Stenotrophomonas phage Pokken
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B9N9E9|A0A5B9N9E9_9CAUD Uncharacterized protein OS=Stenotrophomonas phage Pokken OX=2596674 GN=CPT_Pokken_013 PE=4 SV=1
MM1 pKa = 7.35 SRR3 pKa = 11.84 EE4 pKa = 3.87 ADD6 pKa = 3.58 CFEE9 pKa = 4.37 PARR12 pKa = 11.84 SYY14 pKa = 11.41 DD15 pKa = 2.76 IGTYY19 pKa = 9.34 LWSIGSQVASPEE31 pKa = 3.53 MDD33 pKa = 3.31 QIKK36 pKa = 10.28 EE37 pKa = 4.01 IIQFPVVTEE46 pKa = 4.15 TAQFGFNQLNSSYY59 pKa = 11.17 EE60 pKa = 4.2 DD61 pKa = 3.54 YY62 pKa = 10.85 CKK64 pKa = 10.4 QSSFPAEE71 pKa = 4.93 DD72 pKa = 3.12 IDD74 pKa = 4.33 VCVFNHH80 pKa = 6.66 KK81 pKa = 10.29
Molecular weight: 9.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.942
IPC2_protein 4.228
IPC_protein 4.101
Toseland 3.923
ProMoST 4.228
Dawson 4.062
Bjellqvist 4.215
Wikipedia 3.961
Rodwell 3.935
Grimsley 3.834
Solomon 4.05
Lehninger 3.999
Nozaki 4.19
DTASelect 4.342
Thurlkill 3.961
EMBOSS 3.973
Sillero 4.215
Patrickios 0.846
IPC_peptide 4.05
IPC2_peptide 4.19
IPC2.peptide.svr19 4.107
Protein with the highest isoelectric point:
>tr|A0A5B9N599|A0A5B9N599_9CAUD Uncharacterized protein OS=Stenotrophomonas phage Pokken OX=2596674 GN=CPT_Pokken_050 PE=4 SV=1
MM1 pKa = 7.99 LDD3 pKa = 3.35 TMVSTRR9 pKa = 11.84 TKK11 pKa = 10.29 RR12 pKa = 11.84 INPLQPEE19 pKa = 4.33 KK20 pKa = 11.06 PEE22 pKa = 3.84 IDD24 pKa = 3.34 YY25 pKa = 11.01 SIYY28 pKa = 10.22 PEE30 pKa = 4.09 PPKK33 pKa = 10.01 PRR35 pKa = 11.84 KK36 pKa = 9.2 RR37 pKa = 11.84 KK38 pKa = 8.42 HH39 pKa = 6.16 PYY41 pKa = 9.82 RR42 pKa = 11.84 VMLDD46 pKa = 3.31 HH47 pKa = 7.81 PKK49 pKa = 10.03 RR50 pKa = 11.84 QHH52 pKa = 6.21 LPEE55 pKa = 3.95 PHH57 pKa = 6.38 KK58 pKa = 11.1 QFDD61 pKa = 3.51 TGLTRR66 pKa = 11.84 RR67 pKa = 11.84 NRR69 pKa = 11.84 ARR71 pKa = 3.36
Molecular weight: 8.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 9.765
IPC_protein 10.467
Toseland 10.76
ProMoST 10.657
Dawson 10.847
Bjellqvist 10.526
Wikipedia 11.038
Rodwell 11.155
Grimsley 10.891
Solomon 10.95
Lehninger 10.921
Nozaki 10.716
DTASelect 10.526
Thurlkill 10.745
EMBOSS 11.14
Sillero 10.76
Patrickios 10.906
IPC_peptide 10.95
IPC2_peptide 9.209
IPC2.peptide.svr19 8.734
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
93
0
93
23772
31
3522
255.6
28.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.389 ± 0.517
0.732 ± 0.128
6.133 ± 0.128
6.125 ± 0.22
3.559 ± 0.146
7.05 ± 0.245
1.994 ± 0.173
5.418 ± 0.215
5.763 ± 0.249
8.274 ± 0.216
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.877 ± 0.106
4.943 ± 0.172
4.413 ± 0.207
4.711 ± 0.282
5.056 ± 0.21
5.927 ± 0.167
6.436 ± 0.282
6.588 ± 0.2
1.38 ± 0.123
3.231 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here