Torque teno virus (isolate Human/Japan/TRM1/1999) (TTV) (Torque teno virus genotype 1a)
Average proteome isoelectric point is 8.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9QUB8|CAPSD_TTVV2 Capsid protein OS=Torque teno virus (isolate Human/Japan/TRM1/1999) OX=486275 GN=ORF1 PE=3 SV=1
MM1 pKa = 7.71 FIGRR5 pKa = 11.84 HH6 pKa = 4.04 YY7 pKa = 10.25 RR8 pKa = 11.84 KK9 pKa = 9.85 KK10 pKa = 10.47 RR11 pKa = 11.84 ALSLCAVRR19 pKa = 11.84 TTKK22 pKa = 10.42 KK23 pKa = 10.35 ACKK26 pKa = 9.85 LLILMWTPPRR36 pKa = 11.84 NDD38 pKa = 3.09 QQYY41 pKa = 11.36 LNWQWYY47 pKa = 9.15 SSILSSHH54 pKa = 6.8 AAMCGCPDD62 pKa = 3.41 AVAHH66 pKa = 6.17 FNHH69 pKa = 6.91 LASVLRR75 pKa = 11.84 APQNPPPPGPQRR87 pKa = 11.84 NLPLRR92 pKa = 11.84 RR93 pKa = 11.84 LPALPAAPEE102 pKa = 4.25 APGDD106 pKa = 3.71 RR107 pKa = 11.84 APWPMAGGAEE117 pKa = 4.36 GEE119 pKa = 4.4 DD120 pKa = 3.77 GGAGGDD126 pKa = 3.77 ADD128 pKa = 3.99 HH129 pKa = 7.08 GGAAGGPEE137 pKa = 4.48 DD138 pKa = 5.9 ADD140 pKa = 4.23 LLDD143 pKa = 4.11 AVAAAEE149 pKa = 4.19 TT150 pKa = 3.88
Molecular weight: 15.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.877
IPC2_protein 6.912
IPC_protein 7.132
Toseland 6.898
ProMoST 7.424
Dawson 7.717
Bjellqvist 7.732
Wikipedia 7.644
Rodwell 7.702
Grimsley 6.956
Solomon 7.834
Lehninger 7.863
Nozaki 8.112
DTASelect 7.863
Thurlkill 7.892
EMBOSS 7.98
Sillero 8.17
Patrickios 4.38
IPC_peptide 7.834
IPC2_peptide 7.541
IPC2.peptide.svr19 7.427
Protein with the highest isoelectric point:
>sp|Q9QUB8|CAPSD_TTVV2 Capsid protein OS=Torque teno virus (isolate Human/Japan/TRM1/1999) OX=486275 GN=ORF1 PE=3 SV=1
MM1 pKa = 7.9 AYY3 pKa = 9.87 GWWRR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 WRR14 pKa = 11.84 RR15 pKa = 11.84 WRR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 PWRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 WRR26 pKa = 11.84 TRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 PARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 GRR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 NVRR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 GGRR50 pKa = 11.84 WRR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 YY55 pKa = 9.81 RR56 pKa = 11.84 RR57 pKa = 11.84 WKK59 pKa = 9.23 RR60 pKa = 11.84 KK61 pKa = 6.93 GRR63 pKa = 11.84 RR64 pKa = 11.84 RR65 pKa = 11.84 KK66 pKa = 8.81 KK67 pKa = 9.54 AKK69 pKa = 9.99 IIIRR73 pKa = 11.84 QWQPNYY79 pKa = 10.06 RR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 11.84 CNIVGYY88 pKa = 10.09 IPVLICGEE96 pKa = 4.13 NTVSRR101 pKa = 11.84 NYY103 pKa = 8.79 ATHH106 pKa = 6.96 SDD108 pKa = 3.57 DD109 pKa = 3.58 TNYY112 pKa = 10.32 PGPFGGGMTTDD123 pKa = 3.27 KK124 pKa = 10.02 FTLRR128 pKa = 11.84 ILYY131 pKa = 10.02 DD132 pKa = 3.34 EE133 pKa = 5.02 YY134 pKa = 11.51 KK135 pKa = 10.75 RR136 pKa = 11.84 FMNYY140 pKa = 6.42 WTASNEE146 pKa = 3.86 DD147 pKa = 3.84 LDD149 pKa = 3.9 LCRR152 pKa = 11.84 YY153 pKa = 10.15 LGVNLYY159 pKa = 9.22 FFRR162 pKa = 11.84 HH163 pKa = 6.39 PDD165 pKa = 2.59 VDD167 pKa = 5.04 FIIKK171 pKa = 9.79 INTMPPFLDD180 pKa = 3.76 TEE182 pKa = 4.24 LTAPSIHH189 pKa = 7.03 PGMLALDD196 pKa = 3.83 KK197 pKa = 10.25 RR198 pKa = 11.84 ARR200 pKa = 11.84 WIPSLKK206 pKa = 10.18 SRR208 pKa = 11.84 PGKK211 pKa = 9.5 KK212 pKa = 9.19 HH213 pKa = 5.39 YY214 pKa = 10.49 IKK216 pKa = 10.45 IRR218 pKa = 11.84 VEE220 pKa = 3.9 APKK223 pKa = 10.2 MFTDD227 pKa = 3.01 KK228 pKa = 10.35 WYY230 pKa = 9.23 PQTDD234 pKa = 3.67 LCDD237 pKa = 3.56 MVLLTVYY244 pKa = 9.37 ATTADD249 pKa = 3.37 MQYY252 pKa = 10.91 PFGSPLTDD260 pKa = 2.83 SVVVNFQVLQSMYY273 pKa = 10.16 DD274 pKa = 3.4 QNISILPTEE283 pKa = 4.11 KK284 pKa = 10.31 SKK286 pKa = 10.06 RR287 pKa = 11.84 TQLHH291 pKa = 6.62 DD292 pKa = 4.39 NITRR296 pKa = 11.84 YY297 pKa = 7.87 TPFYY301 pKa = 10.27 NTTQTIAQLKK311 pKa = 8.68 PFVDD315 pKa = 4.03 AGNVTPVSPTTTWGSYY331 pKa = 10.57 INTTKK336 pKa = 9.75 FTTTATTTYY345 pKa = 8.82 TYY347 pKa = 10.58 PGTTTTTVTMLTCNDD362 pKa = 2.26 SWYY365 pKa = 10.86 RR366 pKa = 11.84 GTVYY370 pKa = 10.81 NNQISQLPKK379 pKa = 9.92 KK380 pKa = 10.32 AAEE383 pKa = 4.56 FYY385 pKa = 11.18 SKK387 pKa = 9.88 ATKK390 pKa = 9.42 TLLGDD395 pKa = 3.95 TFTTEE400 pKa = 4.94 DD401 pKa = 3.61 YY402 pKa = 10.25 TLEE405 pKa = 4.12 YY406 pKa = 10.59 HH407 pKa = 6.55 GGLYY411 pKa = 10.46 SSIWLSAGRR420 pKa = 11.84 SYY422 pKa = 11.53 FEE424 pKa = 4.21 TPGVYY429 pKa = 9.67 TDD431 pKa = 3.15 IKK433 pKa = 10.75 YY434 pKa = 10.96 NPFTDD439 pKa = 3.29 RR440 pKa = 11.84 GEE442 pKa = 4.91 GNMLWIDD449 pKa = 3.58 WLSKK453 pKa = 11.24 KK454 pKa = 10.29 NMNYY458 pKa = 10.8 DD459 pKa = 3.49 KK460 pKa = 11.15 VQSKK464 pKa = 10.38 CLISDD469 pKa = 4.25 LPLWAAAYY477 pKa = 9.96 GYY479 pKa = 10.96 VEE481 pKa = 5.33 FCAKK485 pKa = 9.33 STGDD489 pKa = 3.65 QNIHH493 pKa = 5.93 MNAKK497 pKa = 10.04 LLIRR501 pKa = 11.84 SPFTDD506 pKa = 3.55 PQLLVHH512 pKa = 6.69 TDD514 pKa = 3.3 PTKK517 pKa = 11.11 GFVPYY522 pKa = 10.54 SLNFGNGKK530 pKa = 8.58 MPGGSSNVPIRR541 pKa = 11.84 MRR543 pKa = 11.84 AKK545 pKa = 8.79 WYY547 pKa = 7.13 PTLFHH552 pKa = 5.65 QQEE555 pKa = 4.27 VLEE558 pKa = 4.87 ALAQSGPFAYY568 pKa = 10.25 HH569 pKa = 6.47 SDD571 pKa = 3.2 IKK573 pKa = 10.84 KK574 pKa = 10.15 VSLGMKK580 pKa = 9.93 YY581 pKa = 9.58 RR582 pKa = 11.84 FKK584 pKa = 10.37 WIWGGNPVRR593 pKa = 11.84 QQVVRR598 pKa = 11.84 NPCKK602 pKa = 9.17 EE603 pKa = 4.1 THH605 pKa = 6.01 SSGNRR610 pKa = 11.84 VPRR613 pKa = 11.84 SLQIVDD619 pKa = 3.88 PKK621 pKa = 11.07 YY622 pKa = 10.83 NSPEE626 pKa = 4.2 LTFHH630 pKa = 5.72 TWDD633 pKa = 3.33 FRR635 pKa = 11.84 RR636 pKa = 11.84 GLFGPKK642 pKa = 9.81 AIQRR646 pKa = 11.84 MQQQPTTTDD655 pKa = 2.42 IFSAGRR661 pKa = 11.84 KK662 pKa = 8.47 RR663 pKa = 11.84 PRR665 pKa = 11.84 RR666 pKa = 11.84 DD667 pKa = 2.94 TEE669 pKa = 4.53 VYY671 pKa = 10.05 HH672 pKa = 6.82 SSQEE676 pKa = 4.27 GEE678 pKa = 4.15 QKK680 pKa = 10.68 EE681 pKa = 4.48 SLLFLPVKK689 pKa = 10.17 LLRR692 pKa = 11.84 RR693 pKa = 11.84 VPPWEE698 pKa = 4.86 DD699 pKa = 3.18 SQQEE703 pKa = 4.11 EE704 pKa = 4.7 SGSQSSEE711 pKa = 3.98 EE712 pKa = 3.99 EE713 pKa = 4.26 TQTVSQQLKK722 pKa = 9.41 QQLQQQRR729 pKa = 11.84 ILGVKK734 pKa = 9.95 LRR736 pKa = 11.84 LLFNQVQKK744 pKa = 10.59 IQQNQDD750 pKa = 2.75 INPTLLPRR758 pKa = 11.84 GGDD761 pKa = 3.42 LASLFQIAPP770 pKa = 3.53
Molecular weight: 90.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.299
IPC2_protein 9.692
IPC_protein 10.204
Toseland 10.277
ProMoST 10.014
Dawson 10.482
Bjellqvist 10.189
Wikipedia 10.672
Rodwell 10.774
Grimsley 10.555
Solomon 10.511
Lehninger 10.467
Nozaki 10.277
DTASelect 10.175
Thurlkill 10.321
EMBOSS 10.687
Sillero 10.394
Patrickios 10.306
IPC_peptide 10.511
IPC2_peptide 9.048
IPC2.peptide.svr19 8.656
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1496
150
770
374.0
42.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.489 ± 3.203
1.471 ± 0.444
4.947 ± 0.235
3.61 ± 0.193
2.941 ± 0.768
7.487 ± 1.081
1.939 ± 0.301
3.409 ± 0.858
5.348 ± 0.67
7.487 ± 0.483
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.872 ± 0.294
4.412 ± 0.237
9.158 ± 1.935
5.214 ± 0.56
8.824 ± 0.925
6.952 ± 1.649
6.551 ± 1.699
3.61 ± 0.897
2.607 ± 0.298
3.676 ± 0.992
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here