Arthrobacter phage Kuleana

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Kuleanavirus; Arthrobacter virus Kuleana

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W8L7|A0A5Q2W8L7_9CAUD RusA-like resolvase OS=Arthrobacter phage Kuleana OX=2653270 GN=45 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.49LVTINGNGGNDD13 pKa = 3.4YY14 pKa = 10.98DD15 pKa = 4.62VSVHH19 pKa = 6.7RR20 pKa = 11.84ADD22 pKa = 4.4CADD25 pKa = 3.39VPKK28 pKa = 9.97ATRR31 pKa = 11.84GHH33 pKa = 6.97DD34 pKa = 3.64YY35 pKa = 10.86FEE37 pKa = 5.06EE38 pKa = 4.02EE39 pKa = 4.5HH40 pKa = 5.95EE41 pKa = 4.63SKK43 pKa = 10.63RR44 pKa = 11.84ALWLDD49 pKa = 3.52YY50 pKa = 11.14NADD53 pKa = 3.78FLAEE57 pKa = 4.54GPGSAWPIHH66 pKa = 6.48FYY68 pKa = 10.59PCTAGLPDD76 pKa = 4.09GGTYY80 pKa = 8.78NTDD83 pKa = 3.17TDD85 pKa = 3.57EE86 pKa = 4.64GKK88 pKa = 10.61

Molecular weight: 9.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WE41|A0A5Q2WE41_9CAUD Uncharacterized protein OS=Arthrobacter phage Kuleana OX=2653270 GN=33 PE=4 SV=1
MM1 pKa = 7.52GRR3 pKa = 11.84PPLPVGTWGAIRR15 pKa = 11.84VEE17 pKa = 4.82RR18 pKa = 11.84IAGGYY23 pKa = 7.67RR24 pKa = 11.84ARR26 pKa = 11.84ARR28 pKa = 11.84FRR30 pKa = 11.84DD31 pKa = 3.49FDD33 pKa = 3.39GRR35 pKa = 11.84TRR37 pKa = 11.84DD38 pKa = 3.38VEE40 pKa = 4.21RR41 pKa = 11.84SGKK44 pKa = 8.31TKK46 pKa = 10.24GAARR50 pKa = 11.84AALTADD56 pKa = 3.52LTEE59 pKa = 4.3RR60 pKa = 11.84TAPAGDD66 pKa = 4.84EE67 pKa = 4.08ITASTRR73 pKa = 11.84LQLVAEE79 pKa = 4.83IWRR82 pKa = 11.84TEE84 pKa = 3.57KK85 pKa = 10.55WPDD88 pKa = 3.01LAEE91 pKa = 4.31NSRR94 pKa = 11.84KK95 pKa = 9.83RR96 pKa = 11.84YY97 pKa = 9.47RR98 pKa = 11.84DD99 pKa = 3.27ALEE102 pKa = 4.26DD103 pKa = 4.42HH104 pKa = 6.97ILPGLGALTVSEE116 pKa = 4.59CSVTRR121 pKa = 11.84IDD123 pKa = 4.51RR124 pKa = 11.84FLKK127 pKa = 9.48ATAASTGAPSAKK139 pKa = 9.33VCRR142 pKa = 11.84SVLSGILGLAVRR154 pKa = 11.84HH155 pKa = 5.79GAAATNPVRR164 pKa = 11.84DD165 pKa = 3.64VAGITVTPKK174 pKa = 10.38EE175 pKa = 4.0IRR177 pKa = 11.84ALTLEE182 pKa = 4.66EE183 pKa = 3.61IRR185 pKa = 11.84AARR188 pKa = 11.84AAVRR192 pKa = 11.84SWQLGEE198 pKa = 4.0PLAEE202 pKa = 4.04GRR204 pKa = 11.84PRR206 pKa = 11.84RR207 pKa = 11.84GRR209 pKa = 11.84PPTQDD214 pKa = 3.83LLDD217 pKa = 4.06ILDD220 pKa = 4.63LLLATGARR228 pKa = 11.84IGEE231 pKa = 4.28LLAVQWSDD239 pKa = 3.16VDD241 pKa = 5.71LEE243 pKa = 4.3AQTLTISGTIISTEE257 pKa = 4.08EE258 pKa = 3.79KK259 pKa = 9.53PSRR262 pKa = 11.84LVRR265 pKa = 11.84QAHH268 pKa = 6.22PKK270 pKa = 9.42SSSSRR275 pKa = 11.84RR276 pKa = 11.84RR277 pKa = 11.84LTLPPFAIDD286 pKa = 2.95ALMRR290 pKa = 11.84RR291 pKa = 11.84RR292 pKa = 11.84LAVTVANVHH301 pKa = 6.45DD302 pKa = 4.25VVFPSTEE309 pKa = 3.7GTLRR313 pKa = 11.84DD314 pKa = 3.73PGSVRR319 pKa = 11.84KK320 pKa = 9.05QLGKK324 pKa = 10.57VLGPAGLGWVTPHH337 pKa = 6.16VFRR340 pKa = 11.84KK341 pKa = 7.9TVATVLDD348 pKa = 3.78RR349 pKa = 11.84AEE351 pKa = 4.51DD352 pKa = 3.53LRR354 pKa = 11.84TASDD358 pKa = 3.67QLGHH362 pKa = 7.23AGTDD366 pKa = 3.2VTRR369 pKa = 11.84RR370 pKa = 11.84HH371 pKa = 4.93YY372 pKa = 10.54VEE374 pKa = 4.53RR375 pKa = 11.84THH377 pKa = 6.64EE378 pKa = 4.5GPDD381 pKa = 3.15VRR383 pKa = 11.84EE384 pKa = 3.8ALEE387 pKa = 3.88RR388 pKa = 11.84LVRR391 pKa = 11.84GSS393 pKa = 3.1

Molecular weight: 42.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

12284

26

787

161.6

17.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.61 ± 0.385

0.944 ± 0.121

5.698 ± 0.194

6.472 ± 0.35

2.613 ± 0.225

9.964 ± 0.535

1.53 ± 0.144

3.623 ± 0.206

3.598 ± 0.26

9.02 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.043 ± 0.114

2.369 ± 0.182

5.536 ± 0.319

2.703 ± 0.172

7.766 ± 0.413

5.332 ± 0.301

5.528 ± 0.334

8.344 ± 0.368

2.361 ± 0.156

1.946 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski