Actinomyces sp. oral taxon 180 str. F0310

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces; unclassified Actinomyces; Actinomyces sp. oral taxon 180

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2070 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E6KRP5|E6KRP5_9ACTO ABC superfamily ATP binding cassette transporter ABC protein OS=Actinomyces sp. oral taxon 180 str. F0310 OX=888052 GN=thrB2 PE=4 SV=1
MM1 pKa = 7.88SIRR4 pKa = 11.84FLRR7 pKa = 11.84SSLRR11 pKa = 11.84AAPLVGATLAVSLLSLAGLAAYY33 pKa = 8.44TGGGEE38 pKa = 4.73AAGQPDD44 pKa = 4.62PSPIVYY50 pKa = 10.11EE51 pKa = 4.65DD52 pKa = 4.35DD53 pKa = 3.92SSLSIVAAFTDD64 pKa = 3.75MTSQLNGSVNTVAYY78 pKa = 8.0QQAYY82 pKa = 10.02RR83 pKa = 11.84GVTYY87 pKa = 10.59DD88 pKa = 3.08KK89 pKa = 10.47EE90 pKa = 4.56AYY92 pKa = 10.09VYY94 pKa = 10.35VPPTYY99 pKa = 11.0SPDD102 pKa = 3.19TPANVVYY109 pKa = 8.88LTHH112 pKa = 6.53GWQGSAAGLAEE123 pKa = 3.91GVVPVVDD130 pKa = 4.2EE131 pKa = 4.36LTASGQLSPTLVVFATYY148 pKa = 10.78YY149 pKa = 9.27PDD151 pKa = 3.68RR152 pKa = 11.84SFAGDD157 pKa = 4.08DD158 pKa = 3.76YY159 pKa = 11.86EE160 pKa = 6.92DD161 pKa = 5.11DD162 pKa = 4.06YY163 pKa = 11.97EE164 pKa = 4.89LNRR167 pKa = 11.84FFATTEE173 pKa = 3.28IDD175 pKa = 3.79TIIDD179 pKa = 3.65AVEE182 pKa = 3.84SRR184 pKa = 11.84YY185 pKa = 8.75ATYY188 pKa = 10.96AGGDD192 pKa = 3.41TSDD195 pKa = 4.57QSLQDD200 pKa = 3.69SRR202 pKa = 11.84THH204 pKa = 6.17RR205 pKa = 11.84AFGGFSMGATTTWDD219 pKa = 3.64VFSMRR224 pKa = 11.84PQYY227 pKa = 10.55FYY229 pKa = 11.05GYY231 pKa = 8.77MPMAGEE237 pKa = 4.04SWIGRR242 pKa = 11.84EE243 pKa = 3.48TDD245 pKa = 3.26ADD247 pKa = 3.44IDD249 pKa = 4.23QIAHH253 pKa = 7.21LIAAGAEE260 pKa = 4.01RR261 pKa = 11.84ADD263 pKa = 3.94YY264 pKa = 10.18GAQDD268 pKa = 3.57FRR270 pKa = 11.84ILASVGSDD278 pKa = 5.34DD279 pKa = 3.97PALWDD284 pKa = 3.7MSPQLEE290 pKa = 4.24QLQLDD295 pKa = 4.66YY296 pKa = 10.73PDD298 pKa = 5.32LMTPGSLQLWIDD310 pKa = 3.5EE311 pKa = 4.83GEE313 pKa = 4.19DD314 pKa = 3.16HH315 pKa = 7.65SMASIQNQVAHH326 pKa = 7.03DD327 pKa = 4.56LPRR330 pKa = 11.84LLPQAA335 pKa = 4.34

Molecular weight: 36.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E6KTB0|E6KTB0_9ACTO Uncharacterized protein (Fragment) OS=Actinomyces sp. oral taxon 180 str. F0310 OX=888052 GN=HMPREF9006_1462 PE=4 SV=1
MM1 pKa = 6.74EE2 pKa = 5.03TAPPTHH8 pKa = 6.95PPLLRR13 pKa = 11.84RR14 pKa = 11.84TSPSKK19 pKa = 10.86KK20 pKa = 8.66PWAAPTSSRR29 pKa = 11.84TVEE32 pKa = 4.23GLQQTRR38 pKa = 11.84PPPTRR43 pKa = 11.84TRR45 pKa = 11.84PQTQGRR51 pKa = 11.84LATAPGTQMAPVRR64 pKa = 11.84LIPTLSRR71 pKa = 11.84GTIPASARR79 pKa = 11.84TLTPRR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84TTPPTVRR93 pKa = 11.84PTTPLIRR100 pKa = 11.84RR101 pKa = 11.84ATATRR106 pKa = 11.84PRR108 pKa = 11.84PRR110 pKa = 11.84KK111 pKa = 8.01TSRR114 pKa = 11.84TSPRR118 pKa = 11.84RR119 pKa = 11.84ISPTRR124 pKa = 11.84KK125 pKa = 5.37TTPTASPIRR134 pKa = 11.84TRR136 pKa = 11.84PTRR139 pKa = 11.84TSPTRR144 pKa = 11.84IRR146 pKa = 11.84PSAQTPIRR154 pKa = 11.84RR155 pKa = 11.84RR156 pKa = 11.84RR157 pKa = 11.84TTPPTARR164 pKa = 11.84PTTPLTRR171 pKa = 11.84RR172 pKa = 11.84ATVTRR177 pKa = 11.84PRR179 pKa = 11.84PTNPRR184 pKa = 11.84RR185 pKa = 11.84TSPTRR190 pKa = 11.84KK191 pKa = 5.6TTPHH195 pKa = 5.57TTTATSTPRR204 pKa = 11.84TPTTSTPPRR213 pKa = 11.84TARR216 pKa = 11.84PTPTPSPTTIRR227 pKa = 11.84PSTRR231 pKa = 11.84TPIRR235 pKa = 11.84RR236 pKa = 11.84RR237 pKa = 11.84RR238 pKa = 11.84ITLPTARR245 pKa = 11.84PTTLLIRR252 pKa = 11.84RR253 pKa = 11.84TAATSPRR260 pKa = 11.84PTRR263 pKa = 11.84ARR265 pKa = 11.84QISPPRR271 pKa = 11.84TSLTRR276 pKa = 11.84QTTPHH281 pKa = 5.81TTTTPSTPRR290 pKa = 11.84TPTARR295 pKa = 11.84PPRR298 pKa = 11.84RR299 pKa = 11.84TPTRR303 pKa = 11.84SRR305 pKa = 11.84RR306 pKa = 11.84TTPPTTRR313 pKa = 11.84QTTPTIRR320 pKa = 11.84RR321 pKa = 11.84AAATSPRR328 pKa = 11.84PTRR331 pKa = 11.84PRR333 pKa = 11.84QISPPRR339 pKa = 11.84TSLTRR344 pKa = 11.84QTTPHH349 pKa = 5.81TTTTPSTPRR358 pKa = 11.84TPTATPSPTRR368 pKa = 11.84TPTTRR373 pKa = 11.84STPRR377 pKa = 11.84KK378 pKa = 7.72RR379 pKa = 11.84THH381 pKa = 6.88PSTTRR386 pKa = 11.84TRR388 pKa = 11.84TSRR391 pKa = 11.84RR392 pKa = 11.84VPTQQQTSMPATGGPTTLRR411 pKa = 11.84MPGRR415 pKa = 11.84TATRR419 pKa = 11.84KK420 pKa = 9.74RR421 pKa = 11.84QVHH424 pKa = 5.83LPKK427 pKa = 10.24TPTASIPMILLEE439 pKa = 4.31RR440 pKa = 11.84TASPSPLTATPAIHH454 pKa = 7.03RR455 pKa = 11.84AMSTARR461 pKa = 11.84STEE464 pKa = 3.66IVTPTAIRR472 pKa = 11.84ASTKK476 pKa = 9.93ALRR479 pKa = 11.84VTKK482 pKa = 10.11VRR484 pKa = 11.84RR485 pKa = 11.84KK486 pKa = 9.62RR487 pKa = 11.84STRR490 pKa = 11.84ICTITHH496 pKa = 5.04TMARR500 pKa = 3.87

Molecular weight: 55.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2070

0

2070

692431

37

3083

334.5

35.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.841 ± 0.077

0.819 ± 0.017

5.973 ± 0.043

5.735 ± 0.053

2.879 ± 0.037

8.854 ± 0.047

2.008 ± 0.023

4.653 ± 0.04

2.393 ± 0.035

9.278 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.201 ± 0.024

2.248 ± 0.033

5.33 ± 0.048

2.912 ± 0.03

7.147 ± 0.064

6.629 ± 0.049

6.023 ± 0.05

8.36 ± 0.053

1.517 ± 0.026

2.202 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski