Rhodococcus sp. C9-5

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4272 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A541B0K8|A0A541B0K8_9NOCA Carboxylic ester hydrolase OS=Rhodococcus sp. C9-5 OX=2546320 GN=FK531_20615 PE=3 SV=1
MM1 pKa = 7.74TDD3 pKa = 3.82HH4 pKa = 7.52DD5 pKa = 4.34TTDD8 pKa = 3.87DD9 pKa = 3.45QPLRR13 pKa = 11.84IDD15 pKa = 3.46GALAVVTDD23 pKa = 4.51EE24 pKa = 4.94RR25 pKa = 11.84DD26 pKa = 3.3QLHH29 pKa = 6.37ALCVRR34 pKa = 11.84TLAEE38 pKa = 4.39LLGSEE43 pKa = 5.0PDD45 pKa = 3.38TDD47 pKa = 3.76EE48 pKa = 6.32DD49 pKa = 4.04GDD51 pKa = 3.85IAVPVHH57 pKa = 6.2GFAVYY62 pKa = 8.47VTVADD67 pKa = 5.3DD68 pKa = 4.55GPQLHH73 pKa = 6.22VWSSIVTGITDD84 pKa = 3.64RR85 pKa = 11.84ALATTQLVEE94 pKa = 5.57LSEE97 pKa = 3.99EE98 pKa = 4.04WPRR101 pKa = 11.84LRR103 pKa = 11.84FALSEE108 pKa = 4.0EE109 pKa = 4.14HH110 pKa = 6.94LLVSTLLDD118 pKa = 4.2ADD120 pKa = 4.09PFAPQHH126 pKa = 6.09LLNLIDD132 pKa = 4.03EE133 pKa = 4.67VHH135 pKa = 7.1DD136 pKa = 4.01LTHH139 pKa = 7.67DD140 pKa = 4.14LDD142 pKa = 5.82DD143 pKa = 4.99DD144 pKa = 3.84FAARR148 pKa = 11.84FGGTLDD154 pKa = 4.73CDD156 pKa = 4.21ADD158 pKa = 4.08DD159 pKa = 4.86DD160 pKa = 5.08SYY162 pKa = 12.08AGEE165 pKa = 4.69CGGGGCGDD173 pKa = 4.43DD174 pKa = 4.31CACGHH179 pKa = 7.01DD180 pKa = 4.19GEE182 pKa = 5.29HH183 pKa = 6.52VEE185 pKa = 4.44GDD187 pKa = 3.77LGITPATSSS196 pKa = 3.18

Molecular weight: 20.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A541BR53|A0A541BR53_9NOCA IclR-ED domain-containing protein OS=Rhodococcus sp. C9-5 OX=2546320 GN=FK531_01560 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84QKK40 pKa = 10.62GRR42 pKa = 11.84ASLTAA47 pKa = 4.1

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4272

0

4272

1430120

25

14478

334.8

35.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.316 ± 0.054

0.726 ± 0.013

6.193 ± 0.031

5.359 ± 0.033

2.942 ± 0.023

9.275 ± 0.04

2.052 ± 0.018

3.875 ± 0.027

2.108 ± 0.036

10.062 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.924 ± 0.015

2.051 ± 0.022

5.667 ± 0.034

2.78 ± 0.019

7.221 ± 0.043

5.552 ± 0.022

6.291 ± 0.03

9.18 ± 0.038

1.432 ± 0.016

1.991 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski