Corallococcus sp. CAG:1435
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1300 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R5W5F6|R5W5F6_9DELT Lon protease OS=Corallococcus sp. CAG:1435 OX=1262867 GN=lon PE=2 SV=1
MM1 pKa = 7.63 PAGNVTATANYY12 pKa = 9.74 EE13 pKa = 4.25 FIDD16 pKa = 3.6 YY17 pKa = 8.44 TVTVNGGKK25 pKa = 9.73 AQVNDD30 pKa = 4.22 GVASASVKK38 pKa = 10.13 AHH40 pKa = 6.18 YY41 pKa = 10.52 GDD43 pKa = 3.95 TVTITAEE50 pKa = 4.07 VPVGKK55 pKa = 10.07 EE56 pKa = 3.39 FVMWSFNGLVPDD68 pKa = 5.49 GLTLTNKK75 pKa = 9.79 EE76 pKa = 4.28 LTFTMPANAVTATAEE91 pKa = 3.96 LRR93 pKa = 11.84 DD94 pKa = 3.9 LVKK97 pKa = 10.93 SSVTVQGGVAQVGDD111 pKa = 4.28 GEE113 pKa = 5.16 LEE115 pKa = 4.21 TSLEE119 pKa = 4.34 AYY121 pKa = 10.5 CGDD124 pKa = 3.75 TVTLTAYY131 pKa = 10.33 QYY133 pKa = 10.25 TDD135 pKa = 2.8 KK136 pKa = 11.17 TFVKK140 pKa = 8.43 WTIEE144 pKa = 4.5 GIDD147 pKa = 3.75 TEE149 pKa = 5.24 GLDD152 pKa = 3.36 LTQRR156 pKa = 11.84 EE157 pKa = 4.69 LTFTMPEE164 pKa = 3.79 NDD166 pKa = 3.13 VTATAEE172 pKa = 4.15 FEE174 pKa = 4.31 NVPTEE179 pKa = 3.96 LTFEE183 pKa = 4.72 GNTATSGEE191 pKa = 4.25 LWYY194 pKa = 11.18 VNYY197 pKa = 10.09 DD198 pKa = 3.43 SKK200 pKa = 11.59 SFRR203 pKa = 11.84 LPLGSGQDD211 pKa = 3.18 SCTYY215 pKa = 10.05 TIEE218 pKa = 4.31 LSNSAFTFKK227 pKa = 10.99 VYY229 pKa = 10.69 GSDD232 pKa = 3.5 GEE234 pKa = 4.49 VYY236 pKa = 9.98 IDD238 pKa = 3.73 EE239 pKa = 4.58 NGEE242 pKa = 4.23 GVKK245 pKa = 10.58 SITIDD250 pKa = 3.41 RR251 pKa = 11.84 EE252 pKa = 3.99 DD253 pKa = 3.57 NYY255 pKa = 10.72 TIEE258 pKa = 4.45 VINTGTAANCTLTVTQII275 pKa = 3.31
Molecular weight: 29.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.793
IPC2_protein 3.986
IPC_protein 3.948
Toseland 3.757
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.795
Rodwell 3.77
Grimsley 3.668
Solomon 3.897
Lehninger 3.859
Nozaki 4.012
DTASelect 4.177
Thurlkill 3.783
EMBOSS 3.808
Sillero 4.05
Patrickios 1.888
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.952
Protein with the highest isoelectric point:
>tr|R5WHI6|R5WHI6_9DELT Cation-transporting ATPase P-type OS=Corallococcus sp. CAG:1435 OX=1262867 GN=BN495_00525 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.67 QPKK8 pKa = 9.11 KK9 pKa = 7.52 RR10 pKa = 11.84 QRR12 pKa = 11.84 SKK14 pKa = 9.17 VHH16 pKa = 5.94 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MKK23 pKa = 7.97 TTGGRR28 pKa = 11.84 RR29 pKa = 11.84 TLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 NKK37 pKa = 9.66 GRR39 pKa = 11.84 HH40 pKa = 5.02 SLSAA44 pKa = 3.67
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1300
0
1300
407049
34
2964
313.1
34.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.63 ± 0.07
2.005 ± 0.036
5.587 ± 0.052
5.967 ± 0.066
4.763 ± 0.058
6.681 ± 0.067
1.636 ± 0.037
5.935 ± 0.068
6.908 ± 0.074
9.121 ± 0.084
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.376 ± 0.037
4.909 ± 0.047
3.028 ± 0.033
3.854 ± 0.053
4.074 ± 0.068
6.062 ± 0.058
6.062 ± 0.1
8.654 ± 0.067
0.789 ± 0.024
3.952 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here