Mangshi virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Seadornavirus; unclassified Seadornavirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S3JNF8|A0A0S3JNF8_9REOV VP12 OS=Mangshi virus OX=1766829 PE=4 SV=1
MM1 pKa = 7.4SRR3 pKa = 11.84NRR5 pKa = 11.84QIKK8 pKa = 9.23LRR10 pKa = 11.84NGQGTIIDD18 pKa = 3.75RR19 pKa = 11.84NGKK22 pKa = 9.86FEE24 pKa = 4.21VLVRR28 pKa = 11.84RR29 pKa = 11.84LDD31 pKa = 3.47IDD33 pKa = 3.67HH34 pKa = 7.04NLPLASTVSSGSRR47 pKa = 11.84VDD49 pKa = 3.15IVKK52 pKa = 10.23FLVDD56 pKa = 4.24FVGVTYY62 pKa = 10.13IDD64 pKa = 3.35SGGSVDD70 pKa = 4.42FSEE73 pKa = 6.21SEE75 pKa = 4.06LEE77 pKa = 4.18HH78 pKa = 6.35YY79 pKa = 9.95KK80 pKa = 11.15GNSTLLVASTAFGPLFIKK98 pKa = 10.44KK99 pKa = 9.15IRR101 pKa = 11.84QIPICNCAFIDD112 pKa = 3.59NKK114 pKa = 10.9YY115 pKa = 10.78NILAACRR122 pKa = 11.84GVLKK126 pKa = 9.5LTNDD130 pKa = 3.49RR131 pKa = 11.84NGYY134 pKa = 8.24RR135 pKa = 11.84YY136 pKa = 10.8GMILEE141 pKa = 4.63RR142 pKa = 11.84CHH144 pKa = 6.09PPKK147 pKa = 10.56INFTNLVEE155 pKa = 4.36AVVNLAYY162 pKa = 9.48MHH164 pKa = 6.74KK165 pKa = 9.86SEE167 pKa = 4.0PHH169 pKa = 6.09ALHH172 pKa = 7.09GDD174 pKa = 3.81VNPSNIMSDD183 pKa = 3.31QQGVLKK189 pKa = 10.74LVDD192 pKa = 3.84PVCILEE198 pKa = 4.22GQVNIANVDD207 pKa = 3.81YY208 pKa = 11.5EE209 pKa = 4.32EE210 pKa = 4.51LTQQEE215 pKa = 4.39EE216 pKa = 4.03MRR218 pKa = 11.84LFILSLLQILGSQFKK233 pKa = 10.73VRR235 pKa = 11.84IDD237 pKa = 3.43KK238 pKa = 11.18VKK240 pKa = 10.24IDD242 pKa = 3.7YY243 pKa = 9.71SKK245 pKa = 10.72CQPEE249 pKa = 4.2FTISDD254 pKa = 3.76DD255 pKa = 3.75SSNPSLADD263 pKa = 3.14VLSFNVSDD271 pKa = 4.54AIEE274 pKa = 4.13WKK276 pKa = 10.65DD277 pKa = 3.54RR278 pKa = 11.84MLSPRR283 pKa = 11.84AIPEE287 pKa = 3.44PSFRR291 pKa = 11.84HH292 pKa = 6.67DD293 pKa = 3.65YY294 pKa = 11.25YY295 pKa = 11.61KK296 pKa = 10.21LTEE299 pKa = 4.32LNEE302 pKa = 4.2QVSDD306 pKa = 5.06LDD308 pKa = 5.05DD309 pKa = 5.92DD310 pKa = 5.62DD311 pKa = 6.65DD312 pKa = 3.76II313 pKa = 6.96

Molecular weight: 35.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S3JNE2|A0A0S3JNE2_9REOV VP11 OS=Mangshi virus OX=1766829 PE=4 SV=1
MM1 pKa = 7.94DD2 pKa = 4.01VLSKK6 pKa = 10.91KK7 pKa = 8.32SLRR10 pKa = 11.84EE11 pKa = 3.54FLAFLQEE18 pKa = 4.43TPFEE22 pKa = 4.4NALSMTDD29 pKa = 3.02GTAPHH34 pKa = 6.74IPVNVVVQEE43 pKa = 4.1YY44 pKa = 10.83NRR46 pKa = 11.84DD47 pKa = 3.47PYY49 pKa = 10.89IEE51 pKa = 4.44GTTLLDD57 pKa = 3.75GAPRR61 pKa = 11.84TSVRR65 pKa = 11.84NVTGMIRR72 pKa = 11.84SILSYY77 pKa = 7.56VTCGSGNYY85 pKa = 10.03VGMVDD90 pKa = 3.28VYY92 pKa = 10.83TRR94 pKa = 11.84TRR96 pKa = 11.84SIFDD100 pKa = 3.25ILNSRR105 pKa = 11.84FVNVNTAGLNYY116 pKa = 9.45VRR118 pKa = 11.84LVGAMTVNKK127 pKa = 9.83MSVEE131 pKa = 3.67QRR133 pKa = 11.84KK134 pKa = 8.62RR135 pKa = 11.84VIICIKK141 pKa = 10.58LIAEE145 pKa = 4.48KK146 pKa = 10.92DD147 pKa = 3.94NNLDD151 pKa = 3.41AYY153 pKa = 11.67NNFVNNDD160 pKa = 3.28PFLRR164 pKa = 11.84ICEE167 pKa = 4.27VKK169 pKa = 10.79FNVHH173 pKa = 4.96DD174 pKa = 4.2HH175 pKa = 6.45KK176 pKa = 11.39NITSVPDD183 pKa = 3.52NLSVLGLAGLNKK195 pKa = 9.47YY196 pKa = 10.59LKK198 pKa = 9.85IEE200 pKa = 3.93RR201 pKa = 11.84LMVHH205 pKa = 6.07NGVRR209 pKa = 11.84SVNRR213 pKa = 11.84EE214 pKa = 3.66YY215 pKa = 11.18EE216 pKa = 4.08EE217 pKa = 4.24YY218 pKa = 8.83KK219 pKa = 9.96TLRR222 pKa = 11.84NGSAFRR228 pKa = 11.84LQITDD233 pKa = 3.43LQDD236 pKa = 3.27AYY238 pKa = 11.28RR239 pKa = 11.84DD240 pKa = 3.77ALNPAGGGRR249 pKa = 11.84PPIIVV254 pKa = 3.26

Molecular weight: 28.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

5983

199

1214

498.6

55.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.521 ± 0.642

1.086 ± 0.125

6.351 ± 0.282

5.081 ± 0.246

3.761 ± 0.226

5.833 ± 0.242

2.022 ± 0.208

6.251 ± 0.221

5.382 ± 0.496

8.524 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.226 ± 0.225

5.649 ± 0.39

4.112 ± 0.357

3.777 ± 0.416

5.666 ± 0.242

7.12 ± 0.288

6.569 ± 0.495

7.672 ± 0.403

0.635 ± 0.143

3.727 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski