Enterococcus rivorum
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3427 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E5KZ37|A0A1E5KZ37_9ENTE Uncharacterized protein OS=Enterococcus rivorum OX=762845 GN=BCR26_02125 PE=4 SV=1
MM1 pKa = 7.16 QQFLEE6 pKa = 4.2 NKK8 pKa = 9.36 QEE10 pKa = 4.06 EE11 pKa = 4.47 FHH13 pKa = 6.72 KK14 pKa = 10.68 QLNDD18 pKa = 4.78 GISEE22 pKa = 4.17 FLLEE26 pKa = 4.68 QMDD29 pKa = 3.63 EE30 pKa = 4.2 TFYY33 pKa = 11.71 AFALDD38 pKa = 3.86 CNIYY42 pKa = 9.91 EE43 pKa = 3.95 QGEE46 pKa = 4.29 INFCFNTIDD55 pKa = 3.27 EE56 pKa = 4.4 WEE58 pKa = 4.21 KK59 pKa = 9.6 TKK61 pKa = 10.39 IRR63 pKa = 11.84 YY64 pKa = 9.02 GEE66 pKa = 4.08 YY67 pKa = 10.37 NEE69 pKa = 5.01 EE70 pKa = 3.89 EE71 pKa = 4.18 LLEE74 pKa = 4.14 TKK76 pKa = 10.64 YY77 pKa = 11.34 NSGDD81 pKa = 3.13 WEE83 pKa = 4.44 KK84 pKa = 10.88 YY85 pKa = 8.7 QGFEE89 pKa = 4.27 TIYY92 pKa = 11.08 LFDD95 pKa = 4.15 DD96 pKa = 3.41 WVEE99 pKa = 3.94 EE100 pKa = 4.23 EE101 pKa = 5.12 EE102 pKa = 5.46 DD103 pKa = 5.15 IEE105 pKa = 4.96 YY106 pKa = 10.56 CLNWLCEE113 pKa = 3.86 QMILFLDD120 pKa = 3.83 TSIFQAIPKK129 pKa = 9.22 TEE131 pKa = 3.95 DD132 pKa = 3.17 FKK134 pKa = 11.47 ILVYY138 pKa = 10.97 DD139 pKa = 3.83 HH140 pKa = 7.21 DD141 pKa = 4.45 TDD143 pKa = 3.79 VTSSQEE149 pKa = 3.87 RR150 pKa = 11.84 FEE152 pKa = 4.78 DD153 pKa = 4.29 FSDD156 pKa = 3.71 IEE158 pKa = 4.11 LFKK161 pKa = 10.93 IKK163 pKa = 10.45 SS164 pKa = 3.39
Molecular weight: 19.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.787
IPC2_protein 3.948
IPC_protein 3.897
Toseland 3.719
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.732
Rodwell 3.732
Grimsley 3.63
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.101
Thurlkill 3.745
EMBOSS 3.745
Sillero 4.012
Patrickios 1.875
IPC_peptide 3.846
IPC2_peptide 3.999
IPC2.peptide.svr19 3.897
Protein with the highest isoelectric point:
>tr|A0A1E5KZ59|A0A1E5KZ59_9ENTE Peptide ABC transporter ATP-binding protein OS=Enterococcus rivorum OX=762845 GN=BCR26_01815 PE=3 SV=1
MM1 pKa = 7.92 PKK3 pKa = 10.12 QKK5 pKa = 7.54 THH7 pKa = 6.73 RR8 pKa = 11.84 GSAKK12 pKa = 9.62 RR13 pKa = 11.84 FKK15 pKa = 10.04 RR16 pKa = 11.84 TGKK19 pKa = 10.53 GGLKK23 pKa = 9.97 RR24 pKa = 11.84 FRR26 pKa = 11.84 AFTSHH31 pKa = 6.94 RR32 pKa = 11.84 FHH34 pKa = 7.48 GKK36 pKa = 6.37 TKK38 pKa = 9.57 KK39 pKa = 9.43 QRR41 pKa = 11.84 RR42 pKa = 11.84 QLRR45 pKa = 11.84 KK46 pKa = 9.82 ASMVSAGDD54 pKa = 3.61 FKK56 pKa = 11.18 RR57 pKa = 11.84 IRR59 pKa = 11.84 QQLARR64 pKa = 11.84 MKK66 pKa = 10.67
Molecular weight: 7.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 10.979
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.544
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.266
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.04
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3427
0
3427
1049214
29
2523
306.2
34.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.525 ± 0.043
0.693 ± 0.013
5.013 ± 0.03
7.662 ± 0.051
4.682 ± 0.038
6.46 ± 0.041
1.656 ± 0.018
7.963 ± 0.036
7.65 ± 0.04
9.783 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.636 ± 0.02
5.024 ± 0.033
3.257 ± 0.025
3.97 ± 0.026
3.486 ± 0.029
6.3 ± 0.037
5.827 ± 0.04
6.779 ± 0.037
0.864 ± 0.014
3.769 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here