Paraliobacillus ryukyuensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Paraliobacillus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3097 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A366ECN8|A0A366ECN8_9BACI Uncharacterized protein OS=Paraliobacillus ryukyuensis OX=200904 GN=DES48_104185 PE=4 SV=1
MM1 pKa = 7.47QSQSLQGAQEE11 pKa = 4.07LLCFNAEE18 pKa = 3.99KK19 pKa = 10.81VYY21 pKa = 11.07DD22 pKa = 3.79WVILQNTINQNVPAADD38 pKa = 3.33IGVLPIDD45 pKa = 3.96PVSPAVSNLTTRR57 pKa = 11.84CYY59 pKa = 10.65LVDD62 pKa = 4.26PLTGNPLPPNAEE74 pKa = 3.78IEE76 pKa = 4.51VVEE79 pKa = 4.24TADD82 pKa = 3.54RR83 pKa = 11.84QDD85 pKa = 2.98RR86 pKa = 11.84MFVIDD91 pKa = 4.64GSQVTLQRR99 pKa = 11.84VSFEE103 pKa = 3.79KK104 pKa = 10.71VINVVIEE111 pKa = 3.91FSGFDD116 pKa = 3.11GTTPFVEE123 pKa = 3.86ISDD126 pKa = 4.91PIEE129 pKa = 3.58ITIPEE134 pKa = 4.57SVFLCAPEE142 pKa = 4.13GTRR145 pKa = 11.84LVVRR149 pKa = 11.84ISDD152 pKa = 4.07VEE154 pKa = 4.24CSASVNGTAGEE165 pKa = 4.19LVSVDD170 pKa = 4.04LTLDD174 pKa = 3.92LCQSVQAVADD184 pKa = 3.7VTLEE188 pKa = 3.94LVADD192 pKa = 4.77FCEE195 pKa = 4.48PRR197 pKa = 11.84DD198 pKa = 3.76NLITEE203 pKa = 4.83DD204 pKa = 4.58CPNPIIPPQCPVVFPGITGNNGTPTVTNNN233 pKa = 2.91

Molecular weight: 25.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A366ED15|A0A366ED15_9BACI Acetyltransferase (GNAT) family protein OS=Paraliobacillus ryukyuensis OX=200904 GN=DES48_10255 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNTRR10 pKa = 11.84KK11 pKa = 9.78RR12 pKa = 11.84KK13 pKa = 8.32KK14 pKa = 8.57VHH16 pKa = 5.61GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 10.3NGRR28 pKa = 11.84KK29 pKa = 9.11VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.99KK37 pKa = 10.04GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3097

0

3097

874981

25

1605

282.5

31.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.057 ± 0.04

0.6 ± 0.013

5.454 ± 0.041

7.173 ± 0.058

4.423 ± 0.038

6.419 ± 0.044

2.118 ± 0.023

8.1 ± 0.048

6.564 ± 0.037

9.552 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.737 ± 0.021

4.771 ± 0.033

3.394 ± 0.029

4.59 ± 0.042

3.777 ± 0.031

5.816 ± 0.033

5.902 ± 0.034

6.933 ± 0.035

0.977 ± 0.016

3.644 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski