Streptococcus phage Javan539

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AHM3|A0A4D6AHM3_9CAUD Uncharacterized protein OS=Streptococcus phage Javan539 OX=2548250 GN=Javan539_0007 PE=4 SV=1
MM1 pKa = 7.45NNNDD5 pKa = 3.19FSEE8 pKa = 4.25VLKK11 pKa = 11.15DD12 pKa = 4.0FLAGLGLPLTPRR24 pKa = 11.84LDD26 pKa = 3.76YY27 pKa = 11.43LNEE30 pKa = 4.34GEE32 pKa = 5.06DD33 pKa = 3.76LVIYY37 pKa = 9.34ALPGGKK43 pKa = 9.3VEE45 pKa = 4.77DD46 pKa = 4.02EE47 pKa = 4.68DD48 pKa = 3.96MAGTQILSLPYY59 pKa = 10.19EE60 pKa = 4.01IAIKK64 pKa = 10.82SKK66 pKa = 10.64DD67 pKa = 3.62QQKK70 pKa = 10.59VNATLWKK77 pKa = 10.39INTEE81 pKa = 3.79LSKK84 pKa = 10.74IGLEE88 pKa = 4.12LPSLNNSYY96 pKa = 8.6TFLSLKK102 pKa = 10.68VEE104 pKa = 4.42TPSLNDD110 pKa = 3.41VNDD113 pKa = 3.29QDD115 pKa = 4.21YY116 pKa = 11.22YY117 pKa = 10.75IYY119 pKa = 11.07LLDD122 pKa = 3.94LQATIEE128 pKa = 4.22VEE130 pKa = 3.84RR131 pKa = 11.84SLNN134 pKa = 3.44

Molecular weight: 15.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AHP5|A0A4D6AHP5_9CAUD Transcriptional terminator OS=Streptococcus phage Javan539 OX=2548250 GN=Javan539_0027 PE=4 SV=1
MM1 pKa = 7.51RR2 pKa = 11.84AKK4 pKa = 10.4EE5 pKa = 3.6FALYY9 pKa = 10.25KK10 pKa = 10.72GEE12 pKa = 4.33EE13 pKa = 4.22LLAMGTKK20 pKa = 9.99RR21 pKa = 11.84EE22 pKa = 3.93IAKK25 pKa = 10.07QLGVSASTVGYY36 pKa = 10.25YY37 pKa = 7.55GTPVYY42 pKa = 10.73ARR44 pKa = 11.84RR45 pKa = 11.84TSEE48 pKa = 3.42NGRR51 pKa = 11.84RR52 pKa = 11.84LVEE55 pKa = 3.93LL56 pKa = 4.13

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

11341

41

1535

276.6

30.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.456 ± 1.1

0.423 ± 0.08

6.234 ± 0.574

6.869 ± 0.739

3.774 ± 0.223

6.842 ± 0.5

1.287 ± 0.181

6.569 ± 0.327

7.336 ± 0.517

7.83 ± 0.38

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.786 ± 0.442

5.194 ± 0.395

3.545 ± 0.486

4.612 ± 0.339

3.862 ± 0.411

6.163 ± 0.659

6.816 ± 0.355

6.631 ± 0.338

1.226 ± 0.16

3.545 ± 0.457
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski