bacterium AMD02

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 810 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q0AHR7|A0A4Q0AHR7_9BACT ParB/RepB/Spo0J family partition protein OS=bacterium AMD02 OX=2506948 GN=EOT05_02310 PE=3 SV=1
MM1 pKa = 7.45RR2 pKa = 11.84VVVMYY7 pKa = 10.65KK8 pKa = 10.12EE9 pKa = 3.89QTDD12 pKa = 3.65YY13 pKa = 11.35ARR15 pKa = 11.84QVIEE19 pKa = 4.36YY20 pKa = 9.39IADD23 pKa = 4.53FKK25 pKa = 11.24RR26 pKa = 11.84QTGHH30 pKa = 7.37DD31 pKa = 4.41LEE33 pKa = 4.72TMNPEE38 pKa = 3.97SPAGIDD44 pKa = 3.27FTQVYY49 pKa = 10.53DD50 pKa = 3.68IMEE53 pKa = 5.11LPTMIALSDD62 pKa = 4.02DD63 pKa = 3.35GVMQNTWSGLPLPTISEE80 pKa = 3.94VSYY83 pKa = 10.37YY84 pKa = 9.98IQQ86 pKa = 4.22

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1J7G1|A0A4V1J7G1_9BACT Uncharacterized protein OS=bacterium AMD02 OX=2506948 GN=EOT05_02345 PE=4 SV=1
MM1 pKa = 7.86PKK3 pKa = 9.64MKK5 pKa = 8.79THH7 pKa = 6.91KK8 pKa = 9.69GTAKK12 pKa = 9.99RR13 pKa = 11.84VKK15 pKa = 8.9ITSTGKK21 pKa = 8.05LTRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84AFGAHH31 pKa = 6.75LLAKK35 pKa = 10.0KK36 pKa = 10.66SNGRR40 pKa = 11.84KK41 pKa = 9.13RR42 pKa = 11.84RR43 pKa = 11.84IGTTAVVTGHH53 pKa = 5.5TARR56 pKa = 11.84NIKK59 pKa = 9.93RR60 pKa = 11.84ALGII64 pKa = 3.94

Molecular weight: 7.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

810

0

810

236896

38

1281

292.5

32.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.971 ± 0.09

0.423 ± 0.023

5.481 ± 0.076

5.684 ± 0.096

3.774 ± 0.055

6.919 ± 0.073

2.022 ± 0.043

7.154 ± 0.08

5.918 ± 0.08

9.278 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.168 ± 0.037

4.219 ± 0.057

4.033 ± 0.066

3.786 ± 0.044

5.136 ± 0.081

6.451 ± 0.081

6.791 ± 0.096

7.467 ± 0.061

1.07 ± 0.037

3.255 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski