candidate division MSBL1 archaeon SCGC-AAA259J03

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 964 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A656YXA3|A0A656YXA3_9EURY eIF-5a domain-containing protein OS=candidate division MSBL1 archaeon SCGC-AAA259J03 OX=1698269 GN=AKJ39_00640 PE=3 SV=1
MM1 pKa = 8.32DD2 pKa = 3.77SRR4 pKa = 11.84KK5 pKa = 9.53IVVVAVLGVLGLGLFSTFSARR26 pKa = 11.84SSATATLSAGADD38 pKa = 3.1ATGFVDD44 pKa = 5.08NIIYY48 pKa = 9.3PYY50 pKa = 10.98SDD52 pKa = 4.09DD53 pKa = 4.84LNNDD57 pKa = 3.05RR58 pKa = 11.84AGNLKK63 pKa = 10.34YY64 pKa = 10.44GQEE67 pKa = 4.34VTWDD71 pKa = 4.53AIDD74 pKa = 3.95DD75 pKa = 4.02TATGIDD81 pKa = 3.77NGDD84 pKa = 3.72LYY86 pKa = 11.16LINLTDD92 pKa = 3.25SDD94 pKa = 4.71YY95 pKa = 11.69AGEE98 pKa = 4.25LLVTLYY104 pKa = 7.46MTNPDD109 pKa = 3.93DD110 pKa = 3.95VAAKK114 pKa = 9.61YY115 pKa = 10.52SYY117 pKa = 10.81WNAQIDD123 pKa = 4.43AYY125 pKa = 9.41TLEE128 pKa = 4.35NTWDD132 pKa = 3.53NLDD135 pKa = 3.63NKK137 pKa = 10.57DD138 pKa = 4.91RR139 pKa = 11.84LGDD142 pKa = 3.51WSSDD146 pKa = 3.42DD147 pKa = 3.85EE148 pKa = 5.51NIMLSLSQGYY158 pKa = 7.12VTWRR162 pKa = 11.84VGRR165 pKa = 11.84DD166 pKa = 3.59SEE168 pKa = 4.31DD169 pKa = 3.37HH170 pKa = 6.64LVDD173 pKa = 4.06IVIEE177 pKa = 4.74DD178 pKa = 4.39GSCYY182 pKa = 8.3TTDD185 pKa = 3.17STKK188 pKa = 10.4TDD190 pKa = 3.09SKK192 pKa = 10.95YY193 pKa = 11.15DD194 pKa = 3.34PSFYY198 pKa = 9.87ITVQQAA204 pKa = 2.56

Molecular weight: 22.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A656YVY8|A0A656YVY8_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA259J03 OX=1698269 GN=AKJ39_02720 PE=4 SV=1
PP1 pKa = 7.3SRR3 pKa = 11.84HH4 pKa = 6.35CDD6 pKa = 3.11SVPPHH11 pKa = 7.07PPRR14 pKa = 11.84AGKK17 pKa = 7.85TGKK20 pKa = 9.63RR21 pKa = 11.84DD22 pKa = 3.35RR23 pKa = 11.84EE24 pKa = 3.81EE25 pKa = 4.67RR26 pKa = 11.84DD27 pKa = 2.93LGKK30 pKa = 10.48YY31 pKa = 9.49RR32 pKa = 11.84EE33 pKa = 4.43AVRR36 pKa = 11.84SVRR39 pKa = 11.84DD40 pKa = 3.62RR41 pKa = 11.84APSHH45 pKa = 6.15CPPRR49 pKa = 11.84EE50 pKa = 3.63IGASLFPFPLEE61 pKa = 3.59AKK63 pKa = 9.59RR64 pKa = 11.84WIRR67 pKa = 11.84EE68 pKa = 3.62EE69 pKa = 3.74GRR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84MM74 pKa = 3.92

Molecular weight: 8.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

964

0

964

208237

33

1105

216.0

24.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.148 ± 0.076

0.999 ± 0.039

5.81 ± 0.081

10.421 ± 0.11

3.911 ± 0.068

7.516 ± 0.086

1.708 ± 0.036

6.972 ± 0.073

7.493 ± 0.084

9.003 ± 0.092

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.272 ± 0.035

3.711 ± 0.061

4.169 ± 0.055

2.164 ± 0.04

5.912 ± 0.085

6.193 ± 0.068

4.752 ± 0.06

6.913 ± 0.071

1.079 ± 0.031

2.852 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski