Paenibacillus methanolicus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6989 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5S5C6Y4|A0A5S5C6Y4_9BACL Diguanylate cyclase (GGDEF)-like protein OS=Paenibacillus methanolicus OX=582686 GN=BCM02_106438 PE=4 SV=1
MM1 pKa = 7.45QGLFVLNTRR10 pKa = 11.84TIGRR14 pKa = 11.84KK15 pKa = 9.08IGAALLAASLALPGSVIYY33 pKa = 10.77AEE35 pKa = 5.39EE36 pKa = 5.37GDD38 pKa = 4.5DD39 pKa = 4.84WIPDD43 pKa = 3.55PPAAISFDD51 pKa = 3.83APVVTVDD58 pKa = 3.34EE59 pKa = 4.75GAGYY63 pKa = 10.62AYY65 pKa = 10.63VGVSRR70 pKa = 11.84TGAASSLTVDD80 pKa = 3.31YY81 pKa = 8.8ATYY84 pKa = 10.76GGTATEE90 pKa = 4.31GTDD93 pKa = 3.32YY94 pKa = 11.11LAASGQITFGPGEE107 pKa = 4.08TFATIEE113 pKa = 4.01IPITNDD119 pKa = 2.56IQFEE123 pKa = 4.12PAEE126 pKa = 4.32SFSVEE131 pKa = 4.22LFNVSDD137 pKa = 4.13GVTSIDD143 pKa = 3.52RR144 pKa = 11.84GTTTVTIADD153 pKa = 4.09NDD155 pKa = 3.84PDD157 pKa = 3.81VPYY160 pKa = 11.21NPGKK164 pKa = 10.7LGFATALTEE173 pKa = 3.95VDD175 pKa = 3.98EE176 pKa = 4.2NHH178 pKa = 7.39PDD180 pKa = 3.37GTVSLTVARR189 pKa = 11.84SEE191 pKa = 4.2GTDD194 pKa = 3.44GYY196 pKa = 11.44VAVDD200 pKa = 3.41YY201 pKa = 11.27QIDD204 pKa = 3.76GGTAGNGADD213 pKa = 4.0FEE215 pKa = 4.97GTGGTLSFEE224 pKa = 4.7PGEE227 pKa = 4.19SSKK230 pKa = 9.96TIQVTLYY237 pKa = 10.9DD238 pKa = 4.04DD239 pKa = 4.77AEE241 pKa = 4.66SEE243 pKa = 4.36GNEE246 pKa = 4.04YY247 pKa = 10.74FHH249 pKa = 7.33LSLSNPTNDD258 pKa = 2.83ATLAEE263 pKa = 4.34SSFTKK268 pKa = 10.45VRR270 pKa = 11.84ILDD273 pKa = 3.77NEE275 pKa = 3.98PWTPRR280 pKa = 11.84AGSFEE285 pKa = 4.53VNPTAPMGEE294 pKa = 3.99ASGTHH299 pKa = 6.19YY300 pKa = 10.96INVIRR305 pKa = 11.84TDD307 pKa = 3.56GADD310 pKa = 3.04VEE312 pKa = 4.54AAIDD316 pKa = 3.67YY317 pKa = 7.62TIAGVTAEE325 pKa = 4.63EE326 pKa = 4.99GSDD329 pKa = 3.58FTGSSGTLVFPVDD342 pKa = 3.48EE343 pKa = 4.23TLQYY347 pKa = 10.66IPLPILDD354 pKa = 4.7DD355 pKa = 4.25ALSEE359 pKa = 4.29GNEE362 pKa = 4.21TLTITLSNPTSGAVIGTYY380 pKa = 7.19GTRR383 pKa = 11.84TYY385 pKa = 10.19TIEE388 pKa = 4.42DD389 pKa = 3.85NEE391 pKa = 4.19PHH393 pKa = 6.63EE394 pKa = 4.39EE395 pKa = 3.89QHH397 pKa = 6.77PGMFMLGASQYY408 pKa = 11.5AVDD411 pKa = 4.36EE412 pKa = 4.66NGGSAKK418 pKa = 10.58LQVTRR423 pKa = 11.84MDD425 pKa = 3.92GADD428 pKa = 3.54GEE430 pKa = 4.59ATLTYY435 pKa = 10.42SILGGSAAEE444 pKa = 4.12NADD447 pKa = 3.44YY448 pKa = 11.08AGNSGQLTFADD459 pKa = 4.32GEE461 pKa = 4.51TEE463 pKa = 4.16KK464 pKa = 10.9FIEE467 pKa = 4.29VPIVDD472 pKa = 4.58DD473 pKa = 3.93SMHH476 pKa = 5.89EE477 pKa = 4.05AEE479 pKa = 4.7EE480 pKa = 4.07QFQVRR485 pKa = 11.84LLDD488 pKa = 3.61ATGGAVVGPNYY499 pKa = 10.48LSGVTIKK506 pKa = 11.1DD507 pKa = 3.28NDD509 pKa = 4.13PAGVLQFSPFTTTVYY524 pKa = 10.66EE525 pKa = 4.06EE526 pKa = 4.4SKK528 pKa = 10.5QVTMTVARR536 pKa = 11.84TGDD539 pKa = 3.42TSGTASVAYY548 pKa = 8.24ATKK551 pKa = 10.22DD552 pKa = 3.44YY553 pKa = 11.11SALAGRR559 pKa = 11.84DD560 pKa = 3.34YY561 pKa = 11.2AAQSGVLTFAPGEE574 pKa = 4.25TQKK577 pKa = 11.23KK578 pKa = 7.35ITITMYY584 pKa = 10.93DD585 pKa = 3.66DD586 pKa = 4.47SVKK589 pKa = 10.48EE590 pKa = 3.75WFEE593 pKa = 3.92YY594 pKa = 10.6FSILLSNPAGAEE606 pKa = 3.77LGTYY610 pKa = 8.19STGLVYY616 pKa = 10.81VYY618 pKa = 11.11DD619 pKa = 4.01NDD621 pKa = 3.86KK622 pKa = 9.63YY623 pKa = 9.46TYY625 pKa = 7.31PTWPRR630 pKa = 11.84YY631 pKa = 9.0

Molecular weight: 66.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5S5C7S6|A0A5S5C7S6_9BACL Putative NADPH-quinone reductase OS=Paenibacillus methanolicus OX=582686 GN=BCM02_106324 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.48GFRR19 pKa = 11.84KK20 pKa = 10.06RR21 pKa = 11.84MASKK25 pKa = 10.64NGRR28 pKa = 11.84KK29 pKa = 9.12VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6989

0

6989

2386252

29

6708

341.4

37.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.137 ± 0.035

0.687 ± 0.008

5.281 ± 0.023

6.551 ± 0.035

3.984 ± 0.023

7.977 ± 0.03

2.002 ± 0.013

5.905 ± 0.026

4.457 ± 0.028

9.97 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.747 ± 0.015

3.474 ± 0.023

4.239 ± 0.019

3.603 ± 0.017

5.79 ± 0.033

5.943 ± 0.023

5.43 ± 0.027

6.998 ± 0.023

1.38 ± 0.013

3.445 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski