Andreesenia angusta

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Gottschalkiaceae; Andreesenia

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2379 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S1V8V8|A0A1S1V8V8_9FIRM Uncharacterized protein OS=Andreesenia angusta OX=39480 GN=EUAN_08350 PE=4 SV=1
MM1 pKa = 7.86DD2 pKa = 4.96GEE4 pKa = 4.43VNKK7 pKa = 9.83IRR9 pKa = 11.84KK10 pKa = 8.27EE11 pKa = 4.07WMSVQYY17 pKa = 10.52LYY19 pKa = 11.34EE20 pKa = 4.04KK21 pKa = 10.32VAYY24 pKa = 10.23LRR26 pKa = 11.84GLAEE30 pKa = 4.25GLEE33 pKa = 4.39IDD35 pKa = 5.47DD36 pKa = 3.92STKK39 pKa = 10.54EE40 pKa = 4.04GKK42 pKa = 9.41LLKK45 pKa = 10.93SMIVVMEE52 pKa = 4.48DD53 pKa = 2.75MVDD56 pKa = 4.26AINEE60 pKa = 3.95NTEE63 pKa = 4.11EE64 pKa = 4.11IEE66 pKa = 4.4EE67 pKa = 3.99ISSYY71 pKa = 11.11VEE73 pKa = 5.09IIDD76 pKa = 3.96EE77 pKa = 4.73DD78 pKa = 4.09LSEE81 pKa = 4.57MEE83 pKa = 5.45DD84 pKa = 3.02IVYY87 pKa = 10.77DD88 pKa = 4.06EE89 pKa = 6.3DD90 pKa = 5.88DD91 pKa = 5.81DD92 pKa = 5.32EE93 pKa = 7.24DD94 pKa = 5.68DD95 pKa = 4.81YY96 pKa = 12.15YY97 pKa = 11.61DD98 pKa = 5.71DD99 pKa = 5.24EE100 pKa = 5.28DD101 pKa = 3.98FEE103 pKa = 5.16YY104 pKa = 10.61IEE106 pKa = 4.99LVCPSCGEE114 pKa = 4.15EE115 pKa = 3.57ISVDD119 pKa = 3.68RR120 pKa = 11.84EE121 pKa = 4.17LIDD124 pKa = 4.74DD125 pKa = 4.66GEE127 pKa = 4.62VFCPNCEE134 pKa = 3.98EE135 pKa = 4.7KK136 pKa = 10.55IEE138 pKa = 3.96II139 pKa = 4.46

Molecular weight: 16.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S1V425|A0A1S1V425_9FIRM Peripla_BP_6 domain-containing protein OS=Andreesenia angusta OX=39480 GN=EUAN_22950 PE=4 SV=1
MM1 pKa = 7.57LCGFLRR7 pKa = 11.84PTACWCLEE15 pKa = 3.89RR16 pKa = 11.84SHH18 pKa = 6.94RR19 pKa = 11.84PLPRR23 pKa = 11.84PRR25 pKa = 11.84SLAVAGEE32 pKa = 4.2AACATRR38 pKa = 11.84ISRR41 pKa = 11.84VRR43 pKa = 11.84QCLLAASWGLFQSPFSNKK61 pKa = 9.06TGLLCHH67 pKa = 6.29SAEE70 pKa = 4.17PSDD73 pKa = 4.47PLRR76 pKa = 11.84CGNAPPQKK84 pKa = 10.52LRR86 pKa = 11.84FVQLAFGHH94 pKa = 5.66MLSVFGSSGAVLRR107 pKa = 11.84TAPAIVSTFYY117 pKa = 10.88TRR119 pKa = 11.84TGLKK123 pKa = 9.14LWRR126 pKa = 11.84SFLHH130 pKa = 6.27FRR132 pKa = 11.84EE133 pKa = 4.42LEE135 pKa = 4.32KK136 pKa = 10.93ARR138 pKa = 11.84HH139 pKa = 5.44PPDD142 pKa = 4.96LNFLYY147 pKa = 9.98MKK149 pKa = 9.75FISDD153 pKa = 4.22IIMTIKK159 pKa = 10.66

Molecular weight: 17.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2379

0

2379

709130

29

1966

298.1

33.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.477 ± 0.053

0.874 ± 0.018

5.772 ± 0.038

8.856 ± 0.058

3.837 ± 0.038

7.321 ± 0.045

1.452 ± 0.022

8.138 ± 0.047

7.783 ± 0.043

8.956 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.854 ± 0.022

4.278 ± 0.034

2.935 ± 0.029

2.329 ± 0.026

4.466 ± 0.039

7.31 ± 0.057

4.645 ± 0.033

7.29 ± 0.042

0.685 ± 0.016

3.74 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski