Klebsiella phage SopranoGao
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A248SL37|A0A248SL37_9CAUD Uncharacterized protein OS=Klebsiella phage SopranoGao OX=2026944 GN=SopranoGao_19 PE=4 SV=1
MM1 pKa = 6.89 ITIPVTGYY9 pKa = 11.08 AIVLACSINAGTPDD23 pKa = 5.28 CIEE26 pKa = 3.97 QLATGYY32 pKa = 10.03 VWANAQRR39 pKa = 11.84 CQEE42 pKa = 4.24 EE43 pKa = 4.58 LDD45 pKa = 3.59 HH46 pKa = 7.01 AKK48 pKa = 10.22 IPGAQCEE55 pKa = 4.48 SLSGITVSDD64 pKa = 4.05 EE65 pKa = 4.22 GTGPALARR73 pKa = 11.84 MIDD76 pKa = 3.63 VEE78 pKa = 4.68 GMM80 pKa = 3.32
Molecular weight: 8.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.975
IPC2_protein 4.19
IPC_protein 4.012
Toseland 3.859
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.884
Rodwell 3.859
Grimsley 3.77
Solomon 3.961
Lehninger 3.91
Nozaki 4.113
DTASelect 4.24
Thurlkill 3.897
EMBOSS 3.897
Sillero 4.126
Patrickios 0.401
IPC_peptide 3.961
IPC2_peptide 4.113
IPC2.peptide.svr19 4.034
Protein with the highest isoelectric point:
>tr|A0A248SKY6|A0A248SKY6_9CAUD Uncharacterized protein OS=Klebsiella phage SopranoGao OX=2026944 GN=SopranoGao_62 PE=4 SV=1
MM1 pKa = 7.64 SKK3 pKa = 10.5 RR4 pKa = 11.84 DD5 pKa = 3.41 IKK7 pKa = 10.65 AKK9 pKa = 9.63 RR10 pKa = 11.84 WARR13 pKa = 11.84 RR14 pKa = 11.84 QPPEE18 pKa = 4.37 PYY20 pKa = 10.17 DD21 pKa = 3.73 GVTIWFHH28 pKa = 4.06 EE29 pKa = 4.65 QIRR32 pKa = 11.84 IMGDD36 pKa = 2.68 GRR38 pKa = 11.84 RR39 pKa = 11.84 QFRR42 pKa = 11.84 DD43 pKa = 3.01 RR44 pKa = 11.84 LAGIRR49 pKa = 11.84 VEE51 pKa = 4.0 RR52 pKa = 11.84 QPWSEE57 pKa = 3.71 WRR59 pKa = 11.84 DD60 pKa = 3.6 CC61 pKa = 4.46
Molecular weight: 7.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 9.531
IPC_protein 10.643
Toseland 10.891
ProMoST 10.906
Dawson 10.935
Bjellqvist 10.745
Wikipedia 11.242
Rodwell 10.921
Grimsley 10.965
Solomon 11.213
Lehninger 11.155
Nozaki 10.877
DTASelect 10.745
Thurlkill 10.877
EMBOSS 11.33
Sillero 10.891
Patrickios 10.716
IPC_peptide 11.213
IPC2_peptide 10.014
IPC2.peptide.svr19 8.909
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
77
0
77
19201
43
4369
249.4
27.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.833 ± 0.379
0.823 ± 0.201
6.161 ± 0.191
6.963 ± 0.423
3.109 ± 0.15
7.63 ± 0.331
1.703 ± 0.184
4.968 ± 0.252
5.479 ± 0.272
7.474 ± 0.191
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.651 ± 0.145
3.906 ± 0.171
4.724 ± 0.21
5.224 ± 0.399
6.562 ± 0.304
5.593 ± 0.244
5.593 ± 0.224
5.807 ± 0.267
1.823 ± 0.209
2.974 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here