Dyella thiooxydans
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3854 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A160N617|A0A160N617_9GAMM Aminomethyltransferase OS=Dyella thiooxydans OX=445710 GN=gcvT PE=3 SV=1
MM1 pKa = 7.61 TDD3 pKa = 2.73 STAPASRR10 pKa = 11.84 KK11 pKa = 7.81 WMCVVCGFIYY21 pKa = 10.64 DD22 pKa = 3.74 EE23 pKa = 4.37 ALGVPEE29 pKa = 4.86 EE30 pKa = 5.24 DD31 pKa = 3.21 IAPGTRR37 pKa = 11.84 WEE39 pKa = 4.7 DD40 pKa = 3.78 VPDD43 pKa = 3.14 TWTCPDD49 pKa = 3.84 CGATKK54 pKa = 10.7 DD55 pKa = 3.9 DD56 pKa = 4.11 FEE58 pKa = 4.95 MIEE61 pKa = 3.83 VDD63 pKa = 3.41
Molecular weight: 7.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.706
IPC_protein 3.63
Toseland 3.427
ProMoST 3.745
Dawson 3.643
Bjellqvist 3.872
Wikipedia 3.605
Rodwell 3.478
Grimsley 3.35
Solomon 3.605
Lehninger 3.567
Nozaki 3.77
DTASelect 3.973
Thurlkill 3.503
EMBOSS 3.605
Sillero 3.757
Patrickios 1.85
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.773
Protein with the highest isoelectric point:
>tr|A0A160N520|A0A160N520_9GAMM Uncharacterized protein OS=Dyella thiooxydans OX=445710 GN=ATSB10_35830 PE=4 SV=1
MM1 pKa = 7.6 LSASLRR7 pKa = 11.84 SHH9 pKa = 6.5 LPVRR13 pKa = 11.84 RR14 pKa = 11.84 FTAHH18 pKa = 6.42 PRR20 pKa = 11.84 NPRR23 pKa = 11.84 HH24 pKa = 6.03 ALQRR28 pKa = 11.84 MPRR31 pKa = 11.84 TSRR34 pKa = 11.84 PRR36 pKa = 11.84 AAASRR41 pKa = 3.78
Molecular weight: 4.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.513
IPC2_protein 11.184
IPC_protein 12.793
Toseland 12.954
ProMoST 13.466
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.457
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.193
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.204
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3854
0
3854
1249098
37
3267
324.1
35.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.986 ± 0.055
0.826 ± 0.012
5.855 ± 0.027
5.021 ± 0.041
3.347 ± 0.022
8.638 ± 0.05
2.555 ± 0.02
3.986 ± 0.026
2.659 ± 0.034
11.001 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.287 ± 0.02
2.326 ± 0.032
5.522 ± 0.035
3.7 ± 0.027
7.666 ± 0.052
5.177 ± 0.039
4.989 ± 0.041
7.518 ± 0.038
1.547 ± 0.017
2.395 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here