Canis familiaris papillomavirus 4
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A9XNH4|A9XNH4_9PAPI Replication protein E1 OS=Canis familiaris papillomavirus 4 OX=464980 GN=E1 PE=3 SV=1
MM1 pKa = 7.39 IGKK4 pKa = 9.23 DD5 pKa = 3.29 ATLKK9 pKa = 10.97 DD10 pKa = 3.57 IVLEE14 pKa = 4.12 EE15 pKa = 4.25 QPTPVDD21 pKa = 5.14 DD22 pKa = 4.56 LWCDD26 pKa = 3.55 EE27 pKa = 4.49 EE28 pKa = 4.82 LTLSDD33 pKa = 4.35 EE34 pKa = 4.52 EE35 pKa = 4.79 EE36 pKa = 4.36 EE37 pKa = 4.32 PALNPNFKK45 pKa = 10.04 PFRR48 pKa = 11.84 IQTSCGTCDD57 pKa = 2.79 RR58 pKa = 11.84 GIRR61 pKa = 11.84 IVVLCTTEE69 pKa = 5.02 GIHH72 pKa = 7.2 ALEE75 pKa = 4.3 TLLCRR80 pKa = 11.84 NTSLCCPNCAATYY93 pKa = 9.69 RR94 pKa = 11.84 FEE96 pKa = 5.84 HH97 pKa = 6.64 GGG99 pKa = 3.37
Molecular weight: 11.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.1
IPC2_protein 4.418
IPC_protein 4.304
Toseland 4.151
ProMoST 4.418
Dawson 4.253
Bjellqvist 4.406
Wikipedia 4.126
Rodwell 4.151
Grimsley 4.062
Solomon 4.24
Lehninger 4.202
Nozaki 4.368
DTASelect 4.495
Thurlkill 4.164
EMBOSS 4.139
Sillero 4.418
Patrickios 1.977
IPC_peptide 4.253
IPC2_peptide 4.406
IPC2.peptide.svr19 4.331
Protein with the highest isoelectric point:
>tr|A9XNH3|A9XNH3_9PAPI Protein E7 OS=Canis familiaris papillomavirus 4 OX=464980 GN=E7 PE=3 SV=1
MM1 pKa = 7.36 ARR3 pKa = 11.84 PWSVAGLCAQTGTSFEE19 pKa = 4.61 NIRR22 pKa = 11.84 LPCVFCKK29 pKa = 10.09 QWMDD33 pKa = 3.97 RR34 pKa = 11.84 DD35 pKa = 3.5 DD36 pKa = 4.29 CAAFDD41 pKa = 4.45 FKK43 pKa = 10.79 ILQLSWKK50 pKa = 9.21 GGRR53 pKa = 11.84 PHH55 pKa = 7.3 GCCTACARR63 pKa = 11.84 SIAQRR68 pKa = 11.84 EE69 pKa = 4.25 TARR72 pKa = 11.84 FTSEE76 pKa = 4.24 VITHH80 pKa = 6.88 KK81 pKa = 11.07 DD82 pKa = 3.04 FVDD85 pKa = 3.47 RR86 pKa = 11.84 VGFGLWFIPVRR97 pKa = 11.84 CPICLSLVSAIQKK110 pKa = 9.55 LAAITRR116 pKa = 11.84 KK117 pKa = 9.64 QKK119 pKa = 9.6 FVKK122 pKa = 10.28 VRR124 pKa = 11.84 GRR126 pKa = 11.84 WRR128 pKa = 11.84 TLCTLCTEE136 pKa = 4.97 SDD138 pKa = 3.64 NDD140 pKa = 3.24 WEE142 pKa = 4.13 RR143 pKa = 11.84 RR144 pKa = 11.84 HH145 pKa = 6.15 FEE147 pKa = 3.89 RR148 pKa = 11.84 HH149 pKa = 5.59 CPP151 pKa = 3.56
Molecular weight: 17.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.36
IPC2_protein 8.214
IPC_protein 8.068
Toseland 7.6
ProMoST 8.712
Dawson 8.785
Bjellqvist 9.268
Wikipedia 8.682
Rodwell 8.829
Grimsley 7.512
Solomon 8.916
Lehninger 8.946
Nozaki 9.721
DTASelect 8.887
Thurlkill 8.99
EMBOSS 9.004
Sillero 9.355
Patrickios 5.016
IPC_peptide 8.902
IPC2_peptide 8.858
IPC2.peptide.svr19 8.783
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2373
99
629
395.5
43.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.785 ± 0.289
2.191 ± 0.643
6.827 ± 0.227
5.815 ± 0.46
3.835 ± 0.536
8.049 ± 0.941
2.149 ± 0.162
4.046 ± 0.315
4.34 ± 0.757
8.26 ± 0.396
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.601 ± 0.517
3.203 ± 0.573
6.405 ± 0.858
3.835 ± 0.526
7.501 ± 0.571
7.038 ± 0.612
6.953 ± 0.24
6.785 ± 0.889
1.812 ± 0.325
2.571 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here