Pyrrhula pyrrhula polyomavirus 1
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I6RKB0|A0A2I6RKB0_9POLY Capsid protein VP1 OS=Pyrrhula pyrrhula polyomavirus 1 OX=1891751 PE=3 SV=1
MM1 pKa = 7.27 STLQKK6 pKa = 10.89 LIDD9 pKa = 4.37 LLGLPPSATEE19 pKa = 3.5 ADD21 pKa = 3.51 VRR23 pKa = 11.84 SAYY26 pKa = 9.89 RR27 pKa = 11.84 KK28 pKa = 9.5 KK29 pKa = 10.66 ALEE32 pKa = 3.77 FHH34 pKa = 7.12 PDD36 pKa = 3.04 KK37 pKa = 11.5 GGDD40 pKa = 3.6 PEE42 pKa = 5.25 RR43 pKa = 11.84 MKK45 pKa = 10.73 EE46 pKa = 3.98 LNRR49 pKa = 11.84 LMDD52 pKa = 4.19 EE53 pKa = 4.43 FRR55 pKa = 11.84 HH56 pKa = 5.32 SQSLFCDD63 pKa = 3.38 EE64 pKa = 4.89 TLDD67 pKa = 4.59 SDD69 pKa = 5.11 SDD71 pKa = 4.4 DD72 pKa = 4.11 GDD74 pKa = 3.9 SPGPSQRR81 pKa = 11.84 TSTPEE86 pKa = 3.81 PGTGKK91 pKa = 10.39 DD92 pKa = 3.25 SGHH95 pKa = 5.77 GTFEE99 pKa = 4.29 PEE101 pKa = 3.84 VNSWHH106 pKa = 7.14 AIDD109 pKa = 4.97 YY110 pKa = 10.56 DD111 pKa = 3.64 RR112 pKa = 11.84 AYY114 pKa = 10.53 CRR116 pKa = 11.84 LMEE119 pKa = 4.96 LKK121 pKa = 9.57 WCLEE125 pKa = 4.02 SFFTSTEE132 pKa = 3.57 RR133 pKa = 11.84 RR134 pKa = 11.84 KK135 pKa = 10.21 QGLTPEE141 pKa = 3.97 YY142 pKa = 10.67 LEE144 pKa = 4.28 LKK146 pKa = 10.41 RR147 pKa = 11.84 RR148 pKa = 11.84 FQAVPWRR155 pKa = 11.84 VFDD158 pKa = 4.01 NVFNMDD164 pKa = 4.08 YY165 pKa = 10.73 II166 pKa = 4.95
Molecular weight: 19.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.981
IPC2_protein 5.003
IPC_protein 4.914
Toseland 4.813
ProMoST 5.054
Dawson 4.902
Bjellqvist 5.041
Wikipedia 4.787
Rodwell 4.8
Grimsley 4.736
Solomon 4.902
Lehninger 4.851
Nozaki 5.016
DTASelect 5.194
Thurlkill 4.825
EMBOSS 4.813
Sillero 5.08
Patrickios 4.177
IPC_peptide 4.902
IPC2_peptide 5.067
IPC2.peptide.svr19 5.068
Protein with the highest isoelectric point:
>tr|Q20HX9|Q20HX9_9POLY Minor capsid protein OS=Pyrrhula pyrrhula polyomavirus 1 OX=1891751 PE=3 SV=1
MM1 pKa = 7.56 ALAIWRR7 pKa = 11.84 EE8 pKa = 3.97 QIDD11 pKa = 3.93 YY12 pKa = 10.33 LFPGINWLATNIHH25 pKa = 6.16 YY26 pKa = 10.56 LDD28 pKa = 4.24 PLHH31 pKa = 6.95 WARR34 pKa = 11.84 SLFNQVGRR42 pKa = 11.84 ALWNQIDD49 pKa = 3.6 QRR51 pKa = 11.84 VRR53 pKa = 11.84 DD54 pKa = 3.97 GLIGGAAQAAGAAGAEE70 pKa = 4.22 VAIRR74 pKa = 11.84 QSRR77 pKa = 11.84 ALYY80 pKa = 10.22 DD81 pKa = 3.4 VLARR85 pKa = 11.84 AMEE88 pKa = 4.06 TARR91 pKa = 11.84 WTIHH95 pKa = 5.85 SSYY98 pKa = 10.67 TSAEE102 pKa = 3.61 EE103 pKa = 4.12 TYY105 pKa = 10.74 RR106 pKa = 11.84 NLRR109 pKa = 11.84 EE110 pKa = 4.19 YY111 pKa = 10.53 YY112 pKa = 10.41 AQLPVDD118 pKa = 4.55 AGRR121 pKa = 11.84 PAYY124 pKa = 9.88 RR125 pKa = 11.84 RR126 pKa = 11.84 RR127 pKa = 11.84 LLGLTEE133 pKa = 3.92 QSSWDD138 pKa = 3.51 HH139 pKa = 5.8 GRR141 pKa = 11.84 GPTTSTTEE149 pKa = 3.97 PKK151 pKa = 10.62 AKK153 pKa = 10.33 AIEE156 pKa = 4.24 EE157 pKa = 4.12 ASKK160 pKa = 10.58 EE161 pKa = 4.05 PPEE164 pKa = 4.63 SGEE167 pKa = 4.13 HH168 pKa = 5.01 VQDD171 pKa = 3.8 YY172 pKa = 8.43 PPPGGAHH179 pKa = 5.41 QRR181 pKa = 11.84 HH182 pKa = 5.67 APDD185 pKa = 3.0 WLLPLLLGLYY195 pKa = 10.7 GDD197 pKa = 5.19 LTPEE201 pKa = 3.55 WRR203 pKa = 11.84 SQLKK207 pKa = 9.25 QLGYY211 pKa = 10.8 GSQKK215 pKa = 10.34 RR216 pKa = 11.84 KK217 pKa = 9.48 RR218 pKa = 11.84 QLSPTPASPQADD230 pKa = 3.43 SKK232 pKa = 11.05 RR233 pKa = 11.84 RR234 pKa = 11.84 NRR236 pKa = 11.84 STRR239 pKa = 11.84 RR240 pKa = 11.84 KK241 pKa = 9.56 NRR243 pKa = 11.84 PP244 pKa = 2.95
Molecular weight: 27.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.842
IPC2_protein 9.385
IPC_protein 9.853
Toseland 9.78
ProMoST 9.692
Dawson 10.087
Bjellqvist 9.838
Wikipedia 10.335
Rodwell 10.204
Grimsley 10.204
Solomon 10.131
Lehninger 10.072
Nozaki 9.75
DTASelect 9.853
Thurlkill 9.897
EMBOSS 10.218
Sillero 9.999
Patrickios 5.283
IPC_peptide 10.116
IPC2_peptide 8.595
IPC2.peptide.svr19 8.44
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1939
166
612
323.2
35.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.664 ± 1.445
1.392 ± 0.453
5.725 ± 0.662
5.931 ± 0.462
2.527 ± 0.507
8.045 ± 1.038
2.269 ± 0.384
3.92 ± 0.51
4.126 ± 0.587
10.057 ± 0.714
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.908 ± 0.333
3.559 ± 0.432
7.684 ± 1.336
4.899 ± 0.326
6.601 ± 0.865
6.962 ± 0.856
6.756 ± 0.494
4.642 ± 0.713
1.289 ± 0.417
3.043 ± 0.463
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here