Clostridium phage phiCTP1
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D9ZND9|D9ZND9_9CAUD Hypothetical phage protein OS=Clostridium phage phiCTP1 OX=871584 GN=phiCTP1_gp15 PE=4 SV=1
MM1 pKa = 7.76 NDD3 pKa = 3.24 LEE5 pKa = 4.48 LLKK8 pKa = 10.55 FLIMEE13 pKa = 4.35 SKK15 pKa = 10.81 YY16 pKa = 10.69 PYY18 pKa = 10.48 FSDD21 pKa = 6.02 DD22 pKa = 4.36 DD23 pKa = 3.93 LQKK26 pKa = 11.31 YY27 pKa = 10.69 LDD29 pKa = 3.78 VNKK32 pKa = 10.75 GNVYY36 pKa = 8.89 LTASQLCLMKK46 pKa = 9.8 MDD48 pKa = 4.08 NEE50 pKa = 4.1 KK51 pKa = 10.88 SITVGPITIQNPDD64 pKa = 2.67 ASYY67 pKa = 9.0 WQNLSLQYY75 pKa = 10.9 ADD77 pKa = 5.21 KK78 pKa = 10.99 AATDD82 pKa = 3.74 DD83 pKa = 3.92 TSNGGTGGYY92 pKa = 8.45 YY93 pKa = 9.98 RR94 pKa = 11.84 NYY96 pKa = 9.15 MDD98 pKa = 5.39 RR99 pKa = 11.84 ADD101 pKa = 3.85 GQQ103 pKa = 3.6
Molecular weight: 11.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.923
IPC2_protein 4.228
IPC_protein 4.164
Toseland 3.935
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.139
Rodwell 3.986
Grimsley 3.846
Solomon 4.164
Lehninger 4.113
Nozaki 4.291
DTASelect 4.584
Thurlkill 4.012
EMBOSS 4.139
Sillero 4.291
Patrickios 2.003
IPC_peptide 4.151
IPC2_peptide 4.266
IPC2.peptide.svr19 4.181
Protein with the highest isoelectric point:
>tr|D9ZNL0|D9ZNL0_9CAUD Hypothetical phage protein OS=Clostridium phage phiCTP1 OX=871584 GN=phiCTP1_gp86 PE=4 SV=1
MM1 pKa = 7.81 IYY3 pKa = 10.04 LGKK6 pKa = 10.74 GGVFVATKK14 pKa = 9.9 IYY16 pKa = 10.33 DD17 pKa = 3.51 KK18 pKa = 11.13 QGFSILRR25 pKa = 11.84 AGDD28 pKa = 3.42 GFIVYY33 pKa = 10.21 NNSKK37 pKa = 10.37 AFKK40 pKa = 9.24 EE41 pKa = 4.5 GHH43 pKa = 4.97 SHH45 pKa = 6.44 LRR47 pKa = 11.84 NYY49 pKa = 8.48 SACIRR54 pKa = 11.84 VIYY57 pKa = 9.44 CIRR60 pKa = 11.84 HH61 pKa = 4.58 KK62 pKa = 10.49 KK63 pKa = 8.46 IPKK66 pKa = 7.88 RR67 pKa = 11.84 TSFYY71 pKa = 10.64 FLQSLIRR78 pKa = 11.84 VASDD82 pKa = 3.17 DD83 pKa = 3.91 DD84 pKa = 4.57 YY85 pKa = 11.62 KK86 pKa = 11.76 VKK88 pKa = 10.6 VQALIDD94 pKa = 3.59 VRR96 pKa = 11.84 VQKK99 pKa = 10.68 GKK101 pKa = 10.7 KK102 pKa = 8.74 RR103 pKa = 11.84 GYY105 pKa = 8.94 YY106 pKa = 9.97 NNRR109 pKa = 11.84 FF110 pKa = 3.45
Molecular weight: 12.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.239
IPC2_protein 9.721
IPC_protein 9.853
Toseland 10.204
ProMoST 9.897
Dawson 10.409
Bjellqvist 10.087
Wikipedia 10.584
Rodwell 10.877
Grimsley 10.482
Solomon 10.423
Lehninger 10.394
Nozaki 10.204
DTASelect 10.072
Thurlkill 10.248
EMBOSS 10.599
Sillero 10.321
Patrickios 10.526
IPC_peptide 10.423
IPC2_peptide 8.843
IPC2.peptide.svr19 8.629
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
86
0
86
18423
28
1029
214.2
24.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.481 ± 0.497
1.482 ± 0.145
6.894 ± 0.305
5.395 ± 0.306
3.941 ± 0.182
6.253 ± 0.363
1.46 ± 0.112
7.702 ± 0.263
9.097 ± 0.395
8.701 ± 0.237
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.692 ± 0.11
6.839 ± 0.167
2.785 ± 0.169
3.105 ± 0.121
3.279 ± 0.213
5.309 ± 0.324
6.541 ± 0.34
6.459 ± 0.187
0.955 ± 0.095
4.63 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here