Thermoproteus tenax virus 1 (strain KRA1) (TTV1)
Average proteome isoelectric point is 7.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P19307|YORW_TTV1K Uncharacterized 12.1 kDa protein OS=Thermoproteus tenax virus 1 (strain KRA1) OX=10480 PE=4 SV=1
MM1 pKa = 7.73 FEE3 pKa = 4.25 PCLYY7 pKa = 9.82 TFTFAVLPFIDD18 pKa = 4.77 KK19 pKa = 10.82 YY20 pKa = 9.41 ITNGSVNAANCDD32 pKa = 3.05 ISISFTTEE40 pKa = 3.45 YY41 pKa = 10.92 VPDD44 pKa = 4.3 IFCSMLTFF52 pKa = 4.83
Molecular weight: 5.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.946
IPC2_protein 3.834
IPC_protein 3.528
Toseland 3.401
ProMoST 3.808
Dawson 3.579
Bjellqvist 3.77
Wikipedia 3.605
Rodwell 3.427
Grimsley 3.338
Solomon 3.478
Lehninger 3.427
Nozaki 3.783
DTASelect 3.872
Thurlkill 3.528
EMBOSS 3.605
Sillero 3.681
Patrickios 0.006
IPC_peptide 3.465
IPC2_peptide 3.63
IPC2.peptide.svr19 3.716
Protein with the highest isoelectric point:
>sp|P19295|YORK_TTV1K Uncharacterized 10.5 kDa protein OS=Thermoproteus tenax virus 1 (strain KRA1) OX=10480 PE=4 SV=1
MM1 pKa = 7.93 DD2 pKa = 3.35 MHH4 pKa = 6.96 MLRR7 pKa = 11.84 LLDD10 pKa = 3.8 LQCILHH16 pKa = 6.36 HH17 pKa = 6.57 RR18 pKa = 11.84 LQEE21 pKa = 3.99 RR22 pKa = 11.84 MRR24 pKa = 11.84 ATSVKK29 pKa = 9.8 LRR31 pKa = 11.84 QYY33 pKa = 10.31 PQILQQHH40 pKa = 6.08 RR41 pKa = 11.84 CGLHH45 pKa = 5.66 PRR47 pKa = 11.84 SLLL50 pKa = 3.72
Molecular weight: 6.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.589
IPC_protein 10.789
Toseland 10.774
ProMoST 10.877
Dawson 10.847
Bjellqvist 10.73
Wikipedia 11.184
Rodwell 10.745
Grimsley 10.906
Solomon 11.14
Lehninger 11.082
Nozaki 10.818
DTASelect 10.716
Thurlkill 10.789
EMBOSS 11.228
Sillero 10.818
Patrickios 10.657
IPC_peptide 11.14
IPC2_peptide 10.248
IPC2.peptide.svr19 8.749
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
37
0
37
4377
50
360
118.3
13.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.186 ± 0.449
1.668 ± 0.276
4.432 ± 0.403
5.003 ± 0.694
4.409 ± 0.393
6.077 ± 0.551
1.645 ± 0.277
9.801 ± 0.562
4.935 ± 0.657
8.91 ± 0.636
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.153 ± 0.3
4.775 ± 0.458
4.455 ± 0.613
2.879 ± 0.549
4.958 ± 0.601
7.608 ± 0.686
6.191 ± 0.763
6.214 ± 0.441
1.074 ± 0.166
6.626 ± 0.474
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here