Micromonospora haikouensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5591 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D0WS75|A0A0D0WS75_9ACTN Probable RNA 2'-phosphotransferase OS=Micromonospora haikouensis OX=686309 GN=kptA PE=3 SV=1
MM1 pKa = 7.66SEE3 pKa = 4.02VATDD7 pKa = 3.8QLQVWVDD14 pKa = 3.37QDD16 pKa = 3.81LCTGDD21 pKa = 3.99GLCVQYY27 pKa = 10.98APEE30 pKa = 4.07VFEE33 pKa = 4.79FDD35 pKa = 3.39VDD37 pKa = 3.3GLAYY41 pKa = 10.67VKK43 pKa = 10.76GADD46 pKa = 4.81GEE48 pKa = 4.43LQLAPGSRR56 pKa = 11.84VGVPEE61 pKa = 3.85HH62 pKa = 6.58LRR64 pKa = 11.84LEE66 pKa = 4.75VIDD69 pKa = 4.27SAKK72 pKa = 10.05EE73 pKa = 3.91CPGEE77 pKa = 4.37CIHH80 pKa = 6.22VVRR83 pKa = 11.84GDD85 pKa = 3.65GVEE88 pKa = 4.14VAGPDD93 pKa = 4.19AEE95 pKa = 5.6DD96 pKa = 3.86DD97 pKa = 3.66

Molecular weight: 10.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D0VJY3|A0A0D0VJY3_9ACTN 3-oxoacyl-ACP reductase OS=Micromonospora haikouensis OX=686309 GN=GA0070558_11089 PE=4 SV=1
MM1 pKa = 7.92AEE3 pKa = 3.99AQKK6 pKa = 10.24ATTRR10 pKa = 11.84PAAQRR15 pKa = 11.84TTAKK19 pKa = 8.77KK20 pKa = 7.48TAAAGRR26 pKa = 11.84ATGTTRR32 pKa = 11.84AATTATRR39 pKa = 11.84RR40 pKa = 11.84STVTKK45 pKa = 10.39ASPNASGAGAGRR57 pKa = 11.84VPAKK61 pKa = 10.35KK62 pKa = 10.12AVAKK66 pKa = 10.15KK67 pKa = 10.06AAATKK72 pKa = 10.0VATSRR77 pKa = 11.84TPVGSRR83 pKa = 11.84TAAKK87 pKa = 9.77KK88 pKa = 10.62APATRR93 pKa = 11.84ASAKK97 pKa = 9.68KK98 pKa = 10.29APAAKK103 pKa = 7.12TTTARR108 pKa = 11.84KK109 pKa = 4.74TTAAKK114 pKa = 6.51TTAAKK119 pKa = 7.22TTATKK124 pKa = 9.09TARR127 pKa = 11.84SATGAAATKK136 pKa = 9.17RR137 pKa = 11.84TTAATKK143 pKa = 10.1KK144 pKa = 10.22ASPAAKK150 pKa = 8.87RR151 pKa = 11.84TTTTAAKK158 pKa = 8.29RR159 pKa = 11.84TTAAKK164 pKa = 10.24RR165 pKa = 11.84AGATAAKK172 pKa = 8.2RR173 pKa = 11.84TTAAAAKK180 pKa = 9.15KK181 pKa = 10.4APTAAKK187 pKa = 8.6RR188 pKa = 11.84TAAAAKK194 pKa = 9.14KK195 pKa = 10.33APAAKK200 pKa = 8.72KK201 pKa = 6.09TTARR205 pKa = 11.84KK206 pKa = 5.48TTVAKK211 pKa = 10.21KK212 pKa = 10.54APAAKK217 pKa = 8.72KK218 pKa = 6.09TTARR222 pKa = 11.84KK223 pKa = 5.2TTSSRR228 pKa = 11.84STATRR233 pKa = 11.84TATAKK238 pKa = 10.18KK239 pKa = 9.95APARR243 pKa = 11.84KK244 pKa = 8.34STSSRR249 pKa = 11.84NPP251 pKa = 3.06

Molecular weight: 24.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5591

0

5591

1931850

29

10006

345.5

36.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.564 ± 0.057

0.747 ± 0.008

6.157 ± 0.031

4.915 ± 0.03

2.661 ± 0.019

9.64 ± 0.032

2.145 ± 0.015

2.886 ± 0.023

1.571 ± 0.024

10.453 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.527 ± 0.013

1.779 ± 0.022

6.459 ± 0.032

2.663 ± 0.019

8.438 ± 0.036

4.504 ± 0.025

6.07 ± 0.034

9.168 ± 0.028

1.599 ± 0.014

2.054 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski