Coffea canephora (Robusta coffee)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Gentianales; Rubiaceae; Ixoroideae;

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25401 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A068VAB2|A0A068VAB2_COFCA Uncharacterized protein OS=Coffea canephora OX=49390 GN=GSCOC_T00001682001 PE=4 SV=1
MM1 pKa = 7.18AAVYY5 pKa = 10.63SFVDD9 pKa = 3.45TATDD13 pKa = 3.56PFNNSDD19 pKa = 4.05LMEE22 pKa = 4.38SQQQYY27 pKa = 7.42MQAIALPANFVSPWDD42 pKa = 4.76DD43 pKa = 3.17EE44 pKa = 4.37DD45 pKa = 4.52HH46 pKa = 7.22PEE48 pKa = 3.93NFIPGPEE55 pKa = 4.02DD56 pKa = 3.05QYY58 pKa = 11.46RR59 pKa = 11.84PQSIQEE65 pKa = 3.95SWEE68 pKa = 3.82EE69 pKa = 5.41DD70 pKa = 2.93EE71 pKa = 5.57DD72 pKa = 4.07QPEE75 pKa = 4.74NIPPPNSIQYY85 pKa = 9.24GGHH88 pKa = 5.7WDD90 pKa = 3.25IPQVYY95 pKa = 10.08DD96 pKa = 4.04EE97 pKa = 5.13YY98 pKa = 11.54DD99 pKa = 3.76DD100 pKa = 6.0DD101 pKa = 5.05GEE103 pKa = 4.47PDD105 pKa = 3.53EE106 pKa = 5.01EE107 pKa = 4.42FVDD110 pKa = 3.74ILGEE114 pKa = 3.9LAEE117 pKa = 4.54LNGEE121 pKa = 3.78IVTFGNEE128 pKa = 3.59LGVILGFDD136 pKa = 3.36VLLDD140 pKa = 3.52QRR142 pKa = 11.84SNLSQEE148 pKa = 4.4IIAEE152 pKa = 4.0HH153 pKa = 6.22LQTLKK158 pKa = 10.54YY159 pKa = 10.21QKK161 pKa = 9.99LAKK164 pKa = 9.67QDD166 pKa = 3.51EE167 pKa = 4.82DD168 pKa = 3.78EE169 pKa = 5.42DD170 pKa = 4.43EE171 pKa = 4.33VDD173 pKa = 4.17EE174 pKa = 5.45LCVICQDD181 pKa = 3.6EE182 pKa = 4.75FKK184 pKa = 10.66SGEE187 pKa = 3.88MLGTLGCRR195 pKa = 11.84HH196 pKa = 6.21EE197 pKa = 4.38FHH199 pKa = 7.39ADD201 pKa = 3.75CITRR205 pKa = 11.84WLQVKK210 pKa = 8.16NVCPICKK217 pKa = 9.79RR218 pKa = 11.84MAVHH222 pKa = 5.98HH223 pKa = 5.61QEE225 pKa = 3.69II226 pKa = 4.17

Molecular weight: 25.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A068UYY0|A0A068UYY0_COFCA Protein kinase domain-containing protein OS=Coffea canephora OX=49390 GN=GSCOC_T00038627001 PE=3 SV=1
MM1 pKa = 6.86LTKK4 pKa = 10.09RR5 pKa = 11.84TINPFFSAKK14 pKa = 9.96IPSNPNAGRR23 pKa = 11.84HH24 pKa = 5.15GRR26 pKa = 11.84LSLKK30 pKa = 10.36FSPRR34 pKa = 11.84THH36 pKa = 6.58HH37 pKa = 6.75PSSLTTPGKK46 pKa = 9.85IGRR49 pKa = 11.84QMQVRR54 pKa = 11.84LEE56 pKa = 3.96AMALSTFRR64 pKa = 11.84PTFHH68 pKa = 6.92QSPPLPTSISISRR81 pKa = 11.84TPSLFLFITRR91 pKa = 11.84NFRR94 pKa = 11.84HH95 pKa = 6.13HH96 pKa = 5.84QWQQQ100 pKa = 2.52

Molecular weight: 11.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25401

0

25401

10225770

14

5476

402.6

44.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.75 ± 0.014

1.942 ± 0.008

5.205 ± 0.01

6.299 ± 0.016

4.361 ± 0.011

6.525 ± 0.015

2.383 ± 0.006

5.484 ± 0.011

5.994 ± 0.012

10.359 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.358 ± 0.006

4.437 ± 0.01

4.828 ± 0.014

3.676 ± 0.011

5.22 ± 0.012

8.854 ± 0.016

4.747 ± 0.008

6.406 ± 0.011

1.32 ± 0.005

2.853 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski