Motilimonas pumilua

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadales genera incertae sedis; Motilimonas

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4087 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A418YH71|A0A418YH71_9GAMM Uncharacterized protein OS=Motilimonas pumilua OX=2303987 GN=D1Z90_05670 PE=4 SV=1
MM1 pKa = 8.12LMFKK5 pKa = 10.42KK6 pKa = 10.63APLYY10 pKa = 10.31LALSAAIALVGCGGSGSSSDD30 pKa = 4.42DD31 pKa = 3.28SDD33 pKa = 3.91GGSTSVEE40 pKa = 3.86PTLAPVVTPSPAPVTTATEE59 pKa = 3.94PRR61 pKa = 11.84SVKK64 pKa = 10.5GVINAPGLTDD74 pKa = 3.25SSVFKK79 pKa = 10.8ISLVSLNAAGEE90 pKa = 4.08LVQQFEE96 pKa = 4.48EE97 pKa = 4.28QLQQTMGQVLFDD109 pKa = 3.97SAIQLASDD117 pKa = 4.07GGSLVVTASSDD128 pKa = 3.4GYY130 pKa = 11.35ASYY133 pKa = 11.57SKK135 pKa = 9.83TFDD138 pKa = 3.1FDD140 pKa = 3.49AASDD144 pKa = 4.12FNSEE148 pKa = 4.14LQVNLQQVNQVVVQKK163 pKa = 10.82GNTIARR169 pKa = 11.84SGLRR173 pKa = 11.84QDD175 pKa = 3.62TFSFAVHH182 pKa = 6.55KK183 pKa = 9.49MPSGRR188 pKa = 11.84SIVVDD193 pKa = 3.6GNGPVPRR200 pKa = 11.84NSLMEE205 pKa = 4.84EE206 pKa = 3.52IRR208 pKa = 11.84IDD210 pKa = 3.44IPADD214 pKa = 3.32SLPASTSALSGRR226 pKa = 11.84IANYY230 pKa = 10.5DD231 pKa = 3.26PTDD234 pKa = 3.82AEE236 pKa = 4.11EE237 pKa = 5.13SEE239 pKa = 4.74AFPGAYY245 pKa = 8.96RR246 pKa = 11.84DD247 pKa = 3.61SDD249 pKa = 3.8GNTLVSVAFSFAEE262 pKa = 4.03IRR264 pKa = 11.84DD265 pKa = 3.58QDD267 pKa = 3.77GQTLSGAIARR277 pKa = 11.84AAEE280 pKa = 4.0QGEE283 pKa = 4.49LVARR287 pKa = 11.84NAAPTMITRR296 pKa = 11.84TIPAGSCASLEE307 pKa = 4.0QLQDD311 pKa = 3.5ANSDD315 pKa = 3.34KK316 pKa = 11.41AGFQIPVYY324 pKa = 9.49TYY326 pKa = 11.03NPNAGDD332 pKa = 3.87WDD334 pKa = 4.13LLGYY338 pKa = 11.06GDD340 pKa = 4.7VFNAADD346 pKa = 4.23DD347 pKa = 4.74SIVPDD352 pKa = 3.51NQQVFDD358 pKa = 4.68CPADD362 pKa = 3.42NYY364 pKa = 9.86YY365 pKa = 11.12LEE367 pKa = 4.77VVVSNDD373 pKa = 3.81DD374 pKa = 4.56FLRR377 pKa = 11.84DD378 pKa = 3.47WWNLDD383 pKa = 3.31YY384 pKa = 10.87PLLFDD389 pKa = 4.89TPVEE393 pKa = 3.84LCANVKK399 pKa = 9.91LLNPSGNPVAGSYY412 pKa = 10.77VFLSDD417 pKa = 3.43PSGNRR422 pKa = 11.84SFSDD426 pKa = 3.85TYY428 pKa = 11.61AVTDD432 pKa = 4.12GQGSVALSTTLLNPDD447 pKa = 3.03NTQRR451 pKa = 11.84TASLRR456 pKa = 11.84HH457 pKa = 5.86WGYY460 pKa = 11.1QSGTYY465 pKa = 9.34LASDD469 pKa = 3.47IQLSEE474 pKa = 4.09QEE476 pKa = 4.28SCGEE480 pKa = 4.02VQVITVQKK488 pKa = 10.57PDD490 pKa = 3.29MCSVSGTVTRR500 pKa = 11.84AAGGSTDD507 pKa = 4.2EE508 pKa = 4.18NSGWLYY514 pKa = 11.01AFPTNTEE521 pKa = 3.72GYY523 pKa = 8.67MNFGFTQAQANGQYY537 pKa = 11.32SMDD540 pKa = 3.82VEE542 pKa = 4.5CDD544 pKa = 2.65IDD546 pKa = 3.79YY547 pKa = 11.18QFYY550 pKa = 9.56TFSDD554 pKa = 5.17FYY556 pKa = 11.33QGINAEE562 pKa = 4.13QEE564 pKa = 4.16AQAKK568 pKa = 6.6TFNVNGTVSGFEE580 pKa = 4.07TSDD583 pKa = 4.02DD584 pKa = 3.51GDD586 pKa = 3.68KK587 pKa = 11.28AILQDD592 pKa = 5.26AEE594 pKa = 4.24IQNLAPLAILTGHH607 pKa = 7.38IMPQGQSMSFLLMDD621 pKa = 4.55FDD623 pKa = 4.71FDD625 pKa = 4.44YY626 pKa = 10.81PVSYY630 pKa = 10.58RR631 pKa = 11.84LEE633 pKa = 4.04FTDD636 pKa = 3.27MNGAVVASVEE646 pKa = 4.04GTAANEE652 pKa = 3.5IDD654 pKa = 4.34YY655 pKa = 10.94DD656 pKa = 4.27SEE658 pKa = 4.68DD659 pKa = 3.32MGAAPFTLPTDD670 pKa = 4.25LPLGSYY676 pKa = 9.69SVSGILTDD684 pKa = 3.35ATGNQGSASGIIGVTEE700 pKa = 3.81

Molecular weight: 74.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A418YE75|A0A418YE75_9GAMM DUF2867 domain-containing protein OS=Motilimonas pumilua OX=2303987 GN=D1Z90_10935 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.14RR12 pKa = 11.84KK13 pKa = 9.13RR14 pKa = 11.84SHH16 pKa = 6.2GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 6.8ATKK25 pKa = 9.91GGRR28 pKa = 11.84QVVARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.48GRR39 pKa = 11.84TRR41 pKa = 11.84LSAA44 pKa = 3.71

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4087

0

4087

1321219

26

4334

323.3

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.251 ± 0.039

1.107 ± 0.013

5.42 ± 0.039

5.821 ± 0.034

4.109 ± 0.027

6.604 ± 0.028

2.377 ± 0.022

5.967 ± 0.031

5.152 ± 0.035

10.684 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.461 ± 0.016

4.062 ± 0.026

4.076 ± 0.025

5.803 ± 0.044

4.219 ± 0.025

6.528 ± 0.031

5.147 ± 0.032

6.834 ± 0.031

1.32 ± 0.018

3.059 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski