Dragonfly larvae associated circular virus-6
Average proteome isoelectric point is 8.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W5U2Q2|W5U2Q2_9VIRU Coat protein OS=Dragonfly larvae associated circular virus-6 OX=1454027 PE=4 SV=1
MM1 pKa = 7.72 ACRR4 pKa = 11.84 SRR6 pKa = 11.84 GWAFTLFHH14 pKa = 5.88 YY15 pKa = 9.89 TEE17 pKa = 4.1 EE18 pKa = 5.39 DD19 pKa = 2.96 INKK22 pKa = 9.4 IKK24 pKa = 10.6 KK25 pKa = 10.57 LEE27 pKa = 3.94 VQYY30 pKa = 11.07 QCVGKK35 pKa = 9.56 EE36 pKa = 3.58 ICPKK40 pKa = 10.41 SKK42 pKa = 10.19 EE43 pKa = 4.1 PHH45 pKa = 5.24 LQGYY49 pKa = 9.16 LYY51 pKa = 10.18 YY52 pKa = 10.29 ANPVRR57 pKa = 11.84 FEE59 pKa = 4.15 TVKK62 pKa = 9.66 TSIRR66 pKa = 11.84 KK67 pKa = 8.32 EE68 pKa = 3.78 VHH70 pKa = 6.25 LEE72 pKa = 3.72 SARR75 pKa = 11.84 YY76 pKa = 7.66 SAKK79 pKa = 10.48 RR80 pKa = 11.84 NARR83 pKa = 11.84 YY84 pKa = 9.35 CKK86 pKa = 10.27 KK87 pKa = 10.67 EE88 pKa = 3.89 GDD90 pKa = 2.95 WWEE93 pKa = 4.05 QGEE96 pKa = 4.44 PPMMGARR103 pKa = 11.84 SDD105 pKa = 3.79 LAEE108 pKa = 4.03 VALMVRR114 pKa = 11.84 NGASLEE120 pKa = 4.13 QVAEE124 pKa = 4.31 EE125 pKa = 4.01 NPEE128 pKa = 3.91 VYY130 pKa = 10.11 VRR132 pKa = 11.84 YY133 pKa = 10.19 SRR135 pKa = 11.84 GLQALQTKK143 pKa = 7.82 VMKK146 pKa = 10.55 HH147 pKa = 3.4 RR148 pKa = 11.84 TEE150 pKa = 4.15 RR151 pKa = 11.84 PNVTWIWGSTGVGKK165 pKa = 8.41 TRR167 pKa = 11.84 YY168 pKa = 9.5 AVEE171 pKa = 4.44 LCDD174 pKa = 3.83 SFYY177 pKa = 11.01 MKK179 pKa = 10.62 DD180 pKa = 3.21 GTKK183 pKa = 8.85 WWDD186 pKa = 3.55 GYY188 pKa = 8.38 EE189 pKa = 3.91 QQKK192 pKa = 10.18 WIILDD197 pKa = 3.77 DD198 pKa = 3.77 FHH200 pKa = 7.64 WDD202 pKa = 3.17 GKK204 pKa = 10.41 VEE206 pKa = 4.15 SFRR209 pKa = 11.84 EE210 pKa = 4.09 FLRR213 pKa = 11.84 MIDD216 pKa = 3.27 RR217 pKa = 11.84 YY218 pKa = 10.43 KK219 pKa = 10.92 YY220 pKa = 9.64 QGEE223 pKa = 4.49 TKK225 pKa = 10.29 GGHH228 pKa = 5.02 VKK230 pKa = 10.25 INSPQIVITCEE241 pKa = 3.47 HH242 pKa = 6.82 APDD245 pKa = 4.02 KK246 pKa = 10.7 FWYY249 pKa = 7.61 DD250 pKa = 3.32 TKK252 pKa = 10.94 LDD254 pKa = 3.64 QVIRR258 pKa = 11.84 RR259 pKa = 11.84 LNKK262 pKa = 9.46 VIHH265 pKa = 6.14 LSGTEE270 pKa = 4.0 VVGTEE275 pKa = 4.22 VPGNTIPEE283 pKa = 4.3 PQNSVAAGAAPPPPQII299 pKa = 4.46
Molecular weight: 34.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.343
IPC2_protein 7.702
IPC_protein 7.614
Toseland 7.366
ProMoST 8.126
Dawson 8.317
Bjellqvist 8.58
Wikipedia 8.258
Rodwell 8.346
Grimsley 7.395
Solomon 8.434
Lehninger 8.434
Nozaki 8.712
DTASelect 8.361
Thurlkill 8.419
EMBOSS 8.521
Sillero 8.712
Patrickios 4.329
IPC_peptide 8.419
IPC2_peptide 7.483
IPC2.peptide.svr19 7.699
Protein with the highest isoelectric point:
>tr|W5U2Q2|W5U2Q2_9VIRU Coat protein OS=Dragonfly larvae associated circular virus-6 OX=1454027 PE=4 SV=1
MM1 pKa = 8.0 PFRR4 pKa = 11.84 KK5 pKa = 9.41 RR6 pKa = 11.84 AVRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 PFKK15 pKa = 9.57 RR16 pKa = 11.84 TRR18 pKa = 11.84 TYY20 pKa = 9.7 RR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 IMRR27 pKa = 11.84 PGRR30 pKa = 11.84 SLMVHH35 pKa = 4.68 SFKK38 pKa = 11.02 RR39 pKa = 11.84 KK40 pKa = 8.34 VNYY43 pKa = 10.36 GSFDD47 pKa = 3.71 FDD49 pKa = 4.38 SGTNGYY55 pKa = 10.33 KK56 pKa = 10.19 SGALTFRR63 pKa = 11.84 LSDD66 pKa = 3.34 VPGYY70 pKa = 11.4 AEE72 pKa = 5.12 FGPMYY77 pKa = 10.44 DD78 pKa = 3.88 KK79 pKa = 11.11 FRR81 pKa = 11.84 IDD83 pKa = 3.35 YY84 pKa = 10.04 VKK86 pKa = 10.76 LYY88 pKa = 9.05 FCCRR92 pKa = 11.84 YY93 pKa = 8.23 NTNSYY98 pKa = 11.43 AEE100 pKa = 4.35 TASATYY106 pKa = 9.88 QVGSIHH112 pKa = 6.39 GLVVVDD118 pKa = 4.83 TDD120 pKa = 4.33 DD121 pKa = 3.91 EE122 pKa = 5.0 TPVTQDD128 pKa = 2.91 EE129 pKa = 4.66 SGMNDD134 pKa = 2.98 MRR136 pKa = 11.84 QHH138 pKa = 6.17 PRR140 pKa = 11.84 AKK142 pKa = 10.23 SHH144 pKa = 6.37 IFGNGKK150 pKa = 8.32 SQILSMGCKK159 pKa = 9.47 PRR161 pKa = 11.84 ILSEE165 pKa = 4.12 VYY167 pKa = 9.97 RR168 pKa = 11.84 SGIATSYY175 pKa = 10.89 NPIRR179 pKa = 11.84 APFLSYY185 pKa = 10.91 GSNGFSIPHH194 pKa = 5.98 YY195 pKa = 9.67 GVKK198 pKa = 10.15 FCFRR202 pKa = 11.84 LPTTGSALNPFKK214 pKa = 11.39 VPMDD218 pKa = 3.73 VFATFYY224 pKa = 11.17 LSMRR228 pKa = 11.84 DD229 pKa = 3.61 PKK231 pKa = 11.21
Molecular weight: 26.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.299
IPC2_protein 9.721
IPC_protein 10.277
Toseland 10.131
ProMoST 9.984
Dawson 10.379
Bjellqvist 10.116
Wikipedia 10.599
Rodwell 10.613
Grimsley 10.482
Solomon 10.409
Lehninger 10.365
Nozaki 10.145
DTASelect 10.116
Thurlkill 10.218
EMBOSS 10.555
Sillero 10.292
Patrickios 9.926
IPC_peptide 10.409
IPC2_peptide 9.107
IPC2.peptide.svr19 8.584
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
530
231
299
265.0
30.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.472 ± 0.478
1.887 ± 0.104
4.717 ± 0.322
5.849 ± 2.479
4.717 ± 1.778
7.358 ± 0.292
2.453 ± 0.194
4.528 ± 0.425
6.981 ± 0.911
5.094 ± 0.224
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.019 ± 0.59
3.585 ± 0.209
5.849 ± 0.434
3.396 ± 1.12
8.302 ± 1.696
6.604 ± 1.674
5.66 ± 0.269
6.981 ± 0.619
1.887 ± 1.27
5.66 ± 0.561
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here