Acidipila dinghuensis

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Acidobacteriales; Acidobacteriaceae; Acidipila

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3909 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q1SDS9|A0A4Q1SDS9_9BACT NAD(P)-dependent alcohol dehydrogenase OS=Acidipila dinghuensis OX=1560006 GN=ESZ00_11500 PE=3 SV=1
MM1 pKa = 7.6ASSASIVSLLEE12 pKa = 3.96SLGVPILQEE21 pKa = 3.87VADD24 pKa = 4.05AALGQGVLTLSDD36 pKa = 4.01DD37 pKa = 3.87ASGLTVGVTLSSGQSLHH54 pKa = 6.98ADD56 pKa = 3.31DD57 pKa = 4.19VTFMGSQGFSGHH69 pKa = 6.42FYY71 pKa = 11.29VDD73 pKa = 3.55GLDD76 pKa = 4.06ANPLSATLAGGFAIALTAFDD96 pKa = 3.56VTIANGGMAASTIGGALTIPFFTDD120 pKa = 2.88EE121 pKa = 4.31NGNPEE126 pKa = 4.15TLDD129 pKa = 3.44VEE131 pKa = 5.0VSTKK135 pKa = 10.15QDD137 pKa = 2.76GSLCITLAATTSSPTTPDD155 pKa = 2.92GLVQLVYY162 pKa = 10.8NLPGGLGSVEE172 pKa = 3.83IDD174 pKa = 3.43VASIEE179 pKa = 4.18FDD181 pKa = 3.17EE182 pKa = 5.64SAGTWTIIISGNLIITVPGLNWPSIEE208 pKa = 4.44LRR210 pKa = 11.84GLGIDD215 pKa = 3.45SKK217 pKa = 11.9GNISLQGGWINLPNQMALDD236 pKa = 4.39FYY238 pKa = 10.88GFSIALQALGFGSDD252 pKa = 5.24ASGKK256 pKa = 9.18WIGFNGDD263 pKa = 2.92INLVEE268 pKa = 4.34GLSLGGSVQGLKK280 pKa = 10.9VNLTTGSISFNGVGISFEE298 pKa = 4.07IPGVITIDD306 pKa = 3.57GEE308 pKa = 3.88IDD310 pKa = 4.07HH311 pKa = 6.76IHH313 pKa = 6.35VDD315 pKa = 3.6ANTPNDD321 pKa = 3.87LVPYY325 pKa = 10.1GLMPSIFNSIAPVGPTGPKK344 pKa = 10.34SIDD347 pKa = 3.3LFAGSVKK354 pKa = 10.54VQVLPDD360 pKa = 3.64EE361 pKa = 4.71MDD363 pKa = 3.95LEE365 pKa = 4.28VDD367 pKa = 3.41ANFIVGNFGGQSVFFLALDD386 pKa = 3.49VEE388 pKa = 4.84LPVGIPIFMDD398 pKa = 3.51VSLYY402 pKa = 10.87GLSGLVATGLDD413 pKa = 4.23PDD415 pKa = 4.87PEE417 pKa = 5.62PNDD420 pKa = 4.23TWWQWYY426 pKa = 9.19KK427 pKa = 11.2YY428 pKa = 9.17PAGTSGPQLNATPDD442 pKa = 3.42YY443 pKa = 10.88SATDD447 pKa = 3.67FTKK450 pKa = 10.28WLVPQQGAFAIGGGCTIGTSADD472 pKa = 3.36DD473 pKa = 4.91GYY475 pKa = 9.38TVSAAIMLVILMPGPVISLIGKK497 pKa = 9.87ANILSKK503 pKa = 10.47RR504 pKa = 11.84ISGADD509 pKa = 2.97QDD511 pKa = 4.92ANFEE515 pKa = 4.29AMATYY520 pKa = 10.38DD521 pKa = 3.98GNSGTFDD528 pKa = 3.45LTIDD532 pKa = 3.58AQYY535 pKa = 10.59SIPVVLDD542 pKa = 3.43IEE544 pKa = 4.56ATAEE548 pKa = 4.21LYY550 pKa = 10.96VDD552 pKa = 4.06ATAGEE557 pKa = 4.48WFFAMGKK564 pKa = 9.0PPHH567 pKa = 5.33TQRR570 pKa = 11.84VSARR574 pKa = 11.84IFDD577 pKa = 3.72IFEE580 pKa = 3.78TDD582 pKa = 2.71AYY584 pKa = 10.55FVVSNAGLITGTWTGYY600 pKa = 8.9QGSWSFGPLSASLDD614 pKa = 3.57AYY616 pKa = 10.85LATLAAIQWSPLQIAGGIEE635 pKa = 3.93LHH637 pKa = 7.09GSVQLSAFGIGVGISADD654 pKa = 3.59ALLEE658 pKa = 4.38GCAPNPFWVHH668 pKa = 5.99GEE670 pKa = 3.94LSVEE674 pKa = 5.31LDD676 pKa = 4.43LPWPLPNVGGTISLSWGGDD695 pKa = 3.47DD696 pKa = 5.37GSIPPVPLALSHH708 pKa = 7.03IDD710 pKa = 3.75AALSDD715 pKa = 4.33HH716 pKa = 6.84GDD718 pKa = 3.73SAGKK722 pKa = 8.64PASDD726 pKa = 3.55HH727 pKa = 5.97YY728 pKa = 11.69VLLAHH733 pKa = 7.43RR734 pKa = 11.84SNGPFPDD741 pKa = 3.62LTVQYY746 pKa = 10.21DD747 pKa = 3.49NPQAPGILDD756 pKa = 3.54LTGTALTNWQNRR768 pKa = 11.84VSSGNLADD776 pKa = 4.8MLPDD780 pKa = 3.45MTPDD784 pKa = 3.18NTSRR788 pKa = 11.84TQMAPVLPQDD798 pKa = 3.26THH800 pKa = 6.18FVLNFSHH807 pKa = 6.96PVVDD811 pKa = 3.89MSGGFNNALPQAQLPPEE828 pKa = 4.6VVTTPPPPPPTEE840 pKa = 3.87VGKK843 pKa = 10.61DD844 pKa = 3.29DD845 pKa = 4.09MSNINPNPPSVQFLIRR861 pKa = 11.84HH862 pKa = 5.09SLIDD866 pKa = 3.36VSLYY870 pKa = 10.42EE871 pKa = 4.18WVGGAWSLVASIPPVPPTEE890 pKa = 4.73ASAQADD896 pKa = 3.78TTYY899 pKa = 11.45LSGVWLAPTSATQIQLKK916 pKa = 8.58VVPWMMVEE924 pKa = 4.27GEE926 pKa = 4.31FWTVSWSSTSTPQNYY941 pKa = 7.4GTSFNNQGLDD951 pKa = 3.59FQTTNTQPAAIGTGNAPGLQQGLAFTNAGGSQQPSVAITFPQPEE995 pKa = 4.44VLSSLTAVVAVDD1007 pKa = 3.68EE1008 pKa = 5.04GEE1010 pKa = 4.42LLYY1013 pKa = 10.75AYY1015 pKa = 8.01NAPTCVGDD1023 pKa = 4.4GVTLTPTAQSWDD1035 pKa = 3.88SNFMQWTLTFDD1046 pKa = 3.91PEE1048 pKa = 4.06ATPISTLTLSMQNTSGATLVLYY1070 pKa = 10.7ALDD1073 pKa = 3.71YY1074 pKa = 8.63TVPPTAMAILPKK1086 pKa = 10.67APATYY1091 pKa = 10.87ALRR1094 pKa = 11.84VTTKK1098 pKa = 10.26IEE1100 pKa = 3.73AARR1103 pKa = 11.84VNGGTPQYY1111 pKa = 9.35QTAPDD1116 pKa = 4.21GSPIVEE1122 pKa = 4.22FAYY1125 pKa = 10.18FQTACGPGIAEE1136 pKa = 4.61IGASIPNTSGLPSTPTNTTPVPAEE1160 pKa = 3.76AANFPQLAVNCSTMQQPFTSFPLGGAIDD1188 pKa = 5.15DD1189 pKa = 4.16LTTYY1193 pKa = 7.25TQWSWPLNGAVTAYY1207 pKa = 10.33YY1208 pKa = 10.29GYY1210 pKa = 10.68DD1211 pKa = 3.41VNVEE1215 pKa = 4.09FCEE1218 pKa = 4.66SYY1220 pKa = 11.62VNALYY1225 pKa = 10.57VAAGGRR1231 pKa = 11.84GIANSLHH1238 pKa = 5.59FRR1240 pKa = 11.84CVDD1243 pKa = 3.27RR1244 pKa = 11.84NQNHH1248 pKa = 7.23LLLQTLAIHH1257 pKa = 6.1VPSIPQQSALVATALTVPLPSVIQPSDD1284 pKa = 3.37QDD1286 pKa = 3.61GLLTLTGAQLAGLEE1300 pKa = 4.0KK1301 pKa = 10.33RR1302 pKa = 11.84ALVKK1306 pKa = 9.99IDD1308 pKa = 4.82SNLQPDD1314 pKa = 4.39TAASLQNTAITNVSQLTGPGIQQLNPGIIGIILKK1348 pKa = 9.69EE1349 pKa = 4.03RR1350 pKa = 11.84QEE1352 pKa = 3.97QEE1354 pKa = 3.39AAATARR1360 pKa = 11.84QLWFQPLAPQMQYY1373 pKa = 9.79TLDD1376 pKa = 3.63VVAGSWLGATSDD1388 pKa = 4.36FYY1390 pKa = 10.93PAAGAGTGSLEE1401 pKa = 4.48EE1402 pKa = 4.47IFSASDD1408 pKa = 3.96AISLLKK1414 pKa = 10.67DD1415 pKa = 3.29LEE1417 pKa = 4.25NWYY1420 pKa = 8.14TAQAALTTLEE1430 pKa = 4.18RR1431 pKa = 11.84VTFTTSRR1438 pKa = 11.84YY1439 pKa = 8.54ATFSSQMSNAADD1451 pKa = 3.49QLAGVAGTAPLRR1463 pKa = 11.84HH1464 pKa = 6.33YY1465 pKa = 7.76NTSVDD1470 pKa = 3.77VNTWLADD1477 pKa = 3.48AANGYY1482 pKa = 9.14SAFTTAQTIYY1492 pKa = 8.17TQQRR1496 pKa = 11.84ATLAGVVAQFDD1507 pKa = 3.84PRR1509 pKa = 11.84ADD1511 pKa = 3.77DD1512 pKa = 4.39LAPGAPWPDD1521 pKa = 3.57NGNHH1525 pKa = 6.49ALVTARR1531 pKa = 11.84EE1532 pKa = 4.32ATSQAWQALQQTSVPVFDD1550 pKa = 5.05ALIGALGRR1558 pKa = 11.84ADD1560 pKa = 3.44LTSTQKK1566 pKa = 10.51PVAVPDD1572 pKa = 3.7TEE1574 pKa = 4.21MTLFLDD1580 pKa = 3.49PTGFDD1585 pKa = 2.87IRR1587 pKa = 11.84AILLEE1592 pKa = 4.41SPEE1595 pKa = 4.03ALPWQRR1601 pKa = 11.84IWQWIQLTPISPSSLGLKK1619 pKa = 10.12QIDD1622 pKa = 3.78VLWNSDD1628 pKa = 3.21NTRR1631 pKa = 11.84GLILPLGSPRR1641 pKa = 11.84GRR1643 pKa = 11.84YY1644 pKa = 6.25TLSITFQGNLGAEE1657 pKa = 4.43APCITLNGLPVTEE1670 pKa = 4.44AATIGVLSLGAVIHH1684 pKa = 6.43KK1685 pKa = 9.38PRR1687 pKa = 11.84NLL1689 pKa = 3.35

Molecular weight: 177.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q1SJK8|A0A4Q1SJK8_9BACT Uncharacterized protein OS=Acidipila dinghuensis OX=1560006 GN=ESZ00_07015 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.02RR4 pKa = 11.84TFQPNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SKK15 pKa = 9.6VHH17 pKa = 5.91GFRR20 pKa = 11.84SRR22 pKa = 11.84MKK24 pKa = 8.69TKK26 pKa = 10.4SGAAVLSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.64GRR40 pKa = 11.84KK41 pKa = 8.23RR42 pKa = 11.84VSVSASYY49 pKa = 11.04RR50 pKa = 11.84DD51 pKa = 3.38

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3909

0

3909

1493901

29

2828

382.2

41.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.201 ± 0.051

0.811 ± 0.012

5.098 ± 0.031

5.661 ± 0.045

3.854 ± 0.028

8.031 ± 0.039

2.422 ± 0.02

4.873 ± 0.028

3.361 ± 0.034

10.094 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.191 ± 0.017

3.247 ± 0.037

5.344 ± 0.027

3.898 ± 0.029

6.206 ± 0.045

6.378 ± 0.038

5.989 ± 0.046

6.957 ± 0.029

1.463 ± 0.019

2.922 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski