Streptococcus satellite phage Javan338

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZKQ8|A0A4D5ZKQ8_9VIRU Pleiotropic regulator of exopolysaccharide synthesis competence and biofilm formation OS=Streptococcus satellite phage Javan338 OX=2558647 GN=JavanS338_0003 PE=4 SV=1
MM1 pKa = 6.98TLKK4 pKa = 10.78AFSDD8 pKa = 3.56KK9 pKa = 11.33AKK11 pKa = 9.81TFTFTYY17 pKa = 9.87EE18 pKa = 4.22FKK20 pKa = 11.13DD21 pKa = 3.3LDD23 pKa = 3.81TALVAGHH30 pKa = 7.21ALLGYY35 pKa = 7.49MTGTYY40 pKa = 8.01EE41 pKa = 4.13VPSISITHH49 pKa = 6.69KK50 pKa = 11.13DD51 pKa = 2.96KK52 pKa = 10.45GTLVAEE58 pKa = 4.14YY59 pKa = 11.21VEE61 pKa = 4.55DD62 pKa = 3.61KK63 pKa = 11.0KK64 pKa = 11.47LNKK67 pKa = 8.34TFKK70 pKa = 10.41RR71 pKa = 11.84ICDD74 pKa = 3.7SFKK77 pKa = 10.87DD78 pKa = 4.26YY79 pKa = 11.51YY80 pKa = 9.82NQPVDD85 pKa = 4.45DD86 pKa = 4.12EE87 pKa = 4.7AFEE90 pKa = 4.06EE91 pKa = 4.93RR92 pKa = 11.84YY93 pKa = 9.8KK94 pKa = 10.7RR95 pKa = 11.84EE96 pKa = 3.48RR97 pKa = 11.84VLQLKK102 pKa = 9.95EE103 pKa = 4.26SEE105 pKa = 4.41DD106 pKa = 4.95FEE108 pKa = 5.9DD109 pKa = 4.89LLNKK113 pKa = 8.28VTDD116 pKa = 3.96YY117 pKa = 11.19EE118 pKa = 5.2LEE120 pKa = 4.13LLDD123 pKa = 3.9YY124 pKa = 11.08ADD126 pKa = 5.31RR127 pKa = 11.84LLSDD131 pKa = 4.21KK132 pKa = 9.93PIPMDD137 pKa = 3.3SMTAFGTLEE146 pKa = 3.9MLGDD150 pKa = 3.83EE151 pKa = 5.38SISLLQKK158 pKa = 10.52LDD160 pKa = 3.32VEE162 pKa = 4.81GEE164 pKa = 4.33YY165 pKa = 10.49KK166 pKa = 10.72GLAGYY171 pKa = 7.7TEE173 pKa = 4.17HH174 pKa = 7.28LKK176 pKa = 10.96

Molecular weight: 20.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZQB7|A0A4D5ZQB7_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan338 OX=2558647 GN=JavanS338_0001 PE=3 SV=1
MM1 pKa = 7.64FDD3 pKa = 4.48GFPEE7 pKa = 4.09RR8 pKa = 11.84PKK10 pKa = 10.49YY11 pKa = 9.43MKK13 pKa = 9.51RR14 pKa = 11.84DD15 pKa = 3.77RR16 pKa = 11.84YY17 pKa = 9.18HH18 pKa = 5.94KK19 pKa = 10.09HH20 pKa = 4.58YY21 pKa = 11.02KK22 pKa = 10.26KK23 pKa = 10.36FLKK26 pKa = 8.87YY27 pKa = 8.46TKK29 pKa = 10.33KK30 pKa = 10.73GDD32 pKa = 3.79RR33 pKa = 11.84FWLNGSRR40 pKa = 11.84LL41 pKa = 3.57

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

2946

41

388

147.3

17.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.465 ± 0.438

0.543 ± 0.125

5.737 ± 0.494

8.792 ± 0.644

4.175 ± 0.35

4.447 ± 0.366

1.833 ± 0.26

7.298 ± 0.64

9.47 ± 0.56

10.421 ± 0.609

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.682 ± 0.356

5.363 ± 0.535

3.157 ± 0.488

3.938 ± 0.367

5.261 ± 0.411

5.295 ± 0.295

6.382 ± 0.444

4.684 ± 0.365

0.747 ± 0.167

4.311 ± 0.433
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski