Faecalibacterium phage FP_Toutatis
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K9V414|A0A2K9V414_9CAUD Uncharacterized protein OS=Faecalibacterium phage FP_Toutatis OX=2070187 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 10.28 YY3 pKa = 10.43 DD4 pKa = 3.54 EE5 pKa = 4.54 RR6 pKa = 11.84 TCKK9 pKa = 10.46 FNMDD13 pKa = 4.13 TGCVEE18 pKa = 3.92 LLLRR22 pKa = 11.84 DD23 pKa = 3.4 GRR25 pKa = 11.84 MISIDD30 pKa = 3.45 CTGVEE35 pKa = 4.03 NALDD39 pKa = 3.44 VTIAQRR45 pKa = 11.84 SEE47 pKa = 3.88 LDD49 pKa = 3.27 YY50 pKa = 11.32 LIYY53 pKa = 10.51 NDD55 pKa = 4.02 PLGYY59 pKa = 10.65 ADD61 pKa = 6.36 LILNGDD67 pKa = 3.56 PEE69 pKa = 5.91 GYY71 pKa = 10.0 LKK73 pKa = 10.88 NVTGSHH79 pKa = 5.94 GLEE82 pKa = 4.41 DD83 pKa = 3.48
Molecular weight: 9.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.867
IPC2_protein 4.151
IPC_protein 4.075
Toseland 3.872
ProMoST 4.151
Dawson 4.062
Bjellqvist 4.266
Wikipedia 3.999
Rodwell 3.91
Grimsley 3.783
Solomon 4.05
Lehninger 4.012
Nozaki 4.177
DTASelect 4.406
Thurlkill 3.923
EMBOSS 4.012
Sillero 4.19
Patrickios 1.964
IPC_peptide 4.05
IPC2_peptide 4.177
IPC2.peptide.svr19 4.109
Protein with the highest isoelectric point:
>tr|A0A2K9V3Z1|A0A2K9V3Z1_9CAUD Uncharacterized protein OS=Faecalibacterium phage FP_Toutatis OX=2070187 PE=4 SV=1
MM1 pKa = 7.66 AKK3 pKa = 10.14 AIYY6 pKa = 9.55 DD7 pKa = 3.66 VQVPNIGEE15 pKa = 4.07 VEE17 pKa = 4.03 RR18 pKa = 11.84 ALGDD22 pKa = 3.65 MRR24 pKa = 11.84 DD25 pKa = 3.56 KK26 pKa = 11.14 APRR29 pKa = 11.84 AMKK32 pKa = 10.48 NAVNQTATRR41 pKa = 11.84 AKK43 pKa = 11.17 NMMIRR48 pKa = 11.84 QARR51 pKa = 11.84 LRR53 pKa = 11.84 YY54 pKa = 9.02 AVNSAGRR61 pKa = 11.84 RR62 pKa = 11.84 HH63 pKa = 6.19 LNALKK68 pKa = 10.29 VRR70 pKa = 11.84 NRR72 pKa = 11.84 ATTQNPTAEE81 pKa = 4.27 IFISSRR87 pKa = 11.84 RR88 pKa = 11.84 NDD90 pKa = 3.51 LGDD93 pKa = 3.62 FQSNPSVPHH102 pKa = 6.12 MGRR105 pKa = 11.84 DD106 pKa = 3.29 WVLSPEE112 pKa = 4.17 FHH114 pKa = 6.81 TSRR117 pKa = 11.84 VLKK120 pKa = 9.88 KK121 pKa = 10.79 SPMEE125 pKa = 4.2 ALTGGHH131 pKa = 5.89 TALGQASKK139 pKa = 11.23 GFLVKK144 pKa = 10.29 FDD146 pKa = 4.0 SGHH149 pKa = 6.7 VGMVQRR155 pKa = 11.84 LIGRR159 pKa = 11.84 PAANPKK165 pKa = 6.82 STRR168 pKa = 11.84 WRR170 pKa = 11.84 SKK172 pKa = 9.78 NGVVEE177 pKa = 4.29 KK178 pKa = 10.07 LYY180 pKa = 10.91 TMSSPSASAMHH191 pKa = 5.04 STVWRR196 pKa = 11.84 EE197 pKa = 3.69 EE198 pKa = 3.9 VEE200 pKa = 4.01 PDD202 pKa = 4.06 SEE204 pKa = 5.29 IILQEE209 pKa = 4.01 RR210 pKa = 11.84 LQHH213 pKa = 5.62 EE214 pKa = 4.49 VSKK217 pKa = 10.92 ILTQAGRR224 pKa = 11.84 KK225 pKa = 6.94 TKK227 pKa = 10.65
Molecular weight: 25.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.692
IPC_protein 10.423
Toseland 10.95
ProMoST 10.672
Dawson 10.994
Bjellqvist 10.687
Wikipedia 11.213
Rodwell 11.242
Grimsley 11.023
Solomon 11.155
Lehninger 11.125
Nozaki 10.906
DTASelect 10.687
Thurlkill 10.921
EMBOSS 11.345
Sillero 10.935
Patrickios 10.965
IPC_peptide 11.169
IPC2_peptide 9.341
IPC2.peptide.svr19 8.827
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
89
0
89
16100
35
1167
180.9
20.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.28 ± 0.406
1.466 ± 0.137
6.385 ± 0.204
7.112 ± 0.396
3.224 ± 0.124
7.242 ± 0.46
1.683 ± 0.156
5.304 ± 0.182
6.938 ± 0.265
7.267 ± 0.248
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.783 ± 0.142
4.273 ± 0.234
3.733 ± 0.218
3.441 ± 0.157
5.273 ± 0.432
5.292 ± 0.289
6.385 ± 0.26
6.988 ± 0.246
1.317 ± 0.097
3.615 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here