Erwinia phage vB_EamM_Yoloswag

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Yoloswagvirus; Erwinia virus Yoloswag

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 333 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S6L3G4|A0A1S6L3G4_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_Yoloswag OX=1958956 GN=YOLOSWAG_242 PE=4 SV=1
MM1 pKa = 7.26IASTINATYY10 pKa = 7.48EE11 pKa = 4.29TTNMTTNIFPEE22 pKa = 4.23LNIDD26 pKa = 3.66EE27 pKa = 4.44LTAFVNRR34 pKa = 11.84INPQLNKK41 pKa = 10.13NVKK44 pKa = 10.02LCVEE48 pKa = 4.18SHH50 pKa = 7.41DD51 pKa = 5.55DD52 pKa = 3.75EE53 pKa = 7.28DD54 pKa = 4.35NTGHH58 pKa = 6.95IYY60 pKa = 10.59AVEE63 pKa = 3.84VDD65 pKa = 4.14EE66 pKa = 5.64NGEE69 pKa = 4.35DD70 pKa = 4.39SDD72 pKa = 6.09AMWGWLIDD80 pKa = 3.95VPCAEE85 pKa = 4.34CEE87 pKa = 3.96KK88 pKa = 10.61FYY90 pKa = 11.08FMYY93 pKa = 10.56GGNFPHH99 pKa = 6.85DD100 pKa = 3.65QEE102 pKa = 5.09GSCEE106 pKa = 3.87IDD108 pKa = 3.37DD109 pKa = 4.34ASFIAKK115 pKa = 9.8VNSLEE120 pKa = 4.04FF121 pKa = 4.72

Molecular weight: 13.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S6L3Q9|A0A1S6L3Q9_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_Yoloswag OX=1958956 GN=YOLOSWAG_333 PE=4 SV=1
MM1 pKa = 7.71PGRR4 pKa = 11.84RR5 pKa = 11.84LHH7 pKa = 6.06PRR9 pKa = 11.84DD10 pKa = 2.98WGLAPFLKK18 pKa = 10.65LILISQFSKK27 pKa = 11.08GFDD30 pKa = 3.09TLEE33 pKa = 4.53RR34 pKa = 11.84IWIYY38 pKa = 11.42AVFTNCALILNRR50 pKa = 11.84KK51 pKa = 8.29GNNNAYY57 pKa = 8.08ITQTSSSRR65 pKa = 3.36

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

333

0

333

82302

39

1951

247.2

27.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.545 ± 0.184

1.142 ± 0.056

6.849 ± 0.158

5.965 ± 0.189

3.798 ± 0.08

6.278 ± 0.157

1.962 ± 0.077

5.483 ± 0.096

5.527 ± 0.224

8.119 ± 0.136

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.639 ± 0.086

4.668 ± 0.127

4.061 ± 0.092

4.37 ± 0.093

5.689 ± 0.138

6.709 ± 0.125

6.377 ± 0.197

7.322 ± 0.144

1.084 ± 0.038

3.412 ± 0.084
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski