Lepus americanus faeces associated genomovirus SHP111

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; unclassified Genomoviridae

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5CI37|A0A2Z5CI37_9VIRU Replication associated protein OS=Lepus americanus faeces associated genomovirus SHP111 OX=2219118 PE=3 SV=1
MM1 pKa = 7.71PFSFNARR8 pKa = 11.84YY9 pKa = 9.01FLVTYY14 pKa = 9.33SHH16 pKa = 7.15VDD18 pKa = 3.36LLDD21 pKa = 3.63PFAIVDD27 pKa = 3.62HH28 pKa = 6.61FGSLGAEE35 pKa = 4.0CIVARR40 pKa = 11.84EE41 pKa = 4.39SYY43 pKa = 10.9NSVLGIHH50 pKa = 5.55YY51 pKa = 10.32HH52 pKa = 5.22VFADD56 pKa = 4.63FGRR59 pKa = 11.84KK60 pKa = 8.2FRR62 pKa = 11.84SRR64 pKa = 11.84RR65 pKa = 11.84ADD67 pKa = 3.28VFDD70 pKa = 3.48VGGFHH75 pKa = 7.47PNIAPSRR82 pKa = 11.84GTPEE86 pKa = 4.45AGFDD90 pKa = 3.64YY91 pKa = 9.31ATKK94 pKa = 10.76DD95 pKa = 3.18GDD97 pKa = 3.7IVAGGLEE104 pKa = 4.36RR105 pKa = 11.84PCRR108 pKa = 11.84DD109 pKa = 2.99GTLSRR114 pKa = 11.84SAKK117 pKa = 8.06WAQIVDD123 pKa = 3.27AEE125 pKa = 4.52TRR127 pKa = 11.84DD128 pKa = 3.3EE129 pKa = 4.76FYY131 pKa = 10.9RR132 pKa = 11.84LCEE135 pKa = 3.91EE136 pKa = 5.02LDD138 pKa = 3.68PEE140 pKa = 4.71RR141 pKa = 11.84LVCSFGQIQKK151 pKa = 10.02FADD154 pKa = 3.08WKK156 pKa = 10.52FAVTPAPYY164 pKa = 7.46TTPDD168 pKa = 3.31GVFDD172 pKa = 4.31LANYY176 pKa = 10.13GDD178 pKa = 4.19LEE180 pKa = 4.25EE181 pKa = 4.86SKK183 pKa = 11.16SLVLYY188 pKa = 10.53GPSRR192 pKa = 11.84LGKK195 pKa = 7.29TVWSRR200 pKa = 11.84SLGEE204 pKa = 3.83HH205 pKa = 7.01LYY207 pKa = 10.97FGGIFSARR215 pKa = 11.84DD216 pKa = 3.36LDD218 pKa = 4.32RR219 pKa = 11.84DD220 pKa = 3.87DD221 pKa = 3.88VKK223 pKa = 11.41YY224 pKa = 11.03AVFDD228 pKa = 5.22DD229 pKa = 3.44IAGGIKK235 pKa = 10.03FFPRR239 pKa = 11.84FKK241 pKa = 10.79DD242 pKa = 3.12WLGCQMEE249 pKa = 4.46FMVKK253 pKa = 9.27QLYY256 pKa = 9.95RR257 pKa = 11.84DD258 pKa = 3.6PQLFRR263 pKa = 11.84WGRR266 pKa = 11.84PCIWVANTDD275 pKa = 3.19PRR277 pKa = 11.84LDD279 pKa = 3.64MTHH282 pKa = 7.44DD283 pKa = 3.41DD284 pKa = 4.56VMWLEE289 pKa = 4.14ANCIFVEE296 pKa = 3.84ITEE299 pKa = 4.91IIFHH303 pKa = 6.98ANTEE307 pKa = 4.15

Molecular weight: 35.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5CI37|A0A2Z5CI37_9VIRU Replication associated protein OS=Lepus americanus faeces associated genomovirus SHP111 OX=2219118 PE=3 SV=1
MM1 pKa = 7.78AYY3 pKa = 10.26ARR5 pKa = 11.84RR6 pKa = 11.84SSARR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84YY13 pKa = 8.36PARR16 pKa = 11.84KK17 pKa = 8.3SGSRR21 pKa = 11.84KK22 pKa = 9.17RR23 pKa = 11.84QVSTKK28 pKa = 9.99KK29 pKa = 10.1RR30 pKa = 11.84PAYY33 pKa = 9.6RR34 pKa = 11.84KK35 pKa = 9.49KK36 pKa = 10.35SRR38 pKa = 11.84PMSKK42 pKa = 10.11KK43 pKa = 9.96RR44 pKa = 11.84ILNVTSRR51 pKa = 11.84KK52 pKa = 9.78KK53 pKa = 10.32RR54 pKa = 11.84DD55 pKa = 3.29TMLSTFQGGSASTPGQGAYY74 pKa = 6.9TTFANNAITIFPWVATGRR92 pKa = 11.84DD93 pKa = 3.18NSLIQGGAQAGTAFDD108 pKa = 3.55SSTRR112 pKa = 11.84SATTCYY118 pKa = 9.89MRR120 pKa = 11.84GLKK123 pKa = 9.55EE124 pKa = 4.23AIEE127 pKa = 4.37IQTSTGLPWQWRR139 pKa = 11.84RR140 pKa = 11.84VCFTTKK146 pKa = 10.73DD147 pKa = 3.56PAGTLATGNGGISGVFEE164 pKa = 4.17NSNGFQRR171 pKa = 11.84LVYY174 pKa = 10.03NVTSASTSDD183 pKa = 3.28TLALNTLTAILFKK196 pKa = 11.13GRR198 pKa = 11.84INVDD202 pKa = 2.22WFDD205 pKa = 4.11YY206 pKa = 7.84MTAPLDD212 pKa = 3.75TLRR215 pKa = 11.84VTVKK219 pKa = 10.1FDD221 pKa = 3.27RR222 pKa = 11.84TRR224 pKa = 11.84TIASGNQSGAIRR236 pKa = 11.84KK237 pKa = 8.78FNLWHH242 pKa = 6.75PMNKK246 pKa = 9.6NLVYY250 pKa = 10.85DD251 pKa = 4.21DD252 pKa = 5.44DD253 pKa = 4.42EE254 pKa = 5.48VGGAEE259 pKa = 3.86NASYY263 pKa = 11.34YY264 pKa = 10.9SVASKK269 pKa = 11.13AGMGDD274 pKa = 3.72YY275 pKa = 10.76YY276 pKa = 10.83VIDD279 pKa = 4.19MIVPGKK285 pKa = 10.72GGTSSDD291 pKa = 3.25GFSFEE296 pKa = 4.4PQATLYY302 pKa = 9.19WHH304 pKa = 6.98EE305 pKa = 4.28KK306 pKa = 9.3

Molecular weight: 33.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

613

306

307

306.5

34.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.32 ± 0.549

1.468 ± 0.538

7.178 ± 1.503

3.915 ± 1.075

6.362 ± 1.396

8.483 ± 0.441

1.631 ± 0.646

4.568 ± 0.211

4.894 ± 0.869

6.362 ± 0.749

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.121 ± 0.326

3.589 ± 0.867

4.241 ± 0.427

2.61 ± 0.435

7.667 ± 0.332

7.341 ± 1.411

7.178 ± 1.95

5.873 ± 0.641

2.121 ± 0.106

4.078 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski