 0.655
1.242GlnMet: 1.242 ± 0.373
1.988GlnAsn: 1.988 ± 0.475
0.621GlnPro: 0.621 ± 0.201
2.112GlnGln: 2.112 ± 1.121
1.863GlnArg: 1.863 ± 0.657
1.988GlnSer: 1.988 ± 0.378
2.36GlnThr: 2.36 ± 0.589
1.739GlnVal: 1.739 ± 0.401
0.621GlnTrp: 0.621 ± 0.305
1.491GlnTyr: 1.491 ± 0.388
0.0GlnXaa: 0.0 ± 0.0
Arg
2.609ArgAla: 2.609 ± 0.653
0.124ArgCys: 0.124 ± 0.118
1.863ArgAsp: 1.863 ± 0.489
3.851ArgGlu: 3.851 ± 0.658
1.491ArgPhe: 1.491 ± 0.461
3.602ArgGly: 3.602 ± 0.689
1.118ArgHis: 1.118 ± 0.363
2.112ArgIle: 2.112 ± 0.579
3.354ArgLys: 3.354 ± 0.886
3.602ArgLeu: 3.602 ± 0.668
0.994ArgMet: 0.994 ± 0.285
2.733ArgAsn: 2.733 ± 0.72
0.497ArgPro: 0.497 ± 0.235
1.491ArgGln: 1.491 ± 0.386
1.491ArgArg: 1.491 ± 0.421
2.484ArgSer: 2.484 ± 0.519
1.739ArgThr: 1.739 ± 0.525
2.112ArgVal: 2.112 ± 0.436
0.373ArgTrp: 0.373 ± 0.254
1.366ArgTyr: 1.366 ± 0.434
0.0ArgXaa: 0.0 ± 0.0
Ser
3.23SerAla: 3.23 ± 0.853
0.621SerCys: 0.621 ± 0.269
3.478SerAsp: 3.478 ± 0.679
5.342SerGlu: 5.342 ± 0.914
3.478SerPhe: 3.478 ± 0.543
3.727SerGly: 3.727 ± 0.811
0.621SerHis: 0.621 ± 0.373
3.23SerIle: 3.23 ± 0.677
4.596SerLys: 4.596 ± 0.758
4.72SerLeu: 4.72 ± 0.848
0.87SerMet: 0.87 ± 0.36
4.099SerAsn: 4.099 ± 0.835
2.112SerPro: 2.112 ± 0.61
1.863SerGln: 1.863 ± 0.457
1.242SerArg: 1.242 ± 0.327
2.484SerSer: 2.484 ± 0.568
3.23SerThr: 3.23 ± 0.621
3.354SerVal: 3.354 ± 0.527
0.87SerTrp: 0.87 ± 0.403
2.981SerTyr: 2.981 ± 0.63
0.0SerXaa: 0.0 ± 0.0
Thr
5.59ThrAla: 5.59 ± 0.951
0.248ThrCys: 0.248 ± 0.171
4.224ThrAsp: 4.224 ± 0.79
5.217ThrGlu: 5.217 ± 0.96
4.099ThrPhe: 4.099 ± 0.681
5.217ThrGly: 5.217 ± 0.871
0.621ThrHis: 0.621 ± 0.233
5.466ThrIle: 5.466 ± 0.776
4.845ThrLys: 4.845 ± 0.539
6.46ThrLeu: 6.46 ± 0.758
1.118ThrMet: 1.118 ± 0.4
2.981ThrAsn: 2.981 ± 0.581
3.727ThrPro: 3.727 ± 0.847
2.36ThrGln: 2.36 ± 0.456
2.36ThrArg: 2.36 ± 0.504
3.478ThrSer: 3.478 ± 0.6
5.217ThrThr: 5.217 ± 1.2
4.224ThrVal: 4.224 ± 1.01
0.373ThrTrp: 0.373 ± 0.286
3.23ThrTyr: 3.23 ± 0.822
0.0ThrXaa: 0.0 ± 0.0
Val
3.851ValAla: 3.851 ± 0.711
0.497ValCys: 0.497 ± 0.281
4.596ValAsp: 4.596 ± 0.807
4.224ValGlu: 4.224 ± 0.597
2.609ValPhe: 2.609 ± 0.542
4.348ValGly: 4.348 ± 0.689
0.745ValHis: 0.745 ± 0.296
4.348ValIle: 4.348 ± 0.754
4.969ValLys: 4.969 ± 0.85
4.969ValLeu: 4.969 ± 0.869
1.491ValMet: 1.491 ± 0.563
4.099ValAsn: 4.099 ± 0.713
1.739ValPro: 1.739 ± 0.514
1.366ValGln: 1.366 ± 0.428
1.988ValArg: 1.988 ± 0.5
4.224ValSer: 4.224 ± 0.685
6.957ValThr: 6.957 ± 1.03
3.478ValVal: 3.478 ± 0.657
0.497ValTrp: 0.497 ± 0.311
3.23ValTyr: 3.23 ± 0.708
0.0ValXaa: 0.0 ± 0.0
Trp
0.87TrpAla: 0.87 ± 0.226
0.0TrpCys: 0.0 ± 0.0
0.745TrpAsp: 0.745 ± 0.34
0.87TrpGlu: 0.87 ± 0.367
0.497TrpPhe: 0.497 ± 0.322
0.745TrpGly: 0.745 ± 0.29
0.373TrpHis: 0.373 ± 0.211
0.124TrpIle: 0.124 ± 0.125
0.994TrpLys: 0.994 ± 0.305
1.366TrpLeu: 1.366 ± 0.469
0.373TrpMet: 0.373 ± 0.195
0.248TrpAsn: 0.248 ± 0.205
0.0TrpPro: 0.0 ± 0.0
0.373TrpGln: 0.373 ± 0.228
0.621TrpArg: 0.621 ± 0.334
0.124TrpSer: 0.124 ± 0.101
0.745TrpThr: 0.745 ± 0.351
0.373TrpVal: 0.373 ± 0.19
0.124TrpTrp: 0.124 ± 0.142
0.87TrpTyr: 0.87 ± 0.324
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.863TyrAla: 1.863 ± 0.549
0.248TyrCys: 0.248 ± 0.179
3.106TyrAsp: 3.106 ± 0.609
2.733TyrGlu: 2.733 ± 0.596
1.863TyrPhe: 1.863 ± 0.437
2.609TyrGly: 2.609 ± 0.562
0.497TyrHis: 0.497 ± 0.231
2.36TyrIle: 2.36 ± 0.423
4.845TyrLys: 4.845 ± 0.916
4.845TyrLeu: 4.845 ± 0.863
1.615TyrMet: 1.615 ± 0.469
2.112TyrAsn: 2.112 ± 0.479
1.242TyrPro: 1.242 ± 0.322
1.366TyrGln: 1.366 ± 0.445
1.988TyrArg: 1.988 ± 0.599
3.106TyrSer: 3.106 ± 0.512
3.106TyrThr: 3.106 ± 0.481
3.23TyrVal: 3.23 ± 0.624
0.621TyrTrp: 0.621 ± 0.244
1.739TyrTyr: 1.739 ± 0.434
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (8051 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski