Sulfitobacter indolifex HEL-45
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4147 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A9E7L9|A9E7L9_9RHOB TRAP dicarboxylate transporter-DctP subunit OS=Sulfitobacter indolifex HEL-45 OX=391624 GN=OIHEL45_15224 PE=3 SV=1
MM1 pKa = 7.35 HH2 pKa = 7.4 FEE4 pKa = 4.07 LSFIGVFLEE13 pKa = 4.64 RR14 pKa = 11.84 IFGGFFSVARR24 pKa = 11.84 RR25 pKa = 11.84 GRR27 pKa = 11.84 GGAALGLRR35 pKa = 11.84 IFCATLLPLVFTTKK49 pKa = 10.54 GAFAQQLILNTVGAPTVVGAGIGEE73 pKa = 4.07 RR74 pKa = 11.84 ALWSNAGTVGGVPVDD89 pKa = 3.83 IVAEE93 pKa = 4.2 MVFASRR99 pKa = 11.84 DD100 pKa = 3.7 HH101 pKa = 7.09 IFTTGNGQIQITSTGQDD118 pKa = 3.14 PHH120 pKa = 8.36 FMDD123 pKa = 5.31 FYY125 pKa = 10.68 LYY127 pKa = 10.63 EE128 pKa = 5.35 AGTHH132 pKa = 6.64 NITTNSGGVPVVADD146 pKa = 3.4 VFFQINDD153 pKa = 2.92 IDD155 pKa = 4.28 GPLNEE160 pKa = 4.01 QVYY163 pKa = 10.92 VNICDD168 pKa = 3.61 GTVEE172 pKa = 3.98 YY173 pKa = 11.29 VRR175 pKa = 11.84 IDD177 pKa = 3.59 RR178 pKa = 11.84 SATTYY183 pKa = 10.0 RR184 pKa = 11.84 GYY186 pKa = 10.31 IEE188 pKa = 5.93 GPDD191 pKa = 3.51 ANLGTEE197 pKa = 4.15 VFYY200 pKa = 11.04 LAGDD204 pKa = 3.81 RR205 pKa = 11.84 GYY207 pKa = 11.44 SNQPVSGLEE216 pKa = 3.33 IFYY219 pKa = 9.75 PQTSTFNFGRR229 pKa = 11.84 TANNGFLVLLSNPTYY244 pKa = 11.1 NEE246 pKa = 3.76 TQTYY250 pKa = 10.71 DD251 pKa = 3.68 LQCGDD256 pKa = 4.15 FKK258 pKa = 11.69 APVLQDD264 pKa = 3.53 DD265 pKa = 4.69 LKK267 pKa = 10.62 EE268 pKa = 4.15 QVLSEE273 pKa = 4.03 PVVLNILFNDD283 pKa = 3.97 SVATEE288 pKa = 4.26 NNNAPANNSGEE299 pKa = 3.89 PSEE302 pKa = 4.26 YY303 pKa = 10.79 AKK305 pKa = 10.57 QAVDD309 pKa = 5.31 LIPPTGALNIVTDD322 pKa = 4.22 SEE324 pKa = 4.23 GHH326 pKa = 5.37 RR327 pKa = 11.84 VGFDD331 pKa = 3.18 VLGEE335 pKa = 4.39 GTWSYY340 pKa = 12.18 DD341 pKa = 3.39 DD342 pKa = 3.85 LTGEE346 pKa = 4.15 LTFTPFAAFFAAPTSINYY364 pKa = 9.73 RR365 pKa = 11.84 YY366 pKa = 9.63 QSPIVLPNEE375 pKa = 4.03 PQTYY379 pKa = 9.6 SAPAQVSIDD388 pKa = 3.71 VGSVGLLKK396 pKa = 10.54 LAQLVDD402 pKa = 3.88 TNLNGYY408 pKa = 9.82 ADD410 pKa = 4.18 AGEE413 pKa = 4.37 TIAYY417 pKa = 9.6 VLTAEE422 pKa = 4.28 NFGNVDD428 pKa = 3.57 LTNVQLAEE436 pKa = 4.18 TQFSGKK442 pKa = 9.74 GVPPVITFQAATSLSPEE459 pKa = 3.84 GTLLVGEE466 pKa = 4.4 KK467 pKa = 10.26 AVYY470 pKa = 7.91 TATYY474 pKa = 9.11 TLVPEE479 pKa = 5.2 DD480 pKa = 4.76 LDD482 pKa = 4.03 TTISNQAEE490 pKa = 4.36 VTAQTPGGTVVSDD503 pKa = 4.49 LSDD506 pKa = 3.56 SEE508 pKa = 4.51 NPGDD512 pKa = 4.87 GDD514 pKa = 3.9 GTPSNGPGAGRR525 pKa = 11.84 DD526 pKa = 3.85 DD527 pKa = 3.86 PTTIYY532 pKa = 10.61 AGSGPDD538 pKa = 4.01 RR539 pKa = 11.84 GDD541 pKa = 3.35 APITYY546 pKa = 10.05 GDD548 pKa = 4.06 PQHH551 pKa = 7.13 ADD553 pKa = 2.92 TSQYY557 pKa = 10.25 WIGALNGDD565 pKa = 3.97 GDD567 pKa = 4.06 GSAQHH572 pKa = 6.5 SVDD575 pKa = 3.33 GTGDD579 pKa = 3.8 DD580 pKa = 5.05 LDD582 pKa = 5.42 GNDD585 pKa = 5.05 DD586 pKa = 3.68 EE587 pKa = 5.95 SEE589 pKa = 4.01 EE590 pKa = 4.82 AFPQLYY596 pKa = 10.93 GDD598 pKa = 4.44 LTRR601 pKa = 11.84 TATVVVNEE609 pKa = 4.27 PSPGSGYY616 pKa = 10.54 LQAFVDD622 pKa = 4.41 FGGDD626 pKa = 3.45 GLFLGDD632 pKa = 4.83 QVATDD637 pKa = 3.73 IQDD640 pKa = 3.95 GGPQDD645 pKa = 4.7 LDD647 pKa = 3.5 GAVNGEE653 pKa = 3.67 ISFPISVPATAVLTPTFVRR672 pKa = 11.84 LRR674 pKa = 11.84 WSSAAGLDD682 pKa = 3.83 ALTAAPDD689 pKa = 4.19 GEE691 pKa = 4.38 VEE693 pKa = 5.09 DD694 pKa = 4.18 YY695 pKa = 11.27 GITIKK700 pKa = 10.04 TPPDD704 pKa = 3.72 ADD706 pKa = 4.12 RR707 pKa = 11.84 GDD709 pKa = 4.02 APASYY714 pKa = 10.82 GDD716 pKa = 3.67 PQHH719 pKa = 7.62 IIEE722 pKa = 5.07 GPGAPEE728 pKa = 4.28 VYY730 pKa = 10.25 LGTVPPDD737 pKa = 3.23 IDD739 pKa = 5.23 LLAQNSIGATGDD751 pKa = 3.73 DD752 pKa = 4.11 ADD754 pKa = 5.2 GSDD757 pKa = 4.75 DD758 pKa = 3.66 EE759 pKa = 6.33 DD760 pKa = 4.66 GVVLPQFYY768 pKa = 10.39 RR769 pKa = 11.84 GGLAEE774 pKa = 4.28 IAVTVNDD781 pKa = 3.55 RR782 pKa = 11.84 SGAPRR787 pKa = 11.84 SSYY790 pKa = 10.63 LQAFIDD796 pKa = 4.1 FNGDD800 pKa = 3.16 GTFGQAGEE808 pKa = 4.2 QVALNLQDD816 pKa = 4.29 GDD818 pKa = 5.38 AIDD821 pKa = 5.3 KK822 pKa = 11.09 DD823 pKa = 3.91 GATNGSILFEE833 pKa = 4.24 VAVPAGATTLPTYY846 pKa = 11.12 ARR848 pKa = 11.84 FRR850 pKa = 11.84 WSTDD854 pKa = 2.97 SAGLATAFEE863 pKa = 5.05 GEE865 pKa = 4.33 VEE867 pKa = 5.13 DD868 pKa = 3.89 YY869 pKa = 10.61 TLTISSDD876 pKa = 3.91 PPPLVCDD883 pKa = 3.85 ASLYY887 pKa = 10.84 AIVDD891 pKa = 3.92 SPSTLIRR898 pKa = 11.84 MTFRR902 pKa = 11.84 DD903 pKa = 3.47 AGGSYY908 pKa = 9.14 ATTVDD913 pKa = 3.66 PSGSTGEE920 pKa = 4.24 NRR922 pKa = 11.84 DD923 pKa = 3.6 GAWGYY928 pKa = 10.32 NALDD932 pKa = 4.1 GYY934 pKa = 10.72 IYY936 pKa = 10.57 GVEE939 pKa = 3.99 EE940 pKa = 3.77 NTRR943 pKa = 11.84 RR944 pKa = 11.84 LYY946 pKa = 10.84 RR947 pKa = 11.84 IDD949 pKa = 3.45 GAGNIADD956 pKa = 4.71 LGNISGADD964 pKa = 3.52 DD965 pKa = 4.22 ALNAGDD971 pKa = 4.32 ILPNGRR977 pKa = 11.84 FIYY980 pKa = 10.37 EE981 pKa = 3.52 VDD983 pKa = 3.21 ANTWQIVDD991 pKa = 4.35 LTNPTAPIGLGAIALTIGVNPEE1013 pKa = 3.77 DD1014 pKa = 4.06 LAFNPVDD1021 pKa = 3.99 GIMYY1025 pKa = 10.39 GIDD1028 pKa = 3.37 RR1029 pKa = 11.84 NSGFLFRR1036 pKa = 11.84 AAVNNGTPGSVTPQAIGPAVYY1057 pKa = 10.59 AGMFDD1062 pKa = 4.52 ALWFDD1067 pKa = 3.49 RR1068 pKa = 11.84 DD1069 pKa = 3.31 GRR1071 pKa = 11.84 LYY1073 pKa = 10.86 GYY1075 pKa = 11.32 SNTTNNLFIISTATGVPRR1093 pKa = 11.84 LISNIPGDD1101 pKa = 3.69 EE1102 pKa = 4.08 GDD1104 pKa = 3.71 RR1105 pKa = 11.84 SDD1107 pKa = 5.64 GISCRR1112 pKa = 11.84 GPAPIPLGGISGNIYY1127 pKa = 10.52 EE1128 pKa = 5.25 DD1129 pKa = 4.07 ADD1131 pKa = 3.75 ASDD1134 pKa = 4.1 VKK1136 pKa = 11.24 DD1137 pKa = 3.46 SGEE1140 pKa = 4.34 TNFGAGVGISVYY1152 pKa = 11.05 ADD1154 pKa = 2.85 NGTPANTADD1163 pKa = 4.32 DD1164 pKa = 4.28 VFLVTTDD1171 pKa = 3.31 TLADD1175 pKa = 3.31 GTYY1178 pKa = 10.96 AVGDD1182 pKa = 3.63 LMVNTTYY1189 pKa = 10.74 RR1190 pKa = 11.84 VALDD1194 pKa = 3.86 EE1195 pKa = 5.16 ADD1197 pKa = 3.97 ADD1199 pKa = 4.25 LPSGLTIGTSNPLIGVAVSGNSVTTDD1225 pKa = 2.75 QDD1227 pKa = 3.61 FGFDD1231 pKa = 3.63 PAGSDD1236 pKa = 4.64 LEE1238 pKa = 4.32 LTKK1241 pKa = 10.35 IAAATGTITPITNVSEE1257 pKa = 4.16 GDD1259 pKa = 3.63 TIDD1262 pKa = 3.63 WIITITNVSGGSPSGVKK1279 pKa = 10.51 VIDD1282 pKa = 3.91 QIPSGFAYY1290 pKa = 10.69 VSDD1293 pKa = 4.76 DD1294 pKa = 3.83 APSTGDD1300 pKa = 3.23 TYY1302 pKa = 11.75 DD1303 pKa = 4.03 PNTGLWFVDD1312 pKa = 3.74 EE1313 pKa = 4.7 LLSGASEE1320 pKa = 4.24 TLTITTTVLGSGDD1333 pKa = 3.63 FTNQAEE1339 pKa = 4.73 IIYY1342 pKa = 10.38 SSLPDD1347 pKa = 4.47 PDD1349 pKa = 4.77 SDD1351 pKa = 4.64 PNTGPLTDD1359 pKa = 5.22 DD1360 pKa = 5.62 LFDD1363 pKa = 4.06 QLPDD1367 pKa = 4.15 DD1368 pKa = 5.46 DD1369 pKa = 4.34 EE1370 pKa = 6.53 ASYY1373 pKa = 11.37 SVNLVTGEE1381 pKa = 4.38 RR1382 pKa = 11.84 ILSGRR1387 pKa = 11.84 VFIDD1391 pKa = 3.0 NGAGGGTAHH1400 pKa = 7.69 DD1401 pKa = 4.06 ALRR1404 pKa = 11.84 NGSEE1408 pKa = 4.2 SGASSAVFEE1417 pKa = 4.76 ILDD1420 pKa = 3.47 SSGTVLATPGVAADD1434 pKa = 4.15 GTWSYY1439 pKa = 11.21 GLSGTYY1445 pKa = 10.01 SGAITIRR1452 pKa = 11.84 AIPANDD1458 pKa = 3.03 YY1459 pKa = 10.66 RR1460 pKa = 11.84 AISEE1464 pKa = 4.33 ATSGLPDD1471 pKa = 3.82 LVDD1474 pKa = 4.61 ANPHH1478 pKa = 6.18 DD1479 pKa = 4.45 GEE1481 pKa = 4.53 FTFTPEE1487 pKa = 3.77 AFGNRR1492 pKa = 11.84 MGLDD1496 pKa = 2.87 IGLLQLPSLTNDD1508 pKa = 2.77 QTTTVAQGQVATLPHH1523 pKa = 7.47 IYY1525 pKa = 9.14 TATSEE1530 pKa = 4.38 GQVTFSFAGATSTPTGAFNAALYY1553 pKa = 10.44 QDD1555 pKa = 4.24 VGCDD1559 pKa = 3.61 DD1560 pKa = 4.81 SLDD1563 pKa = 3.89 GPITGPIAVITGQTICIVSRR1583 pKa = 11.84 VSAGSGAGQGSTYY1596 pKa = 10.5 VYY1598 pKa = 10.45 QVLAATAFARR1608 pKa = 11.84 TTVTSSVSNTDD1619 pKa = 3.6 EE1620 pKa = 4.28 ISVGGQSTQIEE1631 pKa = 4.17 LRR1633 pKa = 11.84 KK1634 pKa = 7.86 TVEE1637 pKa = 4.02 NEE1639 pKa = 3.98 TQGTLEE1645 pKa = 3.98 GTTNLGSINDD1655 pKa = 3.47 ILLYY1659 pKa = 10.35 RR1660 pKa = 11.84 IYY1662 pKa = 10.72 LRR1664 pKa = 11.84 NKK1666 pKa = 9.05 ATTQASNVKK1675 pKa = 9.32 IYY1677 pKa = 11.35 DD1678 pKa = 3.28 MTPPYY1683 pKa = 10.04 TSLSEE1688 pKa = 4.64 PIVDD1692 pKa = 3.85 PQQVSPNLNCDD1703 pKa = 3.64 LVRR1706 pKa = 11.84 PASNVASYY1714 pKa = 11.18 AGAIEE1719 pKa = 4.35 WNCTGQMLPGEE1730 pKa = 4.3 TGAVQFRR1737 pKa = 11.84 VGIQQ1741 pKa = 2.88
Molecular weight: 181.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.675
IPC2_protein 3.681
IPC_protein 3.745
Toseland 3.503
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.681
Rodwell 3.567
Grimsley 3.414
Solomon 3.745
Lehninger 3.694
Nozaki 3.846
DTASelect 4.126
Thurlkill 3.554
EMBOSS 3.694
Sillero 3.872
Patrickios 1.38
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.773
Protein with the highest isoelectric point:
>tr|A9E5V9|A9E5V9_9RHOB Cysteine--tRNA ligase OS=Sulfitobacter indolifex HEL-45 OX=391624 GN=cysS PE=3 SV=1
MM1 pKa = 7.23 NSIVNMVMRR10 pKa = 11.84 QVMRR14 pKa = 11.84 RR15 pKa = 11.84 LVSRR19 pKa = 11.84 GIGAGTRR26 pKa = 11.84 RR27 pKa = 11.84 AGKK30 pKa = 9.92 RR31 pKa = 11.84 GSGKK35 pKa = 10.46 GGAFNARR42 pKa = 11.84 TAQQGIRR49 pKa = 11.84 LLRR52 pKa = 11.84 RR53 pKa = 11.84 FFRR56 pKa = 11.84 SKK58 pKa = 10.99
Molecular weight: 6.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.647
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.369
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.218
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4147
0
4147
1216087
20
2148
293.2
31.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.224 ± 0.055
0.89 ± 0.011
6.095 ± 0.034
5.987 ± 0.039
3.693 ± 0.024
8.533 ± 0.039
2.07 ± 0.021
5.223 ± 0.027
3.297 ± 0.031
10.07 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.898 ± 0.019
2.709 ± 0.019
4.971 ± 0.024
3.369 ± 0.021
6.466 ± 0.037
5.199 ± 0.025
5.548 ± 0.027
7.185 ± 0.032
1.349 ± 0.017
2.221 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here