Amino acid dipepetide frequency for Streptomyces sp. PTY087I2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.64AlaAla: 21.64 ± 0.136	1.021AlaCys: 1.021 ± 0.022	8.472AlaAsp: 8.472 ± 0.075	9.147AlaGlu: 9.147 ± 0.082	3.565AlaPhe: 3.565 ± 0.039	13.072AlaGly: 13.072 ± 0.103	2.803AlaHis: 2.803 ± 0.035	3.419AlaIle: 3.419 ± 0.041	2.912AlaLys: 2.912 ± 0.048	14.704AlaLeu: 14.704 ± 0.106	2.448AlaMet: 2.448 ± 0.036	1.883AlaAsn: 1.883 ± 0.034	7.715AlaPro: 7.715 ± 0.078	3.553AlaGln: 3.553 ± 0.043	10.105AlaArg: 10.105 ± 0.082	6.144AlaSer: 6.144 ± 0.058	7.093AlaThr: 7.093 ± 0.068	12.29AlaVal: 12.29 ± 0.082	1.78AlaTrp: 1.78 ± 0.032	2.844AlaTyr: 2.844 ± 0.033	0.0AlaXaa: 0.0 ± 0.0
Cys	1.056CysAla: 1.056 ± 0.022	0.097CysCys: 0.097 ± 0.007	0.457CysAsp: 0.457 ± 0.015	0.388CysGlu: 0.388 ± 0.014	0.216CysPhe: 0.216 ± 0.011	0.95CysGly: 0.95 ± 0.017	0.19CysHis: 0.19 ± 0.01	0.164CysIle: 0.164 ± 0.008	0.102CysLys: 0.102 ± 0.007	0.678CysLeu: 0.678 ± 0.019	0.124CysMet: 0.124 ± 0.007	0.122CysAsn: 0.122 ± 0.007	0.475CysPro: 0.475 ± 0.017	0.153CysGln: 0.153 ± 0.008	0.579CysArg: 0.579 ± 0.019	0.441CysSer: 0.441 ± 0.017	0.504CysThr: 0.504 ± 0.015	0.634CysVal: 0.634 ± 0.018	0.123CysTrp: 0.123 ± 0.006	0.136CysTyr: 0.136 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	7.984AspAla: 7.984 ± 0.063	0.413AspCys: 0.413 ± 0.013	3.608AspAsp: 3.608 ± 0.046	3.981AspGlu: 3.981 ± 0.047	1.63AspPhe: 1.63 ± 0.029	6.449AspGly: 6.449 ± 0.063	1.43AspHis: 1.43 ± 0.022	1.887AspIle: 1.887 ± 0.027	1.164AspLys: 1.164 ± 0.029	6.116AspLeu: 6.116 ± 0.053	0.798AspMet: 0.798 ± 0.019	0.934AspAsn: 0.934 ± 0.021	4.671AspPro: 4.671 ± 0.052	1.483AspGln: 1.483 ± 0.027	5.291AspArg: 5.291 ± 0.055	2.553AspSer: 2.553 ± 0.039	3.264AspThr: 3.264 ± 0.033	4.516AspVal: 4.516 ± 0.047	1.004AspTrp: 1.004 ± 0.023	1.085AspTyr: 1.085 ± 0.023	0.001AspXaa: 0.001 ± 0.001
Glu	7.745GluAla: 7.745 ± 0.088	0.344GluCys: 0.344 ± 0.013	2.933GluAsp: 2.933 ± 0.038	3.762GluGlu: 3.762 ± 0.056	1.493GluPhe: 1.493 ± 0.027	4.63GluGly: 4.63 ± 0.046	1.494GluHis: 1.494 ± 0.025	2.306GluIle: 2.306 ± 0.035	1.648GluLys: 1.648 ± 0.032	6.889GluLeu: 6.889 ± 0.07	0.85GluMet: 0.85 ± 0.019	1.123GluAsn: 1.123 ± 0.023	3.542GluPro: 3.542 ± 0.046	2.265GluGln: 2.265 ± 0.042	5.576GluArg: 5.576 ± 0.058	2.659GluSer: 2.659 ± 0.036	2.985GluThr: 2.985 ± 0.042	4.402GluVal: 4.402 ± 0.046	0.732GluTrp: 0.732 ± 0.018	1.055GluTyr: 1.055 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.72PheAla: 3.72 ± 0.043	0.282PheCys: 0.282 ± 0.011	1.956PheAsp: 1.956 ± 0.032	1.378PheGlu: 1.378 ± 0.027	0.868PhePhe: 0.868 ± 0.022	3.013PheGly: 3.013 ± 0.035	0.596PheHis: 0.596 ± 0.015	0.76PheIle: 0.76 ± 0.018	0.491PheLys: 0.491 ± 0.015	2.57PheLeu: 2.57 ± 0.039	0.398PheMet: 0.398 ± 0.014	0.52PheAsn: 0.52 ± 0.015	1.41PhePro: 1.41 ± 0.026	0.65PheGln: 0.65 ± 0.019	1.871PheArg: 1.871 ± 0.028	1.467PheSer: 1.467 ± 0.025	1.984PheThr: 1.984 ± 0.032	2.13PheVal: 2.13 ± 0.034	0.422PheTrp: 0.422 ± 0.014	0.55PheTyr: 0.55 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	11.25GlyAla: 11.25 ± 0.093	0.83GlyCys: 0.83 ± 0.021	5.334GlyAsp: 5.334 ± 0.049	5.45GlyGlu: 5.45 ± 0.047	2.842GlyPhe: 2.842 ± 0.036	9.17GlyGly: 9.17 ± 0.115	2.256GlyHis: 2.256 ± 0.034	3.473GlyIle: 3.473 ± 0.046	2.548GlyLys: 2.548 ± 0.038	9.421GlyLeu: 9.421 ± 0.076	1.917GlyMet: 1.917 ± 0.03	1.773GlyAsn: 1.773 ± 0.033	5.712GlyPro: 5.712 ± 0.061	2.715GlyGln: 2.715 ± 0.042	7.856GlyArg: 7.856 ± 0.065	5.718GlySer: 5.718 ± 0.061	6.22GlyThr: 6.22 ± 0.057	7.271GlyVal: 7.271 ± 0.062	1.661GlyTrp: 1.661 ± 0.025	2.199GlyTyr: 2.199 ± 0.033	0.0GlyXaa: 0.0 ± 0.0
His	2.576HisAla: 2.576 ± 0.034	0.201HisCys: 0.201 ± 0.008	1.302HisAsp: 1.302 ± 0.023	1.188HisGlu: 1.188 ± 0.021	0.602HisPhe: 0.602 ± 0.018	2.32HisGly: 2.32 ± 0.035	0.67HisHis: 0.67 ± 0.018	0.714HisIle: 0.714 ± 0.018	0.316HisLys: 0.316 ± 0.012	2.402HisLeu: 2.402 ± 0.034	0.312HisMet: 0.312 ± 0.011	0.349HisAsn: 0.349 ± 0.011	1.806HisPro: 1.806 ± 0.027	0.675HisGln: 0.675 ± 0.018	2.194HisArg: 2.194 ± 0.034	0.997HisSer: 0.997 ± 0.022	1.336HisThr: 1.336 ± 0.023	1.538HisVal: 1.538 ± 0.027	0.363HisTrp: 0.363 ± 0.012	0.455HisTyr: 0.455 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	4.9IleAla: 4.9 ± 0.04	0.297IleCys: 0.297 ± 0.012	2.286IleAsp: 2.286 ± 0.033	2.003IleGlu: 2.003 ± 0.033	0.707IlePhe: 0.707 ± 0.02	3.663IleGly: 3.663 ± 0.047	0.625IleHis: 0.625 ± 0.017	0.91IleIle: 0.91 ± 0.024	0.739IleLys: 0.739 ± 0.02	2.419IleLeu: 2.419 ± 0.032	0.435IleMet: 0.435 ± 0.015	0.675IleAsn: 0.675 ± 0.017	1.816IlePro: 1.816 ± 0.032	0.727IleGln: 0.727 ± 0.02	2.214IleArg: 2.214 ± 0.031	1.72IleSer: 1.72 ± 0.031	2.204IleThr: 2.204 ± 0.032	2.811IleVal: 2.811 ± 0.04	0.398IleTrp: 0.398 ± 0.014	0.522IleTyr: 0.522 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	2.989LysAla: 2.989 ± 0.05	0.101LysCys: 0.101 ± 0.007	1.437LysAsp: 1.437 ± 0.032	1.299LysGlu: 1.299 ± 0.027	0.463LysPhe: 0.463 ± 0.015	1.908LysGly: 1.908 ± 0.036	0.452LysHis: 0.452 ± 0.016	0.889LysIle: 0.889 ± 0.022	1.001LysLys: 1.001 ± 0.032	2.073LysLeu: 2.073 ± 0.035	0.395LysMet: 0.395 ± 0.013	0.57LysAsn: 0.57 ± 0.021	1.439LysPro: 1.439 ± 0.029	0.716LysGln: 0.716 ± 0.018	1.447LysArg: 1.447 ± 0.028	1.168LysSer: 1.168 ± 0.024	1.286LysThr: 1.286 ± 0.029	1.871LysVal: 1.871 ± 0.033	0.242LysTrp: 0.242 ± 0.012	0.416LysTyr: 0.416 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	14.949LeuAla: 14.949 ± 0.119	0.814LeuCys: 0.814 ± 0.018	6.746LeuAsp: 6.746 ± 0.061	4.646LeuGlu: 4.646 ± 0.053	2.599LeuPhe: 2.599 ± 0.036	9.237LeuGly: 9.237 ± 0.084	2.273LeuHis: 2.273 ± 0.036	3.441LeuIle: 3.441 ± 0.038	2.044LeuLys: 2.044 ± 0.035	11.48LeuLeu: 11.48 ± 0.116	1.637LeuMet: 1.637 ± 0.03	1.716LeuAsn: 1.716 ± 0.027	6.635LeuPro: 6.635 ± 0.065	2.078LeuGln: 2.078 ± 0.033	8.474LeuArg: 8.474 ± 0.064	5.348LeuSer: 5.348 ± 0.055	6.849LeuThr: 6.849 ± 0.059	8.727LeuVal: 8.727 ± 0.08	1.301LeuTrp: 1.301 ± 0.027	1.832LeuTyr: 1.832 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	2.166MetAla: 2.166 ± 0.031	0.132MetCys: 0.132 ± 0.007	0.853MetAsp: 0.853 ± 0.018	0.772MetGlu: 0.772 ± 0.021	0.441MetPhe: 0.441 ± 0.014	1.307MetGly: 1.307 ± 0.026	0.331MetHis: 0.331 ± 0.011	0.67MetIle: 0.67 ± 0.015	0.452MetLys: 0.452 ± 0.014	1.63MetLeu: 1.63 ± 0.03	0.291MetMet: 0.291 ± 0.012	0.45MetAsn: 0.45 ± 0.015	1.082MetPro: 1.082 ± 0.023	0.433MetGln: 0.433 ± 0.016	1.373MetArg: 1.373 ± 0.024	1.311MetSer: 1.311 ± 0.023	1.526MetThr: 1.526 ± 0.028	1.253MetVal: 1.253 ± 0.026	0.178MetTrp: 0.178 ± 0.009	0.306MetTyr: 0.306 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.206AsnAla: 2.206 ± 0.034	0.147AsnCys: 0.147 ± 0.008	0.974AsnAsp: 0.974 ± 0.022	0.826AsnGlu: 0.826 ± 0.019	0.473AsnPhe: 0.473 ± 0.014	1.888AsnGly: 1.888 ± 0.036	0.373AsnHis: 0.373 ± 0.011	0.643AsnIle: 0.643 ± 0.018	0.418AsnLys: 0.418 ± 0.015	1.612AsnLeu: 1.612 ± 0.03	0.296AsnMet: 0.296 ± 0.013	0.42AsnAsn: 0.42 ± 0.015	1.325AsnPro: 1.325 ± 0.022	0.501AsnGln: 0.501 ± 0.016	1.278AsnArg: 1.278 ± 0.02	0.912AsnSer: 0.912 ± 0.021	1.116AsnThr: 1.116 ± 0.028	1.325AsnVal: 1.325 ± 0.027	0.277AsnTrp: 0.277 ± 0.01	0.398AsnTyr: 0.398 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	9.079ProAla: 9.079 ± 0.091	0.327ProCys: 0.327 ± 0.014	4.836ProAsp: 4.836 ± 0.05	4.723ProGlu: 4.723 ± 0.058	1.582ProPhe: 1.582 ± 0.026	7.064ProGly: 7.064 ± 0.069	1.371ProHis: 1.371 ± 0.022	1.245ProIle: 1.245 ± 0.023	1.284ProLys: 1.284 ± 0.026	5.497ProLeu: 5.497 ± 0.061	1.005ProMet: 1.005 ± 0.019	0.9ProAsn: 0.9 ± 0.022	3.757ProPro: 3.757 ± 0.067	1.723ProGln: 1.723 ± 0.036	3.937ProArg: 3.937 ± 0.049	3.295ProSer: 3.295 ± 0.042	3.237ProThr: 3.237 ± 0.053	5.753ProVal: 5.753 ± 0.057	0.895ProTrp: 0.895 ± 0.019	1.449ProTyr: 1.449 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	3.434GlnAla: 3.434 ± 0.045	0.151GlnCys: 0.151 ± 0.009	1.402GlnAsp: 1.402 ± 0.025	1.491GlnGlu: 1.491 ± 0.026	0.654GlnPhe: 0.654 ± 0.017	2.277GlnGly: 2.277 ± 0.036	0.665GlnHis: 0.665 ± 0.019	1.065GlnIle: 1.065 ± 0.019	0.626GlnLys: 0.626 ± 0.018	2.981GlnLeu: 2.981 ± 0.04	0.466GlnMet: 0.466 ± 0.015	0.518GlnAsn: 0.518 ± 0.016	1.659GlnPro: 1.659 ± 0.04	1.302GlnGln: 1.302 ± 0.035	2.339GlnArg: 2.339 ± 0.04	1.219GlnSer: 1.219 ± 0.022	1.262GlnThr: 1.262 ± 0.022	2.303GlnVal: 2.303 ± 0.029	0.444GlnTrp: 0.444 ± 0.015	0.551GlnTyr: 0.551 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	9.813ArgAla: 9.813 ± 0.079	0.574ArgCys: 0.574 ± 0.017	4.291ArgAsp: 4.291 ± 0.054	4.746ArgGlu: 4.746 ± 0.051	2.319ArgPhe: 2.319 ± 0.031	5.781ArgGly: 5.781 ± 0.057	1.983ArgHis: 1.983 ± 0.034	3.374ArgIle: 3.374 ± 0.036	1.691ArgLys: 1.691 ± 0.03	8.621ArgLeu: 8.621 ± 0.067	1.736ArgMet: 1.736 ± 0.027	1.394ArgAsn: 1.394 ± 0.025	5.071ArgPro: 5.071 ± 0.062	2.15ArgGln: 2.15 ± 0.037	7.441ArgArg: 7.441 ± 0.077	4.365ArgSer: 4.365 ± 0.052	5.834ArgThr: 5.834 ± 0.056	5.554ArgVal: 5.554 ± 0.05	1.342ArgTrp: 1.342 ± 0.026	1.765ArgTyr: 1.765 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	7.066SerAla: 7.066 ± 0.058	0.415SerCys: 0.415 ± 0.013	2.702SerAsp: 2.702 ± 0.033	2.473SerGlu: 2.473 ± 0.033	1.5SerPhe: 1.5 ± 0.024	6.245SerGly: 6.245 ± 0.061	1.002SerHis: 1.002 ± 0.021	1.505SerIle: 1.505 ± 0.029	1.05SerLys: 1.05 ± 0.026	4.859SerLeu: 4.859 ± 0.05	1.059SerMet: 1.059 ± 0.021	0.807SerAsn: 0.807 ± 0.019	3.373SerPro: 3.373 ± 0.043	1.191SerGln: 1.191 ± 0.024	3.7SerArg: 3.7 ± 0.036	2.912SerSer: 2.912 ± 0.046	3.177SerThr: 3.177 ± 0.037	4.357SerVal: 4.357 ± 0.048	0.907SerTrp: 0.907 ± 0.022	1.193SerTyr: 1.193 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	9.009ThrAla: 9.009 ± 0.073	0.391ThrCys: 0.391 ± 0.013	3.626ThrAsp: 3.626 ± 0.042	3.398ThrGlu: 3.398 ± 0.039	1.638ThrPhe: 1.638 ± 0.024	7.008ThrGly: 7.008 ± 0.06	1.148ThrHis: 1.148 ± 0.02	1.681ThrIle: 1.681 ± 0.03	1.162ThrLys: 1.162 ± 0.025	5.73ThrLeu: 5.73 ± 0.05	0.868ThrMet: 0.868 ± 0.019	0.92ThrAsn: 0.92 ± 0.02	4.351ThrPro: 4.351 ± 0.057	1.294ThrGln: 1.294 ± 0.027	3.861ThrArg: 3.861 ± 0.04	3.12ThrSer: 3.12 ± 0.037	3.891ThrThr: 3.891 ± 0.06	6.118ThrVal: 6.118 ± 0.059	0.877ThrTrp: 0.877 ± 0.02	1.361ThrTyr: 1.361 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	10.581ValAla: 10.581 ± 0.081	0.732ValCys: 0.732 ± 0.017	4.826ValAsp: 4.826 ± 0.047	4.736ValGlu: 4.736 ± 0.055	2.322ValPhe: 2.322 ± 0.03	6.338ValGly: 6.338 ± 0.062	1.939ValHis: 1.939 ± 0.029	2.86ValIle: 2.86 ± 0.042	1.746ValLys: 1.746 ± 0.033	9.433ValLeu: 9.433 ± 0.08	1.398ValMet: 1.398 ± 0.029	1.571ValAsn: 1.571 ± 0.028	5.302ValPro: 5.302 ± 0.051	2.049ValGln: 2.049 ± 0.03	7.17ValArg: 7.17 ± 0.057	4.313ValSer: 4.313 ± 0.049	5.524ValThr: 5.524 ± 0.06	7.635ValVal: 7.635 ± 0.067	1.083ValTrp: 1.083 ± 0.021	1.504ValTyr: 1.504 ± 0.023	0.0ValXaa: 0.0 ± 0.0
Trp	1.62TrpAla: 1.62 ± 0.028	0.155TrpCys: 0.155 ± 0.007	0.792TrpAsp: 0.792 ± 0.019	0.747TrpGlu: 0.747 ± 0.018	0.501TrpPhe: 0.501 ± 0.016	1.018TrpGly: 1.018 ± 0.023	0.347TrpHis: 0.347 ± 0.012	0.567TrpIle: 0.567 ± 0.014	0.388TrpLys: 0.388 ± 0.014	1.652TrpLeu: 1.652 ± 0.027	0.29TrpMet: 0.29 ± 0.012	0.384TrpAsn: 0.384 ± 0.013	0.777TrpPro: 0.777 ± 0.019	0.607TrpGln: 0.607 ± 0.016	1.288TrpArg: 1.288 ± 0.026	0.932TrpSer: 0.932 ± 0.016	1.007TrpThr: 1.007 ± 0.022	0.964TrpVal: 0.964 ± 0.022	0.322TrpTrp: 0.322 ± 0.013	0.343TrpTyr: 0.343 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.826TyrAla: 2.826 ± 0.033	0.164TyrCys: 0.164 ± 0.008	1.425TyrAsp: 1.425 ± 0.031	1.277TyrGlu: 1.277 ± 0.025	0.622TyrPhe: 0.622 ± 0.017	2.277TyrGly: 2.277 ± 0.037	0.369TyrHis: 0.369 ± 0.012	0.497TyrIle: 0.497 ± 0.013	0.38TyrLys: 0.38 ± 0.014	2.06TyrLeu: 2.06 ± 0.031	0.235TyrMet: 0.235 ± 0.011	0.379TyrAsn: 0.379 ± 0.014	1.042TyrPro: 1.042 ± 0.026	0.545TyrGln: 0.545 ± 0.016	1.852TyrArg: 1.852 ± 0.028	0.899TyrSer: 0.899 ± 0.021	1.153TyrThr: 1.153 ± 0.023	1.611TyrVal: 1.611 ± 0.024	0.349TyrTrp: 0.349 ± 0.013	0.413TyrTyr: 0.413 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.006XaaXaa: 0.006 ± 0.005

Statistics based on 7035 proteins (2387058 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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