Vibrio phage VBM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4PML2|M4PML2_9CAUD Uncharacterized protein OS=Vibrio phage VBM1 OX=754074 GN=VPGG_00020 PE=4 SV=1
MM1 pKa = 7.69VNDD4 pKa = 4.51CDD6 pKa = 3.51MPAPYY11 pKa = 9.75RR12 pKa = 11.84YY13 pKa = 8.11TPDD16 pKa = 3.03EE17 pKa = 4.43VEE19 pKa = 3.91WMIEE23 pKa = 3.58NSIPMLRR30 pKa = 11.84WFDD33 pKa = 4.2EE34 pKa = 4.0YY35 pKa = 11.75DD36 pKa = 3.25LAYY39 pKa = 9.36PKK41 pKa = 10.48CLEE44 pKa = 4.13SAKK47 pKa = 10.88SGGGQQ52 pKa = 2.98

Molecular weight: 6.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4PMP1|M4PMP1_9CAUD Uncharacterized protein OS=Vibrio phage VBM1 OX=754074 GN=VPGG_00050 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.12APNGATHH9 pKa = 6.64KK10 pKa = 10.2CGNVFYY16 pKa = 10.96KK17 pKa = 10.3IGKK20 pKa = 8.68KK21 pKa = 10.35KK22 pKa = 10.46NFKK25 pKa = 10.18KK26 pKa = 10.89YY27 pKa = 8.96YY28 pKa = 10.14DD29 pKa = 4.5SVFFWNGQRR38 pKa = 11.84WEE40 pKa = 4.16RR41 pKa = 11.84TFCFPEE47 pKa = 3.91AQKK50 pKa = 10.07MVRR53 pKa = 11.84VV54 pKa = 3.9

Molecular weight: 6.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12091

52

941

215.9

24.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.691 ± 0.385

1.009 ± 0.129

6.443 ± 0.253

7.344 ± 0.505

4.334 ± 0.228

6.898 ± 0.291

1.464 ± 0.134

6.377 ± 0.186

7.146 ± 0.437

8.064 ± 0.209

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.151

5.194 ± 0.158

3.532 ± 0.177

3.937 ± 0.149

4.243 ± 0.231

7.948 ± 0.302

5.823 ± 0.361

6.509 ± 0.309

1.224 ± 0.119

3.457 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski