Arthrobacter phage Adat
Average proteome isoelectric point is 5.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A249XLG9|A0A249XLG9_9CAUD Uncharacterized protein OS=Arthrobacter phage Adat OX=2027883 GN=ADAT_4 PE=4 SV=1
MM1 pKa = 7.67 EE2 pKa = 6.12 DD3 pKa = 3.41 EE4 pKa = 4.47 NEE6 pKa = 4.04 RR7 pKa = 11.84 TPTMKK12 pKa = 10.74 LLIEE16 pKa = 4.35 GSKK19 pKa = 10.75 VEE21 pKa = 4.21 ITPDD25 pKa = 3.39 TNASMYY31 pKa = 10.45 EE32 pKa = 3.88 YY33 pKa = 10.75 LQDD36 pKa = 3.49 NGLYY40 pKa = 10.34 QLMEE44 pKa = 4.6 EE45 pKa = 5.01 GKK47 pKa = 8.55 ATYY50 pKa = 8.92 PIHH53 pKa = 6.84 LPNGYY58 pKa = 9.05 FVEE61 pKa = 4.67 GVEE64 pKa = 4.62 ISS66 pKa = 3.18
Molecular weight: 7.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.941
IPC2_protein 4.253
IPC_protein 4.075
Toseland 3.935
ProMoST 4.19
Dawson 4.012
Bjellqvist 4.177
Wikipedia 3.872
Rodwell 3.923
Grimsley 3.859
Solomon 3.999
Lehninger 3.948
Nozaki 4.139
DTASelect 4.202
Thurlkill 3.961
EMBOSS 3.897
Sillero 4.19
Patrickios 1.914
IPC_peptide 4.012
IPC2_peptide 4.177
IPC2.peptide.svr19 4.074
Protein with the highest isoelectric point:
>tr|A0A249XLQ3|A0A249XLQ3_9CAUD Uncharacterized protein OS=Arthrobacter phage Adat OX=2027883 GN=ADAT_54 PE=4 SV=1
MM1 pKa = 6.44 EE2 pKa = 4.55 QEE4 pKa = 4.22 MFGAPIVRR12 pKa = 11.84 WDD14 pKa = 3.68 SLNPSAYY21 pKa = 9.94 GRR23 pKa = 11.84 VIVAGRR29 pKa = 11.84 RR30 pKa = 11.84 GGKK33 pKa = 6.39 TQRR36 pKa = 11.84 QRR38 pKa = 11.84 EE39 pKa = 4.09 WSSLLFDD46 pKa = 6.16 EE47 pKa = 5.65 ILEE50 pKa = 4.48 TQNLMNKK57 pKa = 9.96 AKK59 pKa = 10.11 VRR61 pKa = 11.84 DD62 pKa = 3.98 YY63 pKa = 11.28 IRR65 pKa = 11.84 TQSPLAEE72 pKa = 4.07 SVRR75 pKa = 11.84 PNYY78 pKa = 9.5 IRR80 pKa = 11.84 SRR82 pKa = 11.84 WVRR85 pKa = 11.84 FANGHH90 pKa = 4.77 GRR92 pKa = 11.84 NNAVVVFSLSLDD104 pKa = 3.68 VQGDD108 pKa = 3.55 QQLIFTMDD116 pKa = 3.34 QINRR120 pKa = 11.84 LSTTRR125 pKa = 11.84 EE126 pKa = 3.62 TIYY129 pKa = 10.69 RR130 pKa = 11.84 LSALSVTIAGTPLMTSFILSLDD152 pKa = 3.2 QLMEE156 pKa = 3.75 KK157 pKa = 10.03 RR158 pKa = 11.84 GYY160 pKa = 8.05 QFRR163 pKa = 11.84 QKK165 pKa = 10.67 VNPYY169 pKa = 9.68 PLWQEE174 pKa = 3.97 FF175 pKa = 3.37
Molecular weight: 20.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.262
IPC2_protein 9.487
IPC_protein 10.175
Toseland 10.058
ProMoST 9.897
Dawson 10.306
Bjellqvist 10.043
Wikipedia 10.54
Rodwell 10.438
Grimsley 10.409
Solomon 10.365
Lehninger 10.321
Nozaki 10.014
DTASelect 10.043
Thurlkill 10.145
EMBOSS 10.482
Sillero 10.218
Patrickios 9.838
IPC_peptide 10.365
IPC2_peptide 8.843
IPC2.peptide.svr19 8.646
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
13887
38
1803
257.2
28.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.389 ± 0.488
0.598 ± 0.109
6.207 ± 0.193
7.136 ± 0.504
3.953 ± 0.249
7.273 ± 0.362
1.858 ± 0.126
5.394 ± 0.167
6.38 ± 0.371
7.338 ± 0.287
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.859 ± 0.187
5.415 ± 0.258
4.623 ± 0.287
4.45 ± 0.319
4.652 ± 0.2
6.286 ± 0.331
6.027 ± 0.269
6.358 ± 0.215
1.455 ± 0.126
3.348 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here