candidate division SR1 bacterium RAAC1_SR1_1

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Candidatus Absconditabacteria; unclassified Candidatus Absconditabacteria

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1059 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A288DD64|A0A288DD64_9BACT Uncharacterized protein OS=candidate division SR1 bacterium RAAC1_SR1_1 OX=1394709 GN=P148_SR1C00001G0750 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.22KK3 pKa = 9.8ILIAALFILGIFASFTRR20 pKa = 11.84AEE22 pKa = 4.28EE23 pKa = 4.18LNSGNVISTFNCEE36 pKa = 3.81TGATSCDD43 pKa = 3.14LFGRR47 pKa = 11.84NITSIASDD55 pKa = 3.34SFINHH60 pKa = 6.28SNLQYY65 pKa = 11.01LAIRR69 pKa = 11.84NNPLTGIDD77 pKa = 3.24VGDD80 pKa = 4.01FVGLDD85 pKa = 3.65NLIGLYY91 pKa = 9.39MDD93 pKa = 4.87GGNLTSIEE101 pKa = 4.25SGSFLGLSNLNIIDD115 pKa = 4.56LDD117 pKa = 4.01QNKK120 pKa = 8.16LTTIKK125 pKa = 10.75AGTFNGLSSLIRR137 pKa = 11.84LEE139 pKa = 3.95ISQNQITSIEE149 pKa = 4.19SGGFLGATNLTGLLLDD165 pKa = 4.3NNLITGIDD173 pKa = 3.35VGDD176 pKa = 4.46FIGLTNLDD184 pKa = 4.29YY185 pKa = 11.47LSLTQNQISSIEE197 pKa = 4.03RR198 pKa = 11.84GSFSGLFLLKK208 pKa = 10.41NLYY211 pKa = 10.63LNYY214 pKa = 10.56NQITSIDD221 pKa = 3.54VGDD224 pKa = 5.56FIGLDD229 pKa = 3.24NLTQLNLNINKK240 pKa = 9.41ISSIEE245 pKa = 3.89AYY247 pKa = 10.05GFSEE251 pKa = 4.7LSSLTYY257 pKa = 11.04LNLGYY262 pKa = 10.39NQINSIEE269 pKa = 4.21SGSFYY274 pKa = 11.15GLDD277 pKa = 3.55NLTDD281 pKa = 4.37LYY283 pKa = 11.18LGNFKK288 pKa = 10.56LPRR291 pKa = 11.84DD292 pKa = 3.69IGPEE296 pKa = 3.72NVVYY300 pKa = 10.61NQISSIDD307 pKa = 3.54VGDD310 pKa = 3.75FAGLNNLTQLSLDD323 pKa = 3.6GNAISSIEE331 pKa = 4.06SGSFIGLSFLTYY343 pKa = 10.75LDD345 pKa = 4.58LNNNQIDD352 pKa = 4.34SIDD355 pKa = 3.95LGDD358 pKa = 4.11FADD361 pKa = 5.03LSSLTDD367 pKa = 3.95LYY369 pKa = 11.34LYY371 pKa = 10.86GNEE374 pKa = 3.66ISFIEE379 pKa = 4.37SNSFNLLSSLTYY391 pKa = 10.66LDD393 pKa = 4.97LGNNQINSIDD403 pKa = 3.23IGDD406 pKa = 3.95FVGLTNLTDD415 pKa = 4.14LYY417 pKa = 11.03LYY419 pKa = 10.75GNEE422 pKa = 3.87ISSIGSNSFNLLSSLTYY439 pKa = 10.73LDD441 pKa = 5.17LNSNQINSIEE451 pKa = 4.15SGDD454 pKa = 3.9FNGLSSLIFLDD465 pKa = 5.3LSTNQINSIEE475 pKa = 4.19SGDD478 pKa = 3.9FNGLSSLNEE487 pKa = 4.07LYY489 pKa = 10.88LSYY492 pKa = 11.11NQINSIEE499 pKa = 4.01VSGFSGLPSLMYY511 pKa = 10.57LYY513 pKa = 10.87LDD515 pKa = 3.75NNQLLSIDD523 pKa = 3.38VGNFYY528 pKa = 10.86EE529 pKa = 4.9LDD531 pKa = 3.72NLNGLSLNKK540 pKa = 10.19NFISSITSNVFDD552 pKa = 4.5NLLNLWDD559 pKa = 4.83LSLSSICTDD568 pKa = 3.0NPGIIAYY575 pKa = 8.88PNGSTEE581 pKa = 4.18TNNNYY586 pKa = 10.05CSVINSTNQIDD597 pKa = 5.41FITEE601 pKa = 3.79LTKK604 pKa = 10.84EE605 pKa = 4.18VMDD608 pKa = 4.72GKK610 pKa = 9.33ITLALYY616 pKa = 10.32SLSGNYY622 pKa = 7.46YY623 pKa = 7.88TGTYY627 pKa = 9.82AISFTPGITNEE638 pKa = 3.97ILVDD642 pKa = 4.61IIMQSPTSNYY652 pKa = 8.05TQDD655 pKa = 3.52YY656 pKa = 8.3YY657 pKa = 11.78LNNVEE662 pKa = 4.11VQYY665 pKa = 11.58DD666 pKa = 3.79SGTLLTTNGQGFTGILQAPVLLSTGSTKK694 pKa = 10.75LVDD697 pKa = 3.46NVVALTKK704 pKa = 10.84FGDD707 pKa = 3.25ATKK710 pKa = 10.49RR711 pKa = 11.84IDD713 pKa = 3.51FSKK716 pKa = 10.63SVTVRR721 pKa = 11.84MPALGKK727 pKa = 10.5NIDD730 pKa = 3.9DD731 pKa = 4.08VVDD734 pKa = 3.74IFYY737 pKa = 10.98SQNLSSNWSFHH748 pKa = 4.98GTSTVIDD755 pKa = 3.59IAGIPYY761 pKa = 9.83VEE763 pKa = 4.69FTTDD767 pKa = 2.61HH768 pKa = 5.96ATYY771 pKa = 9.83FAIGEE776 pKa = 4.47GTGSFVINNDD786 pKa = 3.62DD787 pKa = 4.75ASTNSQNVTLNISGAIAYY805 pKa = 7.51MRR807 pKa = 11.84FSNDD811 pKa = 2.57NSNRR815 pKa = 11.84SAWEE819 pKa = 4.17SISDD823 pKa = 3.71TKK825 pKa = 11.29SRR827 pKa = 11.84TLSAGTGTKK836 pKa = 8.43TVYY839 pKa = 10.95AEE841 pKa = 4.31FDD843 pKa = 3.66LDD845 pKa = 3.67SDD847 pKa = 4.62FIADD851 pKa = 3.73ISTSDD856 pKa = 3.52TIEE859 pKa = 4.05YY860 pKa = 10.13TDD862 pKa = 3.87GSSGNGTNGNITLTITGGVTEE883 pKa = 5.18CIYY886 pKa = 10.08GTSLTMDD893 pKa = 3.43AQEE896 pKa = 4.24VKK898 pKa = 10.45IGVPYY903 pKa = 10.3TFTGTFPSTWYY914 pKa = 9.58CQDD917 pKa = 2.89YY918 pKa = 10.97RR919 pKa = 11.84GINGGWTLTIQTTDD933 pKa = 4.5LINEE937 pKa = 4.13KK938 pKa = 10.79SNVISGSNLLISHH951 pKa = 6.44NPVVVEE957 pKa = 4.31GDD959 pKa = 3.86SACTGDD965 pKa = 5.24NGTATQFYY973 pKa = 8.85SAPYY977 pKa = 9.63EE978 pKa = 4.08IFEE981 pKa = 5.7KK982 pKa = 10.63IPDD985 pKa = 3.65SNKK988 pKa = 8.87ICRR991 pKa = 11.84VSADD995 pKa = 3.49NVGLLVNVPANQAPGSYY1012 pKa = 10.0SGTLTLTMNGFF1023 pKa = 3.76

Molecular weight: 110.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A288DJ48|A0A288DJ48_9BACT SsrA-binding protein OS=candidate division SR1 bacterium RAAC1_SR1_1 OX=1394709 GN=smpB PE=3 SV=1
MM1 pKa = 7.46ASKK4 pKa = 10.42LRR6 pKa = 11.84RR7 pKa = 11.84VRR9 pKa = 11.84KK10 pKa = 7.87YY11 pKa = 10.4SRR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 9.7RR16 pKa = 11.84LTKK19 pKa = 10.01HH20 pKa = 5.54GFRR23 pKa = 11.84EE24 pKa = 3.94RR25 pKa = 11.84LQTKK29 pKa = 9.65NGKK32 pKa = 9.16KK33 pKa = 9.82LLARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84AKK41 pKa = 10.06GRR43 pKa = 11.84KK44 pKa = 8.83SLTVQRR50 pKa = 11.84KK51 pKa = 7.82KK52 pKa = 11.02

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1059

0

1059

355954

29

5926

336.1

37.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.092 ± 0.066

0.936 ± 0.038

5.48 ± 0.064

6.591 ± 0.117

5.014 ± 0.068

6.765 ± 0.102

1.595 ± 0.048

9.289 ± 0.088

8.726 ± 0.164

9.073 ± 0.095

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.049

5.869 ± 0.147

3.156 ± 0.049

3.99 ± 0.052

3.194 ± 0.059

6.393 ± 0.122

6.294 ± 0.173

5.535 ± 0.075

0.705 ± 0.019

4.119 ± 0.063
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski