Amino acid dipepetide frequency for Lactococcus phage ul36.k1t1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.731AlaAla: 4.731 ± 0.973
0.273AlaCys: 0.273 ± 0.189
4.094AlaAsp: 4.094 ± 0.637
4.003AlaGlu: 4.003 ± 0.781
2.729AlaPhe: 2.729 ± 0.518
4.549AlaGly: 4.549 ± 0.858
1.183AlaHis: 1.183 ± 0.296
5.277AlaIle: 5.277 ± 1.005
4.731AlaLys: 4.731 ± 0.553
7.096AlaLeu: 7.096 ± 1.245
1.365AlaMet: 1.365 ± 0.316
4.003AlaAsn: 4.003 ± 0.622
2.183AlaPro: 2.183 ± 0.435
3.093AlaGln: 3.093 ± 0.57
2.456AlaArg: 2.456 ± 0.536
3.73AlaSer: 3.73 ± 0.522
4.367AlaThr: 4.367 ± 0.84
4.094AlaVal: 4.094 ± 0.52
0.819AlaTrp: 0.819 ± 0.317
2.001AlaTyr: 2.001 ± 0.414
0.0AlaXaa: 0.0 ± 0.0
Cys
0.091CysAla: 0.091 ± 0.109
0.0CysCys: 0.0 ± 0.0
0.455CysAsp: 0.455 ± 0.192
0.637CysGlu: 0.637 ± 0.316
0.455CysPhe: 0.455 ± 0.189
0.546CysGly: 0.546 ± 0.26
0.455CysHis: 0.455 ± 0.352
0.546CysIle: 0.546 ± 0.261
0.455CysLys: 0.455 ± 0.229
0.182CysLeu: 0.182 ± 0.121
0.182CysMet: 0.182 ± 0.148
0.091CysAsn: 0.091 ± 0.1
0.091CysPro: 0.091 ± 0.093
0.091CysGln: 0.091 ± 0.088
0.091CysArg: 0.091 ± 0.085
0.273CysSer: 0.273 ± 0.152
0.273CysThr: 0.273 ± 0.197
0.0CysVal: 0.0 ± 0.0
0.091CysTrp: 0.091 ± 0.108
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
3.184AspAla: 3.184 ± 0.642
0.364AspCys: 0.364 ± 0.243
3.548AspAsp: 3.548 ± 0.658
4.731AspGlu: 4.731 ± 0.68
4.094AspPhe: 4.094 ± 0.645
5.822AspGly: 5.822 ± 1.334
0.273AspHis: 0.273 ± 0.148
4.185AspIle: 4.185 ± 0.608
5.913AspLys: 5.913 ± 0.765
4.185AspLeu: 4.185 ± 0.698
1.638AspMet: 1.638 ± 0.436
3.821AspAsn: 3.821 ± 0.443
1.274AspPro: 1.274 ± 0.445
0.819AspGln: 0.819 ± 0.285
2.365AspArg: 2.365 ± 0.397
4.731AspSer: 4.731 ± 0.718
3.093AspThr: 3.093 ± 0.54
3.912AspVal: 3.912 ± 0.607
0.91AspTrp: 0.91 ± 0.28
3.093AspTyr: 3.093 ± 0.502
0.0AspXaa: 0.0 ± 0.0
Glu
4.367GluAla: 4.367 ± 0.925
0.546GluCys: 0.546 ± 0.254
2.729GluAsp: 2.729 ± 0.451
6.55GluGlu: 6.55 ± 1.153
3.639GluPhe: 3.639 ± 0.62
2.274GluGly: 2.274 ± 0.461
1.001GluHis: 1.001 ± 0.304
5.731GluIle: 5.731 ± 0.825
7.46GluLys: 7.46 ± 1.239
7.278GluLeu: 7.278 ± 0.856
1.729GluMet: 1.729 ± 0.408
4.367GluAsn: 4.367 ± 0.87
1.82GluPro: 1.82 ± 0.521
3.275GluGln: 3.275 ± 0.575
2.911GluArg: 2.911 ± 0.659
3.912GluSer: 3.912 ± 0.592
4.731GluThr: 4.731 ± 0.734
5.095GluVal: 5.095 ± 0.784
1.365GluTrp: 1.365 ± 0.406
3.002GluTyr: 3.002 ± 0.521
0.0GluXaa: 0.0 ± 0.0
Phe
2.638PheAla: 2.638 ± 0.474
0.182PheCys: 0.182 ± 0.14
2.638PheAsp: 2.638 ± 0.568
4.276PheGlu: 4.276 ± 0.728
1.638PhePhe: 1.638 ± 0.438
2.183PheGly: 2.183 ± 0.316
0.364PheHis: 0.364 ± 0.17
3.366PheIle: 3.366 ± 0.589
3.821PheLys: 3.821 ± 0.478
1.91PheLeu: 1.91 ± 0.372
1.547PheMet: 1.547 ± 0.42
2.638PheAsn: 2.638 ± 0.387
1.183PhePro: 1.183 ± 0.321
1.183PheGln: 1.183 ± 0.313
1.547PheArg: 1.547 ± 0.413
3.093PheSer: 3.093 ± 0.599
2.911PheThr: 2.911 ± 0.486
3.093PheVal: 3.093 ± 0.576
0.455PheTrp: 0.455 ± 0.197
2.365PheTyr: 2.365 ± 0.537
0.0PheXaa: 0.0 ± 0.0
Gly
2.911GlyAla: 2.911 ± 0.601
0.364GlyCys: 0.364 ± 0.223
4.003GlyAsp: 4.003 ± 0.661
3.821GlyGlu: 3.821 ± 0.753
3.366GlyPhe: 3.366 ± 0.686
4.458GlyGly: 4.458 ± 0.64
0.91GlyHis: 0.91 ± 0.21
5.913GlyIle: 5.913 ± 0.821
5.459GlyLys: 5.459 ± 0.645
4.64GlyLeu: 4.64 ± 0.787
1.365GlyMet: 1.365 ± 0.391
3.639GlyAsn: 3.639 ± 0.47
1.183GlyPro: 1.183 ± 0.484
3.002GlyGln: 3.002 ± 0.526
2.183GlyArg: 2.183 ± 0.458
5.095GlySer: 5.095 ± 0.701
5.731GlyThr: 5.731 ± 0.946
3.912GlyVal: 3.912 ± 0.489
0.91GlyTrp: 0.91 ± 0.316
2.365GlyTyr: 2.365 ± 0.404
0.0GlyXaa: 0.0 ± 0.0
His
0.91HisAla: 0.91 ± 0.402
0.091HisCys: 0.091 ± 0.098
1.183HisAsp: 1.183 ± 0.358
1.183HisGlu: 1.183 ± 0.367
0.455HisPhe: 0.455 ± 0.213
1.001HisGly: 1.001 ± 0.32
0.273HisHis: 0.273 ± 0.159
1.001HisIle: 1.001 ± 0.296
0.728HisLys: 0.728 ± 0.248
1.001HisLeu: 1.001 ± 0.228
0.182HisMet: 0.182 ± 0.135
0.546HisAsn: 0.546 ± 0.241
0.364HisPro: 0.364 ± 0.215
0.273HisGln: 0.273 ± 0.145
0.364HisArg: 0.364 ± 0.157
0.91HisSer: 0.91 ± 0.348
0.637HisThr: 0.637 ± 0.202
0.91HisVal: 0.91 ± 0.371
0.091HisTrp: 0.091 ± 0.076
0.637HisTyr: 0.637 ± 0.288
0.0HisXaa: 0.0 ± 0.0
Ile
5.368IleAla: 5.368 ± 0.609
0.637IleCys: 0.637 ± 0.368
5.095IleAsp: 5.095 ± 0.611
5.368IleGlu: 5.368 ± 0.853
1.547IlePhe: 1.547 ± 0.418
5.186IleGly: 5.186 ± 0.776
0.91IleHis: 0.91 ± 0.438
5.731IleIle: 5.731 ± 1.358
6.914IleLys: 6.914 ± 0.758
5.277IleLeu: 5.277 ± 0.704
1.82IleMet: 1.82 ± 0.422
4.731IleAsn: 4.731 ± 0.653
3.184IlePro: 3.184 ± 0.671
3.184IleGln: 3.184 ± 0.563
2.638IleArg: 2.638 ± 0.437
5.549IleSer: 5.549 ± 0.713
4.458IleThr: 4.458 ± 0.799
3.821IleVal: 3.821 ± 0.689
0.364IleTrp: 0.364 ± 0.154
1.456IleTyr: 1.456 ± 0.283
0.0IleXaa: 0.0 ± 0.0
Lys
6.277LysAla: 6.277 ± 0.826
0.455LysCys: 0.455 ± 0.236
5.186LysAsp: 5.186 ± 0.721
7.369LysGlu: 7.369 ± 1.26
3.821LysPhe: 3.821 ± 0.748
4.822LysGly: 4.822 ± 0.536
0.728LysHis: 0.728 ± 0.293
6.095LysIle: 6.095 ± 0.752
7.915LysLys: 7.915 ± 1.263
7.005LysLeu: 7.005 ± 0.789
2.82LysMet: 2.82 ± 0.541
4.822LysAsn: 4.822 ± 0.694
2.456LysPro: 2.456 ± 0.439
4.367LysGln: 4.367 ± 0.782
2.456LysArg: 2.456 ± 0.453
5.186LysSer: 5.186 ± 0.87
4.458LysThr: 4.458 ± 0.582
5.277LysVal: 5.277 ± 0.589
1.729LysTrp: 1.729 ± 0.423
2.911LysTyr: 2.911 ± 0.572
0.0LysXaa: 0.0 ± 0.0
Leu
5.368LeuAla: 5.368 ± 0.671
0.273LeuCys: 0.273 ± 0.154
4.64LeuAsp: 4.64 ± 0.659
6.095LeuGlu: 6.095 ± 0.924
3.275LeuPhe: 3.275 ± 0.482
5.64LeuGly: 5.64 ± 0.849
0.546LeuHis: 0.546 ± 0.256
5.368LeuIle: 5.368 ± 1.007
5.731LeuLys: 5.731 ± 0.797
6.186LeuLeu: 6.186 ± 1.121
2.274LeuMet: 2.274 ± 0.444
3.457LeuAsn: 3.457 ± 0.549
4.276LeuPro: 4.276 ± 0.882
2.092LeuGln: 2.092 ± 0.395
2.729LeuArg: 2.729 ± 0.548
6.914LeuSer: 6.914 ± 0.825
5.549LeuThr: 5.549 ± 0.759
3.366LeuVal: 3.366 ± 0.676
1.274LeuTrp: 1.274 ± 0.326
2.001LeuTyr: 2.001 ± 0.564
0.0LeuXaa: 0.0 ± 0.0
Met
1.91MetAla: 1.91 ± 0.455
0.091MetCys: 0.091 ± 0.093
1.365MetAsp: 1.365 ± 0.405
1.91MetGlu: 1.91 ± 0.439
0.819MetPhe: 0.819 ± 0.309
1.274MetGly: 1.274 ± 0.354
0.273MetHis: 0.273 ± 0.136
1.456MetIle: 1.456 ± 0.408
2.092MetLys: 2.092 ± 0.56
1.274MetLeu: 1.274 ± 0.319
0.455MetMet: 0.455 ± 0.237
1.91MetAsn: 1.91 ± 0.433
0.455MetPro: 0.455 ± 0.192
0.91MetGln: 0.91 ± 0.315
1.001MetArg: 1.001 ± 0.273
1.91MetSer: 1.91 ± 0.392
2.638MetThr: 2.638 ± 0.45
0.91MetVal: 0.91 ± 0.255
0.273MetTrp: 0.273 ± 0.152
0.364MetTyr: 0.364 ± 0.196
0.0MetXaa: 0.0 ± 0.0
Asn
3.639AsnAla: 3.639 ± 0.675
0.182AsnCys: 0.182 ± 0.12
3.73AsnAsp: 3.73 ± 0.603
2.911AsnGlu: 2.911 ± 0.607
3.093AsnPhe: 3.093 ± 0.563
5.822AsnGly: 5.822 ± 0.952
0.91AsnHis: 0.91 ± 0.38
4.367AsnIle: 4.367 ± 0.518
4.276AsnLys: 4.276 ± 0.628
3.912AsnLeu: 3.912 ± 0.54
1.001AsnMet: 1.001 ± 0.325
3.912AsnAsn: 3.912 ± 0.676
2.456AsnPro: 2.456 ± 0.574
1.82AsnGln: 1.82 ± 0.427
2.092AsnArg: 2.092 ± 0.483
3.821AsnSer: 3.821 ± 0.672
2.547AsnThr: 2.547 ± 0.474
3.457AsnVal: 3.457 ± 0.552
0.728AsnTrp: 0.728 ± 0.237
3.093AsnTyr: 3.093 ± 0.503
0.0AsnXaa: 0.0 ± 0.0
Pro
1.82ProAla: 1.82 ± 0.437
0.091ProCys: 0.091 ± 0.104
2.183ProAsp: 2.183 ± 0.537
2.001ProGlu: 2.001 ± 0.419
1.001ProPhe: 1.001 ± 0.283
0.546ProGly: 0.546 ± 0.205
0.546ProHis: 0.546 ± 0.198
1.638ProIle: 1.638 ± 0.357
2.274ProLys: 2.274 ± 0.57
2.001ProLeu: 2.001 ± 0.518
1.001ProMet: 1.001 ± 0.275
1.547ProAsn: 1.547 ± 0.491
0.91ProPro: 0.91 ± 0.276
3.093ProGln: 3.093 ± 0.538
1.092ProArg: 1.092 ± 0.415
2.911ProSer: 2.911 ± 0.466
3.275ProThr: 3.275 ± 0.672
2.274ProVal: 2.274 ± 0.396
0.364ProTrp: 0.364 ± 0.164
1.274ProTyr: 1.274 ± 0.34
0.0ProXaa: 0.0 ± 0.0
Gln
3.821GlnAla: 3.821 ± 0.83
0.091GlnCys: 0.091 ± 0.082
1.729GlnAsp: 1.729 ± 0.383
3.821GlnGlu: 3.821 ± 0.653
1.547GlnPhe: 1.547 ± 0.31
2.82GlnGly: 2.82 ± 0.585
0.455GlnHis: 0.455 ± 0.2
3.093GlnIle: 3.093 ± 0.599
3.457GlnLys: 3.457 ± 0.51
3.457GlnLeu: 3.457 ± 0.566
1.274GlnMet: 1.274 ± 0.31
1.82GlnAsn: 1.82 ± 0.312
1.547GlnPro: 1.547 ± 0.403
2.274GlnGln: 2.274 ± 0.398
1.274GlnArg: 1.274 ± 0.398
2.365GlnSer: 2.365 ± 0.398
2.365GlnThr: 2.365 ± 0.423
2.729GlnVal: 2.729 ± 0.372
0.364GlnTrp: 0.364 ± 0.185
0.91GlnTyr: 0.91 ± 0.288
0.0GlnXaa: 0.0 ± 0.0
Arg
2.638ArgAla: 2.638 ± 0.531
0.364ArgCys: 0.364 ± 0.2
2.547ArgAsp: 2.547 ± 0.463
2.638ArgGlu: 2.638 ± 0.508
1.001ArgPhe: 1.001 ± 0.338
1.638ArgGly: 1.638 ± 0.524
0.546ArgHis: 0.546 ± 0.24
2.547ArgIle: 2.547 ± 0.487
3.73ArgLys: 3.73 ± 0.717
3.002ArgLeu: 3.002 ± 0.516
0.91ArgMet: 0.91 ± 0.296
1.547ArgAsn: 1.547 ± 0.412
0.819ArgPro: 0.819 ± 0.296
1.638ArgGln: 1.638 ± 0.397
1.82ArgArg: 1.82 ± 0.343
1.092ArgSer: 1.092 ± 0.339
1.365ArgThr: 1.365 ± 0.373
2.183ArgVal: 2.183 ± 0.41
0.546ArgTrp: 0.546 ± 0.224
1.456ArgTyr: 1.456 ± 0.411
0.0ArgXaa: 0.0 ± 0.0
Ser
4.276SerAla: 4.276 ± 0.595
0.273SerCys: 0.273 ± 0.143
5.277SerAsp: 5.277 ± 0.878
4.458SerGlu: 4.458 ± 0.792
3.73SerPhe: 3.73 ± 0.399
5.459SerGly: 5.459 ± 0.68
0.819SerHis: 0.819 ± 0.28
4.64SerIle: 4.64 ± 0.825
6.277SerLys: 6.277 ± 0.803
5.095SerLeu: 5.095 ± 0.612
0.91SerMet: 0.91 ± 0.276
5.368SerAsn: 5.368 ± 0.578
1.274SerPro: 1.274 ± 0.384
2.729SerGln: 2.729 ± 0.524
2.092SerArg: 2.092 ± 0.444
5.549SerSer: 5.549 ± 0.997
3.548SerThr: 3.548 ± 0.505
3.912SerVal: 3.912 ± 0.657
0.819SerTrp: 0.819 ± 0.31
3.366SerTyr: 3.366 ± 0.463
0.0SerXaa: 0.0 ± 0.0
Thr
5.095ThrAla: 5.095 ± 1.054
0.455ThrCys: 0.455 ± 0.256
3.639ThrAsp: 3.639 ± 0.717
4.185ThrGlu: 4.185 ± 0.682
1.91ThrPhe: 1.91 ± 0.511
5.095ThrGly: 5.095 ± 0.736
1.183ThrHis: 1.183 ± 0.301
4.367ThrIle: 4.367 ± 0.734
4.913ThrLys: 4.913 ± 0.857
5.095ThrLeu: 5.095 ± 0.796
0.728ThrMet: 0.728 ± 0.238
4.276ThrAsn: 4.276 ± 0.791
3.639ThrPro: 3.639 ± 0.645
2.456ThrGln: 2.456 ± 0.497
1.91ThrArg: 1.91 ± 0.414
4.185ThrSer: 4.185 ± 0.676
5.64ThrThr: 5.64 ± 0.98
5.004ThrVal: 5.004 ± 0.885
0.637ThrTrp: 0.637 ± 0.265
2.092ThrTyr: 2.092 ± 0.582
0.0ThrXaa: 0.0 ± 0.0
Val
4.276ValAla: 4.276 ± 0.581
0.182ValCys: 0.182 ± 0.149
4.458ValAsp: 4.458 ± 0.521
4.731ValGlu: 4.731 ± 0.659
2.092ValPhe: 2.092 ± 0.303
3.275ValGly: 3.275 ± 0.49
0.637ValHis: 0.637 ± 0.232
4.64ValIle: 4.64 ± 0.575
5.731ValLys: 5.731 ± 0.888
3.457ValLeu: 3.457 ± 0.669
1.092ValMet: 1.092 ± 0.306
3.093ValAsn: 3.093 ± 0.555
1.547ValPro: 1.547 ± 0.447
2.911ValGln: 2.911 ± 0.737
1.547ValArg: 1.547 ± 0.414
4.822ValSer: 4.822 ± 0.594
5.368ValThr: 5.368 ± 0.663
4.003ValVal: 4.003 ± 0.709
0.637ValTrp: 0.637 ± 0.246
2.001ValTyr: 2.001 ± 0.408
0.0ValXaa: 0.0 ± 0.0
Trp
1.001TrpAla: 1.001 ± 0.399
0.091TrpCys: 0.091 ± 0.085
0.819TrpAsp: 0.819 ± 0.294
0.819TrpGlu: 0.819 ± 0.301
0.637TrpPhe: 0.637 ± 0.239
0.455TrpGly: 0.455 ± 0.164
0.091TrpHis: 0.091 ± 0.084
0.728TrpIle: 0.728 ± 0.215
1.183TrpLys: 1.183 ± 0.32
1.456TrpLeu: 1.456 ± 0.359
0.273TrpMet: 0.273 ± 0.175
1.001TrpAsn: 1.001 ± 0.304
0.091TrpPro: 0.091 ± 0.102
0.637TrpGln: 0.637 ± 0.236
0.637TrpArg: 0.637 ± 0.243
0.91TrpSer: 0.91 ± 0.296
0.637TrpThr: 0.637 ± 0.307
0.819TrpVal: 0.819 ± 0.245
0.273TrpTrp: 0.273 ± 0.165
0.637TrpTyr: 0.637 ± 0.222
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.456TyrAla: 2.456 ± 0.534
0.0TyrCys: 0.0 ± 0.0
2.638TyrAsp: 2.638 ± 0.524
2.274TyrGlu: 2.274 ± 0.475
2.183TyrPhe: 2.183 ± 0.479
2.365TyrGly: 2.365 ± 0.581
0.637TyrHis: 0.637 ± 0.273
2.547TyrIle: 2.547 ± 0.535
3.093TyrLys: 3.093 ± 0.536
3.548TyrLeu: 3.548 ± 0.648
0.637TyrMet: 0.637 ± 0.232
1.183TyrAsn: 1.183 ± 0.301
1.092TyrPro: 1.092 ± 0.278
1.365TyrGln: 1.365 ± 0.31
0.91TyrArg: 0.91 ± 0.295
2.911TyrSer: 2.911 ± 0.593
2.911TyrThr: 2.911 ± 0.628
1.729TyrVal: 1.729 ± 0.396
0.546TyrTrp: 0.546 ± 0.23
1.82TyrTyr: 1.82 ± 0.425
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (10993 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski