Janthinobacterium sp. CG23_2
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7002 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S4KGQ6|A0A0S4KGQ6_9BURK Transposase OS=Janthinobacterium sp. CG23_2 OX=1706231 GN=BN3177_12559 PE=4 SV=1
MM1 pKa = 7.59 VVIADD6 pKa = 3.8 AAVSPEE12 pKa = 3.85 WQSQVSDD19 pKa = 4.14 WIVKK23 pKa = 9.3 SGCRR27 pKa = 11.84 YY28 pKa = 8.2 MIAWGVDD35 pKa = 3.1 CSSWDD40 pKa = 3.85 DD41 pKa = 3.77 AVDD44 pKa = 3.52 MANIEE49 pKa = 4.18 AFDD52 pKa = 4.43 FGDD55 pKa = 3.94 IPDD58 pKa = 3.39 QHH60 pKa = 7.22 FIPTAWHH67 pKa = 6.67 ADD69 pKa = 3.63 DD70 pKa = 5.82 PIEE73 pKa = 5.28 DD74 pKa = 3.42 VFGFCKK80 pKa = 9.97 HH81 pKa = 6.48 DD82 pKa = 3.72 AAHH85 pKa = 6.56 PVVEE89 pKa = 4.81 LKK91 pKa = 9.11 QTVLLHH97 pKa = 6.3 IADD100 pKa = 4.57 AADD103 pKa = 3.85 EE104 pKa = 4.55 DD105 pKa = 4.67 ALLQAYY111 pKa = 8.58 AAAA114 pKa = 5.03
Molecular weight: 12.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.872
IPC_protein 3.859
Toseland 3.643
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.846
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.821
Nozaki 3.999
DTASelect 4.279
Thurlkill 3.706
EMBOSS 3.846
Sillero 3.999
Patrickios 1.926
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.879
Protein with the highest isoelectric point:
>tr|A0A0S4P0H8|A0A0S4P0H8_9BURK Dihydrofolate synthase/folylpolyglutamate synthase OS=Janthinobacterium sp. CG23_2 OX=1706231 GN=BN3177_4317 PE=3 SV=1
MM1 pKa = 6.98 QKK3 pKa = 9.94 NRR5 pKa = 11.84 AARR8 pKa = 11.84 ARR10 pKa = 11.84 LVVAFARR17 pKa = 11.84 SGAHH21 pKa = 4.56 RR22 pKa = 11.84 QPRR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 APGPGITWRR36 pKa = 11.84 LL37 pKa = 3.3
Molecular weight: 4.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.504
IPC2_protein 11.125
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.457
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.193
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.167
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7002
0
7002
2239087
37
10633
319.8
34.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.971 ± 0.049
0.943 ± 0.012
5.596 ± 0.026
4.903 ± 0.03
3.531 ± 0.02
8.263 ± 0.038
2.209 ± 0.016
4.626 ± 0.027
3.623 ± 0.028
10.203 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.604 ± 0.015
3.109 ± 0.032
4.979 ± 0.038
3.818 ± 0.021
6.514 ± 0.032
5.744 ± 0.03
5.352 ± 0.045
7.137 ± 0.023
1.321 ± 0.014
2.555 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here