Stenotrophomonas phage Pokken

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Pokkenvirus; Stenotrophomonas virus Pokken

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A5B9N9E9|A0A5B9N9E9_9CAUD Uncharacterized protein OS=Stenotrophomonas phage Pokken OX=2596674 GN=CPT_Pokken_013 PE=4 SV=1
MM1 pKa = 7.35SRR3 pKa = 11.84EE4 pKa = 3.87ADD6 pKa = 3.58CFEE9 pKa = 4.37PARR12 pKa = 11.84SYY14 pKa = 11.41DD15 pKa = 2.76IGTYY19 pKa = 9.34LWSIGSQVASPEE31 pKa = 3.53MDD33 pKa = 3.31QIKK36 pKa = 10.28EE37 pKa = 4.01IIQFPVVTEE46 pKa = 4.15TAQFGFNQLNSSYY59 pKa = 11.17EE60 pKa = 4.2DD61 pKa = 3.54YY62 pKa = 10.85CKK64 pKa = 10.4QSSFPAEE71 pKa = 4.93DD72 pKa = 3.12IDD74 pKa = 4.33VCVFNHH80 pKa = 6.66KK81 pKa = 10.29

Molecular weight:
9.25 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A5B9N599|A0A5B9N599_9CAUD Uncharacterized protein OS=Stenotrophomonas phage Pokken OX=2596674 GN=CPT_Pokken_050 PE=4 SV=1
MM1 pKa = 7.99LDD3 pKa = 3.35TMVSTRR9 pKa = 11.84TKK11 pKa = 10.29RR12 pKa = 11.84INPLQPEE19 pKa = 4.33KK20 pKa = 11.06PEE22 pKa = 3.84IDD24 pKa = 3.34YY25 pKa = 11.01SIYY28 pKa = 10.22PEE30 pKa = 4.09PPKK33 pKa = 10.01PRR35 pKa = 11.84KK36 pKa = 9.2RR37 pKa = 11.84KK38 pKa = 8.42HH39 pKa = 6.16PYY41 pKa = 9.82RR42 pKa = 11.84VMLDD46 pKa = 3.31HH47 pKa = 7.81PKK49 pKa = 10.03RR50 pKa = 11.84QHH52 pKa = 6.21LPEE55 pKa = 3.95PHH57 pKa = 6.38KK58 pKa = 11.1QFDD61 pKa = 3.51TGLTRR66 pKa = 11.84RR67 pKa = 11.84NRR69 pKa = 11.84ARR71 pKa = 3.36

Molecular weight:
8.63 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

23772

31

3522

255.6

28.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.389 ± 0.517

0.732 ± 0.128

6.133 ± 0.128

6.125 ± 0.22

3.559 ± 0.146

7.05 ± 0.245

1.994 ± 0.173

5.418 ± 0.215

5.763 ± 0.249

8.274 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.877 ± 0.106

4.943 ± 0.172

4.413 ± 0.207

4.711 ± 0.282

5.056 ± 0.21

5.927 ± 0.167

6.436 ± 0.282

6.588 ± 0.2

1.38 ± 0.123

3.231 ± 0.162

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski