Sheep faeces associated smacovirus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus; Porprismacovirus sheas3

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A168MFI8|A0A168MFI8_9VIRU Replication associated protein OS=Sheep faeces associated smacovirus 3 OX=1843758 PE=4 SV=1
MM1 pKa = 7.16VFVKK5 pKa = 10.43VRR7 pKa = 11.84EE8 pKa = 4.3TYY10 pKa = 10.49DD11 pKa = 3.1LHH13 pKa = 5.18TLKK16 pKa = 11.12GKK18 pKa = 9.6MSVIGIHH25 pKa = 5.87TPSDD29 pKa = 3.73SIIARR34 pKa = 11.84NYY36 pKa = 9.5PGLLMQCKK44 pKa = 9.96AYY46 pKa = 10.43RR47 pKa = 11.84PVKK50 pKa = 10.41ADD52 pKa = 3.1VRR54 pKa = 11.84LACASVLPLDD64 pKa = 4.08PQGVGTTADD73 pKa = 3.49DD74 pKa = 3.77VAPEE78 pKa = 4.34DD79 pKa = 3.68VFNPILYY86 pKa = 9.58KK87 pKa = 10.71ALSNFGMSQIDD98 pKa = 3.84AYY100 pKa = 11.06VNSAGYY106 pKa = 10.09DD107 pKa = 3.38IIGNTLDD114 pKa = 4.02ASNTGIDD121 pKa = 3.44ATDD124 pKa = 4.62DD125 pKa = 3.34FDD127 pKa = 5.92LYY129 pKa = 11.44YY130 pKa = 11.28GLLSQTHH137 pKa = 5.37EE138 pKa = 4.23WKK140 pKa = 10.26HH141 pKa = 6.12ANPQQGLMMNDD152 pKa = 4.21LVPLVYY158 pKa = 9.02EE159 pKa = 5.03TYY161 pKa = 10.85QSIGDD166 pKa = 3.9NGRR169 pKa = 11.84GGADD173 pKa = 3.45NPHH176 pKa = 6.03VAIKK180 pKa = 10.35PDD182 pKa = 3.49DD183 pKa = 4.53TIPTGQIGNISVQTFRR199 pKa = 11.84GKK201 pKa = 10.06AHH203 pKa = 7.72PIPFLNCTVPVANSGNVDD221 pKa = 2.94TRR223 pKa = 11.84QNGFIGSGYY232 pKa = 9.75PNAQVGIPAPKK243 pKa = 9.31IYY245 pKa = 10.23CAAILVPPSRR255 pKa = 11.84LHH257 pKa = 5.42QLFFRR262 pKa = 11.84MVCEE266 pKa = 3.97WTLEE270 pKa = 3.86FSMIRR275 pKa = 11.84PLGEE279 pKa = 3.63ITSWAGLKK287 pKa = 10.46QMGSTSHH294 pKa = 6.46FMSYY298 pKa = 10.59DD299 pKa = 3.36YY300 pKa = 11.16SAEE303 pKa = 4.16SKK305 pKa = 10.02DD306 pKa = 3.71TVFKK310 pKa = 11.06DD311 pKa = 3.29VTDD314 pKa = 4.23LVDD317 pKa = 3.25VTQDD321 pKa = 3.15SGIKK325 pKa = 10.09KK326 pKa = 10.23VMM328 pKa = 3.67

Molecular weight: 35.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A168MFI8|A0A168MFI8_9VIRU Replication associated protein OS=Sheep faeces associated smacovirus 3 OX=1843758 PE=4 SV=1
MM1 pKa = 7.31LTMPWTGQSKK11 pKa = 10.5RR12 pKa = 11.84MINLYY17 pKa = 8.17ITRR20 pKa = 11.84NDD22 pKa = 2.59IHH24 pKa = 6.41EE25 pKa = 4.53WIIGYY30 pKa = 8.82EE31 pKa = 4.09VGRR34 pKa = 11.84DD35 pKa = 3.96GYY37 pKa = 10.66RR38 pKa = 11.84HH39 pKa = 4.43IHH41 pKa = 4.77VRR43 pKa = 11.84FNGRR47 pKa = 11.84GDD49 pKa = 3.44FGDD52 pKa = 3.57VQRR55 pKa = 11.84AFPGAHH61 pKa = 6.06IEE63 pKa = 4.18EE64 pKa = 4.85GTTMEE69 pKa = 4.18TEE71 pKa = 4.11YY72 pKa = 10.79EE73 pKa = 4.2KK74 pKa = 11.12KK75 pKa = 10.24DD76 pKa = 3.43GHH78 pKa = 5.69FVSYY82 pKa = 10.57EE83 pKa = 3.82DD84 pKa = 3.66SPDD87 pKa = 3.37VLRR90 pKa = 11.84CRR92 pKa = 11.84FGKK95 pKa = 10.44LRR97 pKa = 11.84DD98 pKa = 3.47HH99 pKa = 6.13QRR101 pKa = 11.84RR102 pKa = 11.84VVKK105 pKa = 10.47LLEE108 pKa = 4.33KK109 pKa = 10.65QSDD112 pKa = 3.34RR113 pKa = 11.84GILCWYY119 pKa = 9.96DD120 pKa = 3.22EE121 pKa = 4.35TGSIGKK127 pKa = 9.51SFTCRR132 pKa = 11.84HH133 pKa = 4.44LVEE136 pKa = 4.39RR137 pKa = 11.84RR138 pKa = 11.84RR139 pKa = 11.84AYY141 pKa = 9.74YY142 pKa = 10.16VPPTVDD148 pKa = 2.86NVKK151 pKa = 10.64QIIQWVCSGYY161 pKa = 9.86QRR163 pKa = 11.84QRR165 pKa = 11.84YY166 pKa = 8.83LIIDD170 pKa = 4.1IPRR173 pKa = 11.84SAEE176 pKa = 3.97WNKK179 pKa = 10.73SLYY182 pKa = 9.85TGLEE186 pKa = 4.07AIKK189 pKa = 10.75DD190 pKa = 3.53GLIYY194 pKa = 9.15DD195 pKa = 3.92TRR197 pKa = 11.84YY198 pKa = 9.47SAKK201 pKa = 10.33LRR203 pKa = 11.84DD204 pKa = 3.18IWGVKK209 pKa = 9.72ILVLTNSLPNLDD221 pKa = 5.19ALSQDD226 pKa = 2.08RR227 pKa = 11.84WMIINRR233 pKa = 11.84DD234 pKa = 3.59GKK236 pKa = 10.45DD237 pKa = 3.12VTEE240 pKa = 4.16WARR243 pKa = 11.84IEE245 pKa = 4.31SKK247 pKa = 10.66KK248 pKa = 9.79RR249 pKa = 11.84AKK251 pKa = 10.26KK252 pKa = 10.37KK253 pKa = 10.27ADD255 pKa = 3.23EE256 pKa = 4.53GGKK259 pKa = 10.23KK260 pKa = 8.26KK261 pKa = 9.0TKK263 pKa = 10.11KK264 pKa = 10.35SEE266 pKa = 3.89EE267 pKa = 3.94

Molecular weight: 31.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

595

267

328

297.5

33.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.546 ± 1.161

1.513 ± 0.009

7.731 ± 0.155

4.37 ± 1.529

3.025 ± 0.502

8.067 ± 0.13

2.521 ± 0.065

7.227 ± 0.411

6.387 ± 1.436

7.059 ± 0.205

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.526

4.034 ± 0.669

4.706 ± 1.343

3.866 ± 0.319

6.05 ± 2.377

6.05 ± 0.52

5.882 ± 0.412

6.891 ± 0.82

1.849 ± 0.74

4.538 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski