candidate division MSBL1 archaeon SCGC-AAA385D11

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 299 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133VN62|A0A133VN62_9EURY TDP-4-oxo-6-deoxy-D-glucose aminotransferase OS=candidate division MSBL1 archaeon SCGC-AAA385D11 OX=1698286 GN=AKJ58_01555 PE=3 SV=1
NN1 pKa = 7.87NIVQYY6 pKa = 11.56NNIYY10 pKa = 10.89NNTNNGIQHH19 pKa = 6.72NGTGTLDD26 pKa = 3.15ATYY29 pKa = 10.36NWWGDD34 pKa = 3.37SSGPSGVGPGEE45 pKa = 3.96GDD47 pKa = 3.08AVSEE51 pKa = 4.08NVKK54 pKa = 10.43YY55 pKa = 10.85NPWLLNPFEE64 pKa = 4.68EE65 pKa = 4.55PQTYY69 pKa = 10.16YY70 pKa = 11.09DD71 pKa = 3.35HH72 pKa = 7.53CEE74 pKa = 3.89ALNSGWNLISTPKK87 pKa = 10.21ILEE90 pKa = 4.12NDD92 pKa = 4.73DD93 pKa = 3.43ISEE96 pKa = 4.29MVADD100 pKa = 4.42MDD102 pKa = 4.34NLQHH106 pKa = 6.68VYY108 pKa = 10.01RR109 pKa = 11.84YY110 pKa = 9.19EE111 pKa = 3.81NSGWIEE117 pKa = 3.95DD118 pKa = 3.58NAAYY122 pKa = 9.71GQEE125 pKa = 3.88PLYY128 pKa = 11.22ASFVKK133 pKa = 10.67VKK135 pKa = 10.45EE136 pKa = 4.02EE137 pKa = 4.06AGSIGIGYY145 pKa = 8.68NWSTDD150 pKa = 3.69PQDD153 pKa = 3.68VPPSRR158 pKa = 11.84DD159 pKa = 3.53LPEE162 pKa = 4.39GWSMIGPSIDD172 pKa = 3.59PSVEE176 pKa = 3.63PSVPADD182 pKa = 3.49EE183 pKa = 5.28FLDD186 pKa = 4.03SVSEE190 pKa = 4.38SYY192 pKa = 9.21STLFSPDD199 pKa = 3.12FNLNSWTVTTKK210 pKa = 9.81TAADD214 pKa = 3.71EE215 pKa = 4.14NVLPFEE221 pKa = 4.83GYY223 pKa = 9.03WIFMEE228 pKa = 5.2SLDD231 pKa = 3.96TLAGRR236 pKa = 11.84TTT238 pKa = 3.25

Molecular weight: 26.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133VNS7|A0A133VNS7_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA385D11 OX=1698286 GN=AKJ58_00915 PE=4 SV=1
MM1 pKa = 6.16MTVDD5 pKa = 3.79VFHH8 pKa = 7.26SKK10 pKa = 10.7DD11 pKa = 3.28DD12 pKa = 3.85SAWCVRR18 pKa = 11.84VRR20 pKa = 11.84SIGQNRR26 pKa = 11.84VVSRR30 pKa = 11.84HH31 pKa = 4.36RR32 pKa = 11.84TKK34 pKa = 10.48KK35 pKa = 9.62AAIRR39 pKa = 11.84RR40 pKa = 11.84AKK42 pKa = 10.47KK43 pKa = 8.73EE44 pKa = 3.78AKK46 pKa = 10.3ALGARR51 pKa = 11.84IDD53 pKa = 3.75VYY55 pKa = 11.32KK56 pKa = 10.71KK57 pKa = 10.6DD58 pKa = 3.42GTLQRR63 pKa = 11.84TLNYY67 pKa = 9.9GWQLL71 pKa = 3.16

Molecular weight: 8.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

299

0

299

72907

57

2061

243.8

27.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.334 ± 0.168

1.167 ± 0.084

5.726 ± 0.104

9.605 ± 0.217

3.788 ± 0.102

7.552 ± 0.131

1.658 ± 0.063

6.835 ± 0.135

7.869 ± 0.193

8.981 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.068

3.797 ± 0.162

4.028 ± 0.086

2.266 ± 0.065

5.935 ± 0.183

6.436 ± 0.142

4.786 ± 0.236

6.984 ± 0.127

1.121 ± 0.066

2.946 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski