Staphylothermus marinus (strain ATCC 43588 / DSM 3639 / JCM 9404 / F1)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Desulfurococcales; Desulfurococcaceae; Staphylothermus; Staphylothermus marinus

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1570 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3DLN8|A3DLN8_STAMF Photosystem I assembly BtpA OS=Staphylothermus marinus (strain ATCC 43588 / DSM 3639 / JCM 9404 / F1) OX=399550 GN=Smar_0437 PE=3 SV=1
MM1 pKa = 7.71RR2 pKa = 11.84KK3 pKa = 9.4YY4 pKa = 10.64VVSTLLIVIFLVLGTSIYY22 pKa = 10.96CNAQSMEE29 pKa = 4.3DD30 pKa = 3.55SVKK33 pKa = 11.08GIDD36 pKa = 3.26IKK38 pKa = 10.76IVYY41 pKa = 9.7DD42 pKa = 4.03PYY44 pKa = 9.96TDD46 pKa = 3.76YY47 pKa = 11.85GIVTYY52 pKa = 10.32EE53 pKa = 3.8ILFSNTMEE61 pKa = 4.0NAMINLPLVADD72 pKa = 4.95NINNIINVTGSEE84 pKa = 4.25GQVFPYY90 pKa = 10.18TYY92 pKa = 9.46IDD94 pKa = 3.93EE95 pKa = 4.53NTSITILFNNTNTLTITYY113 pKa = 7.62EE114 pKa = 3.34ISGFMNEE121 pKa = 4.07VGINAYY127 pKa = 10.41SGILDD132 pKa = 4.45LSPLSGINVSVEE144 pKa = 3.5IVLPDD149 pKa = 3.63IFNVSTDD156 pKa = 3.42PDD158 pKa = 3.59NSYY161 pKa = 11.2VEE163 pKa = 4.23YY164 pKa = 11.02DD165 pKa = 3.29NGLTIIKK172 pKa = 9.85INNPDD177 pKa = 2.64IYY179 pKa = 10.65AIYY182 pKa = 10.07IEE184 pKa = 4.65KK185 pKa = 10.69SLFATTTTATTTTTTATTTSSILSPPITSTTSPTATTTTTAPPPTTTTTTTTTTTTSPSTLSPGGTLGLSPFMIGLIIVVLAVAVLIALLLRR277 pKa = 11.84RR278 pKa = 4.46

Molecular weight: 29.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A3DNH4|RL31_STAMF 50S ribosomal protein L31e OS=Staphylothermus marinus (strain ATCC 43588 / DSM 3639 / JCM 9404 / F1) OX=399550 GN=rpl31e PE=3 SV=1
MM1 pKa = 7.61ARR3 pKa = 11.84NKK5 pKa = 9.96PLARR9 pKa = 11.84KK10 pKa = 9.11LRR12 pKa = 11.84LAAAYY17 pKa = 9.5KK18 pKa = 10.0EE19 pKa = 4.01NRR21 pKa = 11.84PVPIWVSVKK30 pKa = 9.39TRR32 pKa = 11.84LKK34 pKa = 9.19VRR36 pKa = 11.84RR37 pKa = 11.84GFRR40 pKa = 11.84LRR42 pKa = 11.84HH43 pKa = 4.26WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.95LKK50 pKa = 10.03VV51 pKa = 2.95

Molecular weight: 6.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1570

0

1570

454805

36

1701

289.7

32.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.834 ± 0.063

0.629 ± 0.02

4.67 ± 0.041

6.841 ± 0.073

3.577 ± 0.041

6.453 ± 0.056

1.751 ± 0.025

10.406 ± 0.073

7.517 ± 0.075

10.394 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.241 ± 0.024

4.233 ± 0.052

4.363 ± 0.041

1.744 ± 0.027

5.43 ± 0.058

5.658 ± 0.057

4.778 ± 0.066

7.164 ± 0.051

1.172 ± 0.025

5.145 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski