Amino acid dipepetide frequency for Pseudoalteromonas phage SL20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.53AlaAla: 4.53 ± 0.811
0.576AlaCys: 0.576 ± 0.137
3.294AlaAsp: 3.294 ± 0.394
3.871AlaGlu: 3.871 ± 0.37
2.018AlaPhe: 2.018 ± 0.261
3.747AlaGly: 3.747 ± 0.492
1.029AlaHis: 1.029 ± 0.195
3.541AlaIle: 3.541 ± 0.509
4.941AlaLys: 4.941 ± 0.517
4.282AlaLeu: 4.282 ± 0.565
0.988AlaMet: 0.988 ± 0.232
2.841AlaAsn: 2.841 ± 0.4
1.441AlaPro: 1.441 ± 0.244
2.059AlaGln: 2.059 ± 0.264
1.977AlaArg: 1.977 ± 0.298
4.282AlaSer: 4.282 ± 0.47
4.612AlaThr: 4.612 ± 0.389
3.088AlaVal: 3.088 ± 0.38
0.782AlaTrp: 0.782 ± 0.162
2.8AlaTyr: 2.8 ± 0.363
0.0AlaXaa: 0.0 ± 0.0
Cys
0.453CysAla: 0.453 ± 0.141
0.535CysCys: 0.535 ± 0.159
0.906CysAsp: 0.906 ± 0.187
0.906CysGlu: 0.906 ± 0.179
1.194CysPhe: 1.194 ± 0.275
1.071CysGly: 1.071 ± 0.268
0.494CysHis: 0.494 ± 0.134
0.741CysIle: 0.741 ± 0.156
1.194CysLys: 1.194 ± 0.253
1.112CysLeu: 1.112 ± 0.193
0.288CysMet: 0.288 ± 0.111
1.194CysAsn: 1.194 ± 0.229
0.576CysPro: 0.576 ± 0.163
0.288CysGln: 0.288 ± 0.087
0.576CysArg: 0.576 ± 0.171
1.112CysSer: 1.112 ± 0.239
0.947CysThr: 0.947 ± 0.193
1.153CysVal: 1.153 ± 0.204
0.206CysTrp: 0.206 ± 0.094
0.782CysTyr: 0.782 ± 0.176
0.0CysXaa: 0.0 ± 0.0
Asp
3.294AspAla: 3.294 ± 0.418
1.029AspCys: 1.029 ± 0.213
3.706AspAsp: 3.706 ± 0.309
4.735AspGlu: 4.735 ± 0.518
2.924AspPhe: 2.924 ± 0.45
4.282AspGly: 4.282 ± 0.415
1.029AspHis: 1.029 ± 0.215
4.447AspIle: 4.447 ± 0.452
6.588AspLys: 6.588 ± 0.572
5.6AspLeu: 5.6 ± 0.44
1.977AspMet: 1.977 ± 0.27
4.818AspAsn: 4.818 ± 0.492
1.524AspPro: 1.524 ± 0.226
1.565AspGln: 1.565 ± 0.3
2.347AspArg: 2.347 ± 0.369
4.818AspSer: 4.818 ± 0.546
3.5AspThr: 3.5 ± 0.379
4.365AspVal: 4.365 ± 0.432
1.112AspTrp: 1.112 ± 0.233
3.871AspTyr: 3.871 ± 0.443
0.0AspXaa: 0.0 ± 0.0
Glu
4.365GluAla: 4.365 ± 0.456
0.782GluCys: 0.782 ± 0.194
6.424GluAsp: 6.424 ± 0.549
6.712GluGlu: 6.712 ± 0.605
3.047GluPhe: 3.047 ± 0.304
5.847GluGly: 5.847 ± 0.482
0.988GluHis: 0.988 ± 0.239
4.612GluIle: 4.612 ± 0.523
5.188GluLys: 5.188 ± 0.543
6.094GluLeu: 6.094 ± 0.566
1.729GluMet: 1.729 ± 0.296
3.83GluAsn: 3.83 ± 0.37
1.235GluPro: 1.235 ± 0.189
2.059GluGln: 2.059 ± 0.322
2.388GluArg: 2.388 ± 0.283
4.777GluSer: 4.777 ± 0.425
3.13GluThr: 3.13 ± 0.35
5.847GluVal: 5.847 ± 0.497
1.029GluTrp: 1.029 ± 0.22
3.582GluTyr: 3.582 ± 0.423
0.0GluXaa: 0.0 ± 0.0
Phe
2.306PheAla: 2.306 ± 0.288
0.618PheCys: 0.618 ± 0.188
2.635PheAsp: 2.635 ± 0.337
3.377PheGlu: 3.377 ± 0.318
0.947PhePhe: 0.947 ± 0.203
2.594PheGly: 2.594 ± 0.387
0.947PheHis: 0.947 ± 0.181
2.635PheIle: 2.635 ± 0.298
4.324PheLys: 4.324 ± 0.472
3.253PheLeu: 3.253 ± 0.417
1.235PheMet: 1.235 ± 0.241
3.13PheAsn: 3.13 ± 0.341
1.235PhePro: 1.235 ± 0.205
1.482PheGln: 1.482 ± 0.252
1.647PheArg: 1.647 ± 0.249
2.1PheSer: 2.1 ± 0.256
3.212PheThr: 3.212 ± 0.385
2.553PheVal: 2.553 ± 0.294
0.412PheTrp: 0.412 ± 0.133
2.141PheTyr: 2.141 ± 0.316
0.0PheXaa: 0.0 ± 0.0
Gly
3.788GlyAla: 3.788 ± 0.507
1.565GlyCys: 1.565 ± 0.265
4.282GlyAsp: 4.282 ± 0.406
4.447GlyGlu: 4.447 ± 0.427
3.294GlyPhe: 3.294 ± 0.316
5.765GlyGly: 5.765 ± 0.593
1.029GlyHis: 1.029 ± 0.219
3.582GlyIle: 3.582 ± 0.373
6.341GlyLys: 6.341 ± 0.463
4.9GlyLeu: 4.9 ± 0.479
1.647GlyMet: 1.647 ± 0.276
4.035GlyAsn: 4.035 ± 0.421
0.206GlyPro: 0.206 ± 0.091
1.729GlyGln: 1.729 ± 0.299
2.882GlyArg: 2.882 ± 0.337
5.147GlySer: 5.147 ± 0.458
3.582GlyThr: 3.582 ± 0.441
5.435GlyVal: 5.435 ± 0.447
0.865GlyTrp: 0.865 ± 0.201
3.335GlyTyr: 3.335 ± 0.397
0.0GlyXaa: 0.0 ± 0.0
His
0.7HisAla: 0.7 ± 0.18
0.412HisCys: 0.412 ± 0.131
0.906HisAsp: 0.906 ± 0.179
0.741HisGlu: 0.741 ± 0.159
1.071HisPhe: 1.071 ± 0.194
0.988HisGly: 0.988 ± 0.189
0.494HisHis: 0.494 ± 0.136
1.194HisIle: 1.194 ± 0.229
1.194HisLys: 1.194 ± 0.223
1.729HisLeu: 1.729 ± 0.257
0.659HisMet: 0.659 ± 0.15
1.153HisAsn: 1.153 ± 0.256
0.741HisPro: 0.741 ± 0.161
0.576HisGln: 0.576 ± 0.162
0.906HisArg: 0.906 ± 0.203
1.359HisSer: 1.359 ± 0.283
1.235HisThr: 1.235 ± 0.249
0.659HisVal: 0.659 ± 0.153
0.165HisTrp: 0.165 ± 0.076
0.782HisTyr: 0.782 ± 0.167
0.0HisXaa: 0.0 ± 0.0
Ile
4.035IleAla: 4.035 ± 0.332
0.7IleCys: 0.7 ± 0.194
4.941IleAsp: 4.941 ± 0.525
4.447IleGlu: 4.447 ± 0.426
2.1IlePhe: 2.1 ± 0.268
3.377IleGly: 3.377 ± 0.436
1.112IleHis: 1.112 ± 0.179
3.541IleIle: 3.541 ± 0.398
4.859IleLys: 4.859 ± 0.414
4.571IleLeu: 4.571 ± 0.453
1.194IleMet: 1.194 ± 0.216
3.788IleAsn: 3.788 ± 0.485
2.553IlePro: 2.553 ± 0.313
1.606IleGln: 1.606 ± 0.276
2.388IleArg: 2.388 ± 0.29
4.241IleSer: 4.241 ± 0.456
4.612IleThr: 4.612 ± 0.514
3.541IleVal: 3.541 ± 0.402
0.288IleTrp: 0.288 ± 0.093
1.894IleTyr: 1.894 ± 0.272
0.0IleXaa: 0.0 ± 0.0
Lys
4.2LysAla: 4.2 ± 0.423
1.235LysCys: 1.235 ± 0.222
6.094LysAsp: 6.094 ± 0.607
7.083LysGlu: 7.083 ± 0.705
3.13LysPhe: 3.13 ± 0.338
5.641LysGly: 5.641 ± 0.529
1.441LysHis: 1.441 ± 0.282
4.2LysIle: 4.2 ± 0.436
6.177LysLys: 6.177 ± 0.701
6.424LysLeu: 6.424 ± 0.538
1.935LysMet: 1.935 ± 0.355
4.077LysAsn: 4.077 ± 0.434
2.141LysPro: 2.141 ± 0.343
2.347LysGln: 2.347 ± 0.321
3.871LysArg: 3.871 ± 0.524
5.641LysSer: 5.641 ± 0.492
4.447LysThr: 4.447 ± 0.43
6.3LysVal: 6.3 ± 0.512
1.029LysTrp: 1.029 ± 0.205
3.541LysTyr: 3.541 ± 0.375
0.0LysXaa: 0.0 ± 0.0
Leu
5.188LeuAla: 5.188 ± 0.543
1.194LeuCys: 1.194 ± 0.245
5.394LeuAsp: 5.394 ± 0.484
6.465LeuGlu: 6.465 ± 0.649
3.377LeuPhe: 3.377 ± 0.42
5.065LeuGly: 5.065 ± 0.464
1.606LeuHis: 1.606 ± 0.263
4.035LeuIle: 4.035 ± 0.478
5.559LeuLys: 5.559 ± 0.601
6.918LeuLeu: 6.918 ± 0.588
2.182LeuMet: 2.182 ± 0.279
4.118LeuAsn: 4.118 ± 0.526
4.159LeuPro: 4.159 ± 0.377
2.965LeuGln: 2.965 ± 0.337
3.13LeuArg: 3.13 ± 0.322
6.506LeuSer: 6.506 ± 0.537
5.106LeuThr: 5.106 ± 0.471
5.147LeuVal: 5.147 ± 0.488
0.659LeuTrp: 0.659 ± 0.189
2.471LeuTyr: 2.471 ± 0.321
0.0LeuXaa: 0.0 ± 0.0
Met
1.894MetAla: 1.894 ± 0.293
0.329MetCys: 0.329 ± 0.125
1.4MetAsp: 1.4 ± 0.261
1.729MetGlu: 1.729 ± 0.309
1.524MetPhe: 1.524 ± 0.222
1.318MetGly: 1.318 ± 0.223
0.412MetHis: 0.412 ± 0.12
1.771MetIle: 1.771 ± 0.234
1.771MetLys: 1.771 ± 0.301
1.647MetLeu: 1.647 ± 0.285
0.453MetMet: 0.453 ± 0.155
0.947MetAsn: 0.947 ± 0.17
0.618MetPro: 0.618 ± 0.176
0.7MetGln: 0.7 ± 0.185
0.782MetArg: 0.782 ± 0.152
1.729MetSer: 1.729 ± 0.247
1.359MetThr: 1.359 ± 0.244
1.235MetVal: 1.235 ± 0.283
0.165MetTrp: 0.165 ± 0.075
0.7MetTyr: 0.7 ± 0.179
0.0MetXaa: 0.0 ± 0.0
Asn
3.171AsnAla: 3.171 ± 0.402
0.865AsnCys: 0.865 ± 0.277
2.553AsnAsp: 2.553 ± 0.247
3.047AsnGlu: 3.047 ± 0.303
2.594AsnPhe: 2.594 ± 0.295
4.324AsnGly: 4.324 ± 0.52
0.824AsnHis: 0.824 ± 0.217
4.159AsnIle: 4.159 ± 0.607
4.9AsnLys: 4.9 ± 0.485
4.488AsnLeu: 4.488 ± 0.385
1.482AsnMet: 1.482 ± 0.281
3.994AsnAsn: 3.994 ± 0.446
2.306AsnPro: 2.306 ± 0.273
1.565AsnGln: 1.565 ± 0.243
2.1AsnArg: 2.1 ± 0.28
3.377AsnSer: 3.377 ± 0.422
3.912AsnThr: 3.912 ± 0.399
3.706AsnVal: 3.706 ± 0.448
0.741AsnTrp: 0.741 ± 0.183
3.047AsnTyr: 3.047 ± 0.357
0.0AsnXaa: 0.0 ± 0.0
Pro
1.771ProAla: 1.771 ± 0.268
0.618ProCys: 0.618 ± 0.151
2.677ProAsp: 2.677 ± 0.387
2.718ProGlu: 2.718 ± 0.338
1.977ProPhe: 1.977 ± 0.242
0.0ProGly: 0.0 ± 0.0
0.371ProHis: 0.371 ± 0.122
1.894ProIle: 1.894 ± 0.274
2.429ProLys: 2.429 ± 0.365
2.347ProLeu: 2.347 ± 0.366
0.247ProMet: 0.247 ± 0.091
1.977ProAsn: 1.977 ± 0.324
1.112ProPro: 1.112 ± 0.227
1.153ProGln: 1.153 ± 0.215
1.071ProArg: 1.071 ± 0.23
2.759ProSer: 2.759 ± 0.362
2.141ProThr: 2.141 ± 0.266
1.977ProVal: 1.977 ± 0.297
0.494ProTrp: 0.494 ± 0.129
1.482ProTyr: 1.482 ± 0.203
0.0ProXaa: 0.0 ± 0.0
Gln
2.265GlnAla: 2.265 ± 0.34
0.453GlnCys: 0.453 ± 0.124
2.1GlnAsp: 2.1 ± 0.283
2.429GlnGlu: 2.429 ± 0.358
0.947GlnPhe: 0.947 ± 0.186
2.182GlnGly: 2.182 ± 0.264
0.7GlnHis: 0.7 ± 0.176
1.565GlnIle: 1.565 ± 0.234
2.347GlnLys: 2.347 ± 0.417
2.8GlnLeu: 2.8 ± 0.488
0.906GlnMet: 0.906 ± 0.178
1.153GlnAsn: 1.153 ± 0.246
0.659GlnPro: 0.659 ± 0.226
1.071GlnGln: 1.071 ± 0.19
1.647GlnArg: 1.647 ± 0.262
2.141GlnSer: 2.141 ± 0.335
1.441GlnThr: 1.441 ± 0.307
2.141GlnVal: 2.141 ± 0.323
0.618GlnTrp: 0.618 ± 0.14
1.318GlnTyr: 1.318 ± 0.242
0.0GlnXaa: 0.0 ± 0.0
Arg
1.894ArgAla: 1.894 ± 0.305
0.741ArgCys: 0.741 ± 0.195
1.771ArgAsp: 1.771 ± 0.275
3.377ArgGlu: 3.377 ± 0.438
1.688ArgPhe: 1.688 ± 0.242
2.677ArgGly: 2.677 ± 0.338
0.453ArgHis: 0.453 ± 0.132
2.059ArgIle: 2.059 ± 0.273
4.077ArgLys: 4.077 ± 0.457
3.088ArgLeu: 3.088 ± 0.293
0.947ArgMet: 0.947 ± 0.184
2.182ArgAsn: 2.182 ± 0.267
1.153ArgPro: 1.153 ± 0.21
1.277ArgGln: 1.277 ± 0.229
1.688ArgArg: 1.688 ± 0.339
3.088ArgSer: 3.088 ± 0.379
1.771ArgThr: 1.771 ± 0.263
2.8ArgVal: 2.8 ± 0.298
0.412ArgTrp: 0.412 ± 0.123
1.688ArgTyr: 1.688 ± 0.276
0.0ArgXaa: 0.0 ± 0.0
Ser
3.994SerAla: 3.994 ± 0.474
1.359SerCys: 1.359 ± 0.316
5.353SerAsp: 5.353 ± 0.455
4.982SerGlu: 4.982 ± 0.485
2.965SerPhe: 2.965 ± 0.301
6.465SerGly: 6.465 ± 0.641
1.194SerHis: 1.194 ± 0.252
4.035SerIle: 4.035 ± 0.472
4.488SerLys: 4.488 ± 0.525
6.877SerLeu: 6.877 ± 0.587
1.482SerMet: 1.482 ± 0.29
3.212SerAsn: 3.212 ± 0.407
2.306SerPro: 2.306 ± 0.285
2.059SerGln: 2.059 ± 0.316
2.8SerArg: 2.8 ± 0.326
4.53SerSer: 4.53 ± 0.448
3.582SerThr: 3.582 ± 0.352
4.982SerVal: 4.982 ± 0.501
0.947SerTrp: 0.947 ± 0.164
3.335SerTyr: 3.335 ± 0.35
0.0SerXaa: 0.0 ± 0.0
Thr
2.924ThrAla: 2.924 ± 0.396
0.659ThrCys: 0.659 ± 0.153
3.788ThrAsp: 3.788 ± 0.548
3.665ThrGlu: 3.665 ± 0.418
2.677ThrPhe: 2.677 ± 0.322
4.735ThrGly: 4.735 ± 0.49
1.153ThrHis: 1.153 ± 0.225
4.118ThrIle: 4.118 ± 0.369
4.447ThrLys: 4.447 ± 0.365
5.394ThrLeu: 5.394 ± 0.48
0.7ThrMet: 0.7 ± 0.162
2.882ThrAsn: 2.882 ± 0.312
2.759ThrPro: 2.759 ± 0.45
1.935ThrGln: 1.935 ± 0.274
2.306ThrArg: 2.306 ± 0.344
4.118ThrSer: 4.118 ± 0.449
3.788ThrThr: 3.788 ± 0.456
3.788ThrVal: 3.788 ± 0.431
1.029ThrTrp: 1.029 ± 0.209
2.677ThrTyr: 2.677 ± 0.371
0.0ThrXaa: 0.0 ± 0.0
Val
3.5ValAla: 3.5 ± 0.44
1.029ValCys: 1.029 ± 0.196
5.23ValAsp: 5.23 ± 0.519
5.106ValGlu: 5.106 ± 0.52
2.471ValPhe: 2.471 ± 0.319
4.653ValGly: 4.653 ± 0.465
1.359ValHis: 1.359 ± 0.251
4.488ValIle: 4.488 ± 0.369
4.612ValLys: 4.612 ± 0.449
4.941ValLeu: 4.941 ± 0.454
1.153ValMet: 1.153 ± 0.207
4.406ValAsn: 4.406 ± 0.43
2.429ValPro: 2.429 ± 0.424
2.471ValGln: 2.471 ± 0.303
2.059ValArg: 2.059 ± 0.272
4.735ValSer: 4.735 ± 0.526
3.706ValThr: 3.706 ± 0.54
4.406ValVal: 4.406 ± 0.446
1.112ValTrp: 1.112 ± 0.245
2.759ValTyr: 2.759 ± 0.379
0.0ValXaa: 0.0 ± 0.0
Trp
0.535TrpAla: 0.535 ± 0.138
0.371TrpCys: 0.371 ± 0.157
0.865TrpAsp: 0.865 ± 0.19
1.153TrpGlu: 1.153 ± 0.228
0.659TrpPhe: 0.659 ± 0.144
0.782TrpGly: 0.782 ± 0.188
0.247TrpHis: 0.247 ± 0.098
0.782TrpIle: 0.782 ± 0.194
1.071TrpLys: 1.071 ± 0.252
1.071TrpLeu: 1.071 ± 0.189
0.494TrpMet: 0.494 ± 0.148
0.659TrpAsn: 0.659 ± 0.16
0.165TrpPro: 0.165 ± 0.079
0.329TrpGln: 0.329 ± 0.104
0.371TrpArg: 0.371 ± 0.123
0.659TrpSer: 0.659 ± 0.142
0.453TrpThr: 0.453 ± 0.111
1.194TrpVal: 1.194 ± 0.208
0.329TrpTrp: 0.329 ± 0.119
0.7TrpTyr: 0.7 ± 0.176
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.935TyrAla: 1.935 ± 0.272
0.659TyrCys: 0.659 ± 0.152
3.212TyrAsp: 3.212 ± 0.357
2.677TyrGlu: 2.677 ± 0.291
2.182TyrPhe: 2.182 ± 0.317
2.347TyrGly: 2.347 ± 0.314
0.782TyrHis: 0.782 ± 0.163
2.388TyrIle: 2.388 ± 0.337
4.447TyrLys: 4.447 ± 0.478
3.706TyrLeu: 3.706 ± 0.379
0.741TyrMet: 0.741 ± 0.165
2.759TyrAsn: 2.759 ± 0.365
1.894TyrPro: 1.894 ± 0.258
1.647TyrGln: 1.647 ± 0.289
1.894TyrArg: 1.894 ± 0.269
3.83TyrSer: 3.83 ± 0.347
3.006TyrThr: 3.006 ± 0.359
2.306TyrVal: 2.306 ± 0.385
0.494TyrTrp: 0.494 ± 0.134
2.594TyrTyr: 2.594 ± 0.38
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (24286 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski