Lactobacillus senioris DSM 24302 = JCM 17472
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1539 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R2CZI9|A0A0R2CZI9_9LACO Bacteriophage integrase OS=Lactobacillus senioris DSM 24302 = JCM 17472 OX=1423802 GN=FC56_GL000311 PE=4 SV=1
MM1 pKa = 7.0 ITAQVIYY8 pKa = 9.07 ATITGNNEE16 pKa = 4.06 DD17 pKa = 3.59 IADD20 pKa = 4.45 IITEE24 pKa = 4.05 RR25 pKa = 11.84 FEE27 pKa = 5.23 DD28 pKa = 3.4 RR29 pKa = 11.84 GIDD32 pKa = 3.64 VNQTEE37 pKa = 4.62 ISQADD42 pKa = 3.06 ASVFLEE48 pKa = 3.81 CDD50 pKa = 2.81 ICVVCPYY57 pKa = 10.11 TYY59 pKa = 10.79 DD60 pKa = 3.34 EE61 pKa = 4.73 GALPEE66 pKa = 4.65 EE67 pKa = 4.81 GLDD70 pKa = 4.72 FYY72 pKa = 11.87 DD73 pKa = 6.1 DD74 pKa = 3.95 LQEE77 pKa = 4.72 LNLSGKK83 pKa = 10.27 LYY85 pKa = 10.77 GVAGSGDD92 pKa = 3.35 TYY94 pKa = 11.32 YY95 pKa = 11.11 EE96 pKa = 3.83 EE97 pKa = 4.52 FFNVAVDD104 pKa = 3.49 KK105 pKa = 11.18 FEE107 pKa = 4.71 QAFQKK112 pKa = 10.91 AGATKK117 pKa = 9.02 ATEE120 pKa = 4.07 SVKK123 pKa = 10.66 INLEE127 pKa = 3.87 PDD129 pKa = 3.24 QVDD132 pKa = 3.57 IEE134 pKa = 4.37 ALDD137 pKa = 3.98 EE138 pKa = 4.32 FTDD141 pKa = 4.12 DD142 pKa = 6.35 LILAAQKK149 pKa = 10.32 QATEE153 pKa = 3.9
Molecular weight: 16.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.617
IPC_protein 3.592
Toseland 3.389
ProMoST 3.745
Dawson 3.579
Bjellqvist 3.783
Wikipedia 3.503
Rodwell 3.427
Grimsley 3.3
Solomon 3.567
Lehninger 3.528
Nozaki 3.706
DTASelect 3.897
Thurlkill 3.439
EMBOSS 3.516
Sillero 3.719
Patrickios 0.947
IPC_peptide 3.567
IPC2_peptide 3.694
IPC2.peptide.svr19 3.701
Protein with the highest isoelectric point:
>tr|A0A0R2CRW1|A0A0R2CRW1_9LACO Phosphomethylpyrimidine kinase OS=Lactobacillus senioris DSM 24302 = JCM 17472 OX=1423802 GN=FC56_GL001466 PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.19 KK9 pKa = 7.37 RR10 pKa = 11.84 HH11 pKa = 5.5 RR12 pKa = 11.84 ARR14 pKa = 11.84 VHH16 pKa = 5.84 GFRR19 pKa = 11.84 KK20 pKa = 10.03 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.01 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.39 GRR39 pKa = 11.84 KK40 pKa = 8.52 VLSAA44 pKa = 4.05
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1539
0
1539
461827
44
1687
300.1
33.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.988 ± 0.059
0.359 ± 0.011
5.941 ± 0.059
5.636 ± 0.071
4.13 ± 0.046
6.671 ± 0.061
1.937 ± 0.029
7.271 ± 0.052
6.241 ± 0.063
9.582 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.695 ± 0.029
5.258 ± 0.047
3.626 ± 0.032
4.822 ± 0.056
3.853 ± 0.049
5.935 ± 0.058
6.268 ± 0.045
7.28 ± 0.047
1.026 ± 0.026
3.481 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here