Listeria fleischmannii subsp. fleischmannii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria; Listeria fleischmannii

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2943 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2X3GIY1|A0A2X3GIY1_9LIST Metalloprotease ypwA OS=Listeria fleischmannii subsp. fleischmannii OX=1671902 GN=ypwA_1 PE=4 SV=1
MM1 pKa = 7.44AKK3 pKa = 10.4VMIVYY8 pKa = 10.7ASMTGNTQEE17 pKa = 4.07IADD20 pKa = 3.77ILGEE24 pKa = 4.09EE25 pKa = 4.34LEE27 pKa = 4.55KK28 pKa = 11.18YY29 pKa = 10.33DD30 pKa = 3.73IEE32 pKa = 4.81VEE34 pKa = 3.8IEE36 pKa = 3.71EE37 pKa = 5.03CINVEE42 pKa = 4.32PEE44 pKa = 4.35DD45 pKa = 3.94LLDD48 pKa = 3.95YY49 pKa = 11.2DD50 pKa = 3.96GALIGGYY57 pKa = 8.69TYY59 pKa = 11.29DD60 pKa = 5.36DD61 pKa = 3.82GQLPDD66 pKa = 4.05EE67 pKa = 4.84FVDD70 pKa = 5.13FYY72 pKa = 11.52EE73 pKa = 5.95DD74 pKa = 3.17MAEE77 pKa = 3.84VDD79 pKa = 4.7FSGKK83 pKa = 9.41VCASFGSGDD92 pKa = 3.27TFYY95 pKa = 11.39DD96 pKa = 4.14EE97 pKa = 4.93FCLTVDD103 pKa = 4.72LVEE106 pKa = 4.45TRR108 pKa = 11.84LKK110 pKa = 10.31EE111 pKa = 3.85QGATVPVAGLKK122 pKa = 10.14VDD124 pKa = 5.13LDD126 pKa = 3.89PDD128 pKa = 3.61EE129 pKa = 6.72DD130 pKa = 3.86DD131 pKa = 3.84VEE133 pKa = 4.24RR134 pKa = 11.84AEE136 pKa = 5.49AYY138 pKa = 10.05AKK140 pKa = 10.12TYY142 pKa = 11.22VDD144 pKa = 3.93TLLKK148 pKa = 10.76

Molecular weight: 16.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2X3GKT2|A0A2X3GKT2_9LIST Transketolase OS=Listeria fleischmannii subsp. fleischmannii OX=1671902 GN=tkt_4 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.29QPSKK9 pKa = 9.59RR10 pKa = 11.84KK11 pKa = 9.09RR12 pKa = 11.84KK13 pKa = 8.86KK14 pKa = 8.28VHH16 pKa = 5.49GFRR19 pKa = 11.84TRR21 pKa = 11.84MSSKK25 pKa = 9.7NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2943

0

2943

801550

29

1658

272.4

30.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.443 ± 0.047

0.615 ± 0.014

5.203 ± 0.037

7.254 ± 0.06

4.876 ± 0.042

6.646 ± 0.045

1.894 ± 0.024

7.788 ± 0.049

7.218 ± 0.044

9.834 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.772 ± 0.022

4.507 ± 0.038

3.369 ± 0.025

3.489 ± 0.027

3.955 ± 0.034

5.923 ± 0.037

5.831 ± 0.045

6.969 ± 0.038

0.87 ± 0.017

3.541 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski