Bovine faeces associated smacovirus 4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Cosmacovirus; Cosmacovirus bovas1

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160HX24|A0A160HX24_9VIRU Replication associated protein OS=Bovine faeces associated smacovirus 4 OX=1843752 PE=4 SV=1
MM1 pKa = 6.36VTVTVSEE8 pKa = 4.52TYY10 pKa = 10.75DD11 pKa = 3.64LSTKK15 pKa = 8.29VNKK18 pKa = 9.5MSLIGIHH25 pKa = 5.95TPGKK29 pKa = 10.29SLIQKK34 pKa = 9.0SYY36 pKa = 10.79PGLLMNSKK44 pKa = 10.25YY45 pKa = 10.72ISIDD49 pKa = 3.36KK50 pKa = 10.88VDD52 pKa = 3.5VTLAAVSTLPISPDD66 pKa = 3.41QVGLDD71 pKa = 3.66VDD73 pKa = 4.88NIAPQDD79 pKa = 3.59MMNPILYY86 pKa = 9.82KK87 pKa = 10.58AVSNDD92 pKa = 2.82SMSNIEE98 pKa = 4.29ARR100 pKa = 11.84LHH102 pKa = 5.49GLGFGSGTQVSGSMVDD118 pKa = 4.19AINDD122 pKa = 3.59NVTGISDD129 pKa = 3.95EE130 pKa = 4.36FNVYY134 pKa = 10.57YY135 pKa = 10.74SLLSNRR141 pKa = 11.84DD142 pKa = 3.17GFRR145 pKa = 11.84IAHH148 pKa = 5.69PQQGLSMRR156 pKa = 11.84GLVPLVFDD164 pKa = 4.16KK165 pKa = 11.06YY166 pKa = 9.17YY167 pKa = 11.09NVGAFMTNSQEE178 pKa = 4.11VTDD181 pKa = 4.24VGDD184 pKa = 3.59TGADD188 pKa = 3.48EE189 pKa = 4.52TPSGDD194 pKa = 4.48LYY196 pKa = 10.31PTLQYY201 pKa = 11.08SAGSLSVVNRR211 pKa = 11.84PVQAMRR217 pKa = 11.84GRR219 pKa = 11.84PHH221 pKa = 7.69RR222 pKa = 11.84MPRR225 pKa = 11.84FPTLYY230 pKa = 8.86VTAASSGSSQSTNQRR245 pKa = 11.84VANGIADD252 pKa = 4.21GQPLNCEE259 pKa = 3.92VLMPDD264 pKa = 4.01ILPVYY269 pKa = 9.86LGMIIMPPAKK279 pKa = 8.57RR280 pKa = 11.84TVMFYY285 pKa = 11.05RR286 pKa = 11.84MVVRR290 pKa = 11.84ATLTFSEE297 pKa = 4.57VRR299 pKa = 11.84PMSEE303 pKa = 3.39ITSFAGLNSGYY314 pKa = 10.5GSEE317 pKa = 4.98VYY319 pKa = 8.8HH320 pKa = 6.71TDD322 pKa = 2.93YY323 pKa = 11.66ALQSKK328 pKa = 9.01QMEE331 pKa = 4.67STTDD335 pKa = 3.58LVDD338 pKa = 3.45VQNANIEE345 pKa = 4.31KK346 pKa = 10.25IMEE349 pKa = 4.16GRR351 pKa = 3.45

Molecular weight: 38.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160HX24|A0A160HX24_9VIRU Replication associated protein OS=Bovine faeces associated smacovirus 4 OX=1843752 PE=4 SV=1
MM1 pKa = 7.88TIPQSEE7 pKa = 4.21MSKK10 pKa = 10.73RR11 pKa = 11.84EE12 pKa = 3.74LLHH15 pKa = 5.25YY16 pKa = 10.16LRR18 pKa = 11.84HH19 pKa = 6.31RR20 pKa = 11.84SKK22 pKa = 9.59RR23 pKa = 11.84WQFGRR28 pKa = 11.84EE29 pKa = 3.67TGRR32 pKa = 11.84NTGYY36 pKa = 9.93RR37 pKa = 11.84HH38 pKa = 4.48YY39 pKa = 10.24QVRR42 pKa = 11.84YY43 pKa = 8.78EE44 pKa = 4.41SSNDD48 pKa = 3.52DD49 pKa = 3.0IGRR52 pKa = 11.84EE53 pKa = 3.46RR54 pKa = 11.84LYY56 pKa = 10.3WVGIACEE63 pKa = 4.46LEE65 pKa = 4.33PSNGWSNYY73 pKa = 6.52EE74 pKa = 3.99LKK76 pKa = 11.05DD77 pKa = 3.36GDD79 pKa = 5.1FYY81 pKa = 11.27TSDD84 pKa = 3.68DD85 pKa = 4.0ADD87 pKa = 3.67LGKK90 pKa = 10.72YY91 pKa = 9.54RR92 pKa = 11.84FGKK95 pKa = 9.59LRR97 pKa = 11.84KK98 pKa = 8.51FQTRR102 pKa = 11.84LLEE105 pKa = 4.1YY106 pKa = 10.72LEE108 pKa = 4.43SRR110 pKa = 11.84NDD112 pKa = 3.29RR113 pKa = 11.84TVGIVVDD120 pKa = 3.78HH121 pKa = 6.5VGGKK125 pKa = 9.92GKK127 pKa = 8.97TYY129 pKa = 9.82FARR132 pKa = 11.84YY133 pKa = 9.13CSLNHH138 pKa = 6.4KK139 pKa = 9.91AVYY142 pKa = 10.03LHH144 pKa = 7.31GSGTDD149 pKa = 3.36NQVARR154 pKa = 11.84DD155 pKa = 3.98LYY157 pKa = 10.96DD158 pKa = 3.22ISEE161 pKa = 4.33SEE163 pKa = 4.69RR164 pKa = 11.84INAVIIDD171 pKa = 4.01CTRR174 pKa = 11.84SEE176 pKa = 4.33NNLSEE181 pKa = 4.2SDD183 pKa = 3.47FWNGIEE189 pKa = 4.38QIKK192 pKa = 10.6NGHH195 pKa = 5.94LCDD198 pKa = 3.1RR199 pKa = 11.84RR200 pKa = 11.84YY201 pKa = 10.64GYY203 pKa = 9.8RR204 pKa = 11.84EE205 pKa = 3.32KK206 pKa = 10.26WIRR209 pKa = 11.84PPAVLILSNHH219 pKa = 6.09DD220 pKa = 4.3PDD222 pKa = 4.04WKK224 pKa = 10.7CLSEE228 pKa = 4.58DD229 pKa = 3.02RR230 pKa = 11.84WHH232 pKa = 6.99KK233 pKa = 10.48FFIKK237 pKa = 9.67EE238 pKa = 3.71DD239 pKa = 3.32AMYY242 pKa = 10.32EE243 pKa = 4.03YY244 pKa = 11.03YY245 pKa = 10.43KK246 pKa = 10.45RR247 pKa = 11.84EE248 pKa = 4.02KK249 pKa = 10.64KK250 pKa = 8.95VTSLL254 pKa = 3.64

Molecular weight: 30.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

605

254

351

302.5

34.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.628 ± 0.935

0.992 ± 0.618

6.777 ± 0.445

5.289 ± 1.635

2.81 ± 0.215

7.438 ± 0.222

2.314 ± 0.778

5.289 ± 0.108

4.628 ± 1.306

7.934 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.636 ± 1.553

5.289 ± 0.108

4.132 ± 1.369

3.471 ± 0.702

6.612 ± 2.044

9.091 ± 1.019

5.62 ± 1.065

7.273 ± 1.861

1.157 ± 1.012

5.62 ± 0.928
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski